Abstract
Planting non-native tree species, like Douglas fir in temperate European forest systems, is encouraged to mitigate effects of climate change. However, Douglas fir monocultures often revealed negative effects on forest biota, while effects of mixtures with native tree species on forest ecosystems are less well understood. We investigated effects of three tree species (Douglas fir, Norway spruce, native European beech), on ground beetles in temperate forests of Germany. Beetles were sampled in monocultures of each tree species and broadleaf-conifer mixtures with pitfall traps, and environmental variables were assessed around each trap. We used linear mixed models in a two-step procedure to disentangle effects of environment and tree species identity on ground beetle abundance, species richness, functional diversity and species assemblage structure. Contradictory to our expectations, ground beetle abundance and functional diversity was highest in pure Douglas fir stands, while tree mixtures showed intermediate values between pure coniferous and pure beech stands. The main drivers of these patterns were only partially dependent on tree species identity, which highlights the importance of structural features in forest stands. However, our study revealed distinct shifts in assemblage structure between pure beech and pure Douglas fir stands, which were only partially eased through mixture planting. Our findings suggest that effects of planting non-native trees on associated biodiversity can be actively modified by promoting beneficial forest structures. Nevertheless, integrating non-native tree species, even in mixtures with native trees, will invariably alter assemblage structures of associated biota, which can compromise conservation efforts targeted at typical species composition.
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Introduction
Human impact is increasingly altering ecosystems worldwide, with wide-ranging consequences for biodiversity and ecosystem services (Cardinale et al. 2012; Dirzo et al. 2014). Studies have reported noticeable declines in the abundance and local species richness of both vertebrates and invertebrates (Hallmann et al. 2017; Rosenberg et al. 2019; Klink et al. 2020). Although such declines are often particularly pronounced in agricultural systems with high land use intensity, recent research has shown that biodiversity loss is also happening in forests (Seibold et al. 2019). Reasons for declining forest biodiversity are less well understood for European forests, where forest management in many cases has shifted towards more nature-oriented practices over the last decades (Borrass et al. 2017; Gustafsson et al. 2020). Nevertheless, a scarcity of key resources, such as deadwood, and extensive monoculture planting of tree species outside of their natural range might have contributed to the observed patterns (Hilmers et al. 2018; Ampoorter et al. 2020).
Despite positive trends towards more nature-friendly management, threats to biodiversity may persist in the future. Reasons for this include the promotion of potentially climatically better adapted, non-native tree species due to climate change (Bindewald et al. 2019). Such non-native tree species are often characterized by an impoverished associated biodiversity (Kennedy and Southwood 1984) and their increasing use in many regions has spurred a wealth of studies investigating their impact on native biodiversity (e.g. Finch and Szumelda 2007; Irwin et al. 2014; Schuldt and Scherer-Lorenzen 2014; Oxbrough et al. 2016; Matevski and Schuldt 2020). These studies often revealed locally negative effects of non-native tree species on the abundance, composition and species richness of forest biota (e.g. Finch and Szumelda 2007; Schuldt and Scherer-Lorenzen 2014). In Central Europe, particular attention has been paid to North American Douglas fir (Pseudotsuga menziesii), which is considered one of the most important tree species to address the economic challenges imposed by climate change in this region (Schmid et al. 2014). Previous studies have shown that Douglas fir monocultures can have lower arthropod biomass (Finch and Szumelda 2007; Schuldt and Scherer-Lorenzen 2014), bird activity (Gossner and Utschick 2004), and microbial biomass (Lu and Scheu 2020), compared to stands of native tree species. However, other taxa were less affected (e.g. crown-dwelling beetles; see Gossner and Simon (2002)) or even benefitted from Douglas fir stands (e.g. vascular plants, which may benefit from light availability and favourable soil conditions), especially when compared to the influence of Norway spruce (Picea abies) (Schmid et al. 2014), another coniferous species planted outside of its native range throughout larger parts of Europe. Moreover, potential negative effects of non-native tree species on biodiversity might be attenuated when planted in lower mixture proportions together with native tree species (Oxbrough et al. 2016). Tree species mixtures are considered an important option to adapting forests to changing climate (Ammer 2019), and mixtures of native and non-native tree species might therefore be a way to combine economic demands and biodiversity conservation in an integrated fashion. However, our current knowledge base for a clear understanding of the ecological consequences of mixing non-native and native tree species is still limited for many of the most relevant species, such as Douglas fir (Schmid et al. 2014). Moreover, few studies to date have looked at the functional diversity of associated biota in this context (e.g. Gallé et al. 2018; Martello et al. 2018), although this component of biodiversity is key to understanding how the ecological roles of assemblages are altered by human impact on ecosystems (Cardinale et al. 2012).
Here, we test how pure stands and two-species mixtures of three tree species varying in their degree of naturalness in Central European forests (non-native Douglas fir, Norway spruce planted outside its natural range, European beech, Fagus sylvatica, as the native tree species dominating under natural conditions) affect the abundance, species richness, functional diversity and species assemblage structure of ground beetles (Coleoptera: Carabidae). Our study was conducted on 20 study plots at four study sites in different regions of northern Germany. Ground beetles are a species-rich and well-studied group of arthropods sensitive to small-scale environmental conditions such as microclimate and habitat structure (Thiele 1977; Butterfield et al. 1995; Cameron and Leather 2012), which are altered by tree species composition. They support distinct communities in different habitats (Thiele 1977), thus making them well-suited indicators of habitat quality and targets for biodiversity conservation. We hypothesized that i) ground beetle abundance, species richness and functional diversity is lower in coniferous stands than in beech stands, as we expect greater microhabitat variability in beech stands, due to more spacious litter layer and higher availability of deadwood (e.g. Koivula et al. 1999). Negative effects of conifers compared to deciduous trees on ground beetle species richness and abundance were previously shown for other forest types (Elek et al. 2001; Barsoum et al. 2014; Oxbrough et al. 2016). Furthermore, we expect particularly strong negative effects of Douglas fir, as it is least associated with the native fauna. Negative effects of non-native Douglas fir have been shown for other epigeic arthropods (Finch and Szumelda 2007; Schuldt and Scherer-Lorenzen 2014), which might reduce prey availability for predatory arthropods such as ground beetles. However, we hypothesized that ii) mixtures with native European beech attenuate negative effects of admixing either of the two conifer species and might even lead to highest diversity due to the mixing of functionally very dissimilar tree species.
Material and methods
Study area and sampling design
Our study was conducted at four temperate forest sites in Lower Saxony, Germany (‘Harz’ (10.39707,51.77015), ‘Dassel’ (9.704126,51.72592), ‘Winnefeld’ (9.572035,51.66449), and ‘Nienover’ (9.529744,51.69858) (Fig. 1). All study sites are in the transition from Maritime to Continental climate (mean annual temperature 6.8 °C in Harz and 7.4 °C on the other sites and mean annual precipitation 1397 mm in Harz and 1119 mm on the other sites (DWD 2018). Occurring bedrock on sites varies, with greywacke and argillaceous schist formations in Harz and lime- and sandstone formations on all other sites (NIBIS 2018). At each site, five study plots of 50 m × 50 m representing five different stand types were established: (1) pure Douglas-fir (Pseudotsuga menziesii), (2) pure European beech (Fagus sylvatica), (3) pure Norway spruce (Picea abies), (4) mixture of European beech and Douglas-fir, and (5) mixture of European beech and Norway spruce. Distances between plots within sites ranged from minimal 88 m to max 3042 m with a mean distance of 1021 m. Detailed information about stand characteristics of all study plots are displayed in Table S1 in the supplementary material. All studied stands were managed uniformly by the Lower Saxony state forest enterprise.
Ground beetle sampling took place between 23rd of March and 1st of September 2019. At each study plot 12 pitfall traps were placed in a grid of 3 × 4 with 10 m distance among traps. Traps consisted of transparent plastic cups with an upper diameter of 9.4 cm and a depth of 10 cm. Cups were buried flush to soil level and covered with metal mesh (mesh width 1.5 cm) to minimize captures of non-target vertebrates. Utilized trapping solution was 150 mL of a 50% propylene glycol solution with an added odourless detergent to reduce surface tension. Traps were emptied in three-week intervals, resulting in 1920 total samples. Ground beetles were identified according to Müller-Motzfeld (2006), following the nomenclature used therein.
Functional traits
We assembled functional traits of ground beetles from published literature and data bases (Table 1) to quantify functional diversity of the ground beetle assemblages. Ten traits were chosen with the aim of characterising differences in habitat use and ecological strategies. When contradictory information was found, information related to populations close to the study area was preferably used, otherwise the most recent source was selected. For species where no data on specific traits were available, data from the next closest relative were used if it seemed plausible, otherwise this trait was left empty (for a complete list of species with their affiliated traits see Table S2 Supplementary Information).
Habitat characteristics
To identify influences of environmental factors that might drive differences in ground beetle assemblages among forest stand types, we selected nine habitat characteristics that have previously been shown to potentially affect ground beetles. Habitat characteristics were: (1) canopy openness, which alters the light regime and therefore also ground temperature and humidity in forests, strongly affecting ground beetle community assemblages (Thiele 1977; Molnár et al. 2001), (2) litter cover, indicating the presence and amount of litter around traps, which alters habitat structure and may provide higher abundance of prey, e.g. for ground beetles preying on collembola (Koivula et al. 1999), (3) litter depth, if increasing, depth provides a more three-dimensional habitat structure that can provide shelter for ground beetles and more niches for potential prey (Pearce et al. 2003), (4) herb cover, impacting microclimatic conditions such as soil moisture and temperature, but compared to canopy cover creating a more small-scale mosaic structure of varying conditions, (5) herb layer structure, which has been shown to impact ground beetles more than species identity, mostly due to altering environmental resistance, affecting mainly forest species which may not be adapted to such conditions (Elek et al. 2001), (6) deadwood cover, indicating the presence of smaller deadwood objects in our study, which, if present in high abundance, can restrict movement of ground beetles due to higher environmental resistance, but can also promote presence of alternative prey, (7) deadwood volume, which provides suitable niches for overwintering and reproduction sites for many ground beetles (Pearce et al. 2003), (8) deadwood decay class, due to varying possibilities of potential prey to colonize deadwood in different decay stages (Andringa et al. 2019) and differences in suitability as habitat for overwintering and (9) soil pH, because many ground beetles show preferences between acidity of the soil (Thiele 1977; Butterfield et al. 1995), and a majority of prey from ground beetles are also sensitive to soil pH, which can further affect abundance and species richness (Elek et al. 2001). Stand age was not included as a variable, as it was dependent on stand type in our study. Measurements of all habitat characteristics besides soil pH took place in the summer of 2019 across a 10 m × 10 m grid with traps in the centre of each square.
Deadwood was categorized in fine (branches) and coarse lying deadwood, using 7 cm diameter at thickest point as a dividing factor. Branch deadwood was quantified using visual cover estimation. Coarse lying deadwood (including tree stumps) were assessed by measuring diameter and length of each piece of dead wood and estimating stage of decay into four classes as suggested by Müller-Using (2005).
Cover estimations (as percent of covered area) were conducted visually for leaf litter and herb layer. To increase precision of estimations the area of each 10 × 10 m grid cell was divided into 4 quartiles.
Litter depth was measured along transects at 1, 2 and 4 m distance from each pitfall trap towards each corner of the measurement grid.
Herb layer structure was measured using a vegetation hurdle as described by Mühlenberg (1993). Vertical wire for measurement was set at 3 cm to include low growing forest vegetation important for ground-active arthropods. Measurements took place in the largest patch of herbaceous vegetation in each quartile of the sampling grid cells.
Canopy openness was recorded at the centre of each trap using a Solariscope (SOL 300, Ing.-Büro Behling, Hermannsburg).
Soil pH was measured between November 2017 and January 2018, using three soil cores (5 cm diameter) from 0 to 5 cm organic soil (see Lu and Scheu 2020 for details). Seven of the 20 study plots suffered from storm damage and were replaced with close-by alternatives in 2018 (before sampling of ground beetles). Therefore, not all soil pH samples are directly from the studied plots but from surrounding areas of the same stand type.
Data analysis
All analyses were carried out in R 3.6.2 (R Core Team 2018). Data was pooled per trap over the complete sampling period and all analyses were conducted on trap level. To ensure we are comparing samples of equal quality and equal completeness we used coverage-based rarefaction curves from incidence frequency data as proposed by Chao and Jost (2012). For this purpose, we used data generated by the iNEXT command from the iNEXT package (Chao et al. 2014). Results showed high percentage of sample coverage for all stand types (Fig. S1 Supplementary Information).
To test for significant differences of environmental variables between stand types we used mean values per trap when multiple measurements were taken around each trap. We used linear mixed-effects models for data on plot level (soil pH) and for variables on trap level (canopy openness, litter cover, litter depth, herb cover, herb structure, tree distance, deadwood cover, deadwood volume). Study site (plot-level data) or study plot nested in study site (trap-level data) was added as a random effect to linear mixed-effects models and each environmental variable was tested in a separate model against stand type. We applied multiple comparisons with adjustment of p-values (Bonferroni correction) to the linear model results by a simultaneous inference procedure provided by the glht function in the multcomp package (Hothorn et al. 2008).
For the analyses of ground beetles, we focused on rarefied species richness, abundance and, as a commonly used measure of functional diversity, Rao´s quadratic entropy (Rao´s Q; Rao, 1982). Rarefied richness was calculated for a specified sample coverage of 0.8 using the estimateD function of the iNEXT package (Chao et al. 2014). Rao´s Q, which quantifies the mean dissimilarity in trait values among individuals of an assemblage, was calculated using the dbFD function of the FD package (Laliberte and Legendre 2010). We analysed these response variables in two steps using mixed-effects models with study plot nested in study site as a random effect. In the first step, we fit the mixed models with stand type, north and east coordinates of the study plots and elevation as the fixed effects, to assess the extent to which ground beetle diversity and abundance differed among stand types and geographical regions. Significant differences of response variables between stand types were tested with the glht function in the multcomp package, with adjustment of p-values (Bonferroni correction). In the second step, we replaced stand type by the environmental variables listed above. We chose this approach because not all environmental variables were independent of stand type and therefore collinear. To test for possible collinearity of environmental variables we used vifstep from the usdm package with a threshold of 4 (Naimi et al. 2014). In both steps, the Akaike information criterion (AICc) with small sample correction (Burnham and Anderson 2010) was then used, in a stepwise backward selection of explanatory variables, to select the candidate model with lowest global AICc and thus the largest Akaike weight (wi) as the most supported model. Mixed-effects models were fitted with Gaussian error distribution. Model residual plots were checked for homogeneity of variances and normality (Zuur et al. 2009). For improved model fit, rarefied richness and abundance were log-transformed. 38 pitfall traps were destroyed by wild boars during the course of the study (2% of total samples), with the highest destruction rate taking place in a pure beech plot where 9 traps were destroyed (9% of samples from this plot). We replaced missing abundance data for individual traps during specific sampling intervals by the mean abundance per trap of the respective plot and sampling period.
To identify indicator species for different stand types we used a multi-level pattern analysis from the indicspecies package (De Cáceres and Legendre 2009) with number of permutations set to 999. Moreover, we applied non-metric multidimensional scaling (NMDS) using the function metaMDS in the package vegan (Oksanen et al. 2012) to visualize the differences in assemblage composition of ground beetles between different stand types. Significant environmental variables were fitted to the ordination using the function envfit with 1000 permutations. Furthermore, we used permutational multivariate analysis of variance, provided by the adonis2 function of the vegan package (Oksanen et al. 2012) for all pairwise combinations of different stand types, and adjusted p-values with Bonferroni correction. We tested for multivariate homogeneity of group dispersions using the betadisper command from the vegan package (Oksanen et al. 2012), with a Bray–Curtis dissimilarity index. Tukey post-hoc test was used to test for significant differences of multiple comparisons of stand types.
To test whether the results are affected by non-forest or vagrant species, all analyses were also carried out with a dataset only containing forest-associated ground beetles (following the definition and list in Müller-Motzfeld 2001). However, the smaller sample set of forest ground beetles did not qualitatively change the results. Therefore, we focus the presentation of the results on the complete data set and present results for forest-associated beetles in the supplementary material (Tables S5– S6 & Figs S2 – S3 Supplementary Information).
Results
Environmental conditions
Litter depth differed significantly among stand types (Table 2). Litter depth was significantly higher in pure beech and mixed stands than in pure coniferous stands, with the only exception of pure spruce compared to beech / Douglas fir mixture where no significant difference could be observed. Herb cover was significantly lower in pure spruce than pure Douglas fir. Pure beech and mixed stands did not show significant variation in comparison to other stand types. The remaining environmental variables showed high variability also within stand types, which made differences among stand types less obvious (Table 2).
Ground beetles
Over the complete sampling period (162 days) a total of 13,164 individuals were collected, belonging to 49 different species from 24 genera (for a complete list of species see Table 4 in Appendix 1). The three most abundant species comprised 62% of the total catch (Abax parallelepipedus 24.7%, Abax ovalis 20.2%, Pterostichus burmeisteri 17.4%). The highest total number of species was found in pure Douglas fir stands (37 species), the lowest number in pure beech and spruce stands (both 29 species). Mixed stands showed intermediate values with 32 (beech / Douglas fir) and 31 (beech / spruce) species. The most abundant species showed higher dominance in pure beech and pure spruce than in other stand types (Fig. 2). The abundance distribution in pure beech also showed noticeably lower evenness among species than other stand types (Fig. 2).
Rarefied richness (6.22 ± 0.3 SE) and abundance (96.78 ± 7.9) of ground beetles were highest per plot in pure Douglas fir stands, with significant differences in rarefied richness to pure beech stands (3.46 ± 0.1) and in abundance to pure spruce (35.25 ± 2.6) and beech / spruce mixtures (41.21 ± 2.9) (Fig. 3a,b; Table S4 Supplementary Information). Differences in rarefied richness and abundance of other stand types in pairwise comparisons were non-significant. Functional diversity measured as Rao´s Q showed decreasing values from pure Douglas fir (0.085 ± 0.003) over pure spruce (0.074 ± 0.004), beech / spruce mixtures (0.063 ± 0.003), beech / Douglas fir mixtures (0.060 ± 0.002), to pure beech (0.045 ± 0.002) (Fig. 3c; Table S4 Supplementary Information).
When replacing stand type by environmental variables in the linear mixed-effects models, mainly litter layer and herb layer characteristics showed significant relationships with ground beetle species richness, abundance and functional diversity (Table 3). While effects of litter cover and litter depth were always negative (Fig. 4b,d,e,f), herb cover showed positive effects (Fig. 4a,c). Increasing herb cover was related to higher rarefied richness and higher abundance of ground beetles. On the contrary an increase in litter cover was correlated with a decline in abundance and functional diversity, while higher litter depth led to lower rarefied richness and lower functional diversity of ground beetles. Furthermore, deadwood volume showed significant positive effects on rarefied richness. Other environmental variables, e.g. canopy openness, herb structure and soil-pH showed marginally significant relationships with ground beetles (p ≤ 0.06, see Table 3).
The composition of the ground beetle species assemblages differed between stand types, with a clear separation of pure stands and intermediate compositions for mixtures in the NMDS ordination (Fig. 5). Coniferous stands (pure Douglas fir and pure spruce) showed a small overlap, but both were clearly separated from pure beech along the first axis of the NMDS. Pairwise permutational multivariate analysis of variance revealed significant differences in species composition between all stand types (p ≤ 0.03), except between mixtures (p = 0.44) and between pure beech and beech / Douglas fir mixture (p = 0.06) (Table S3 Supplementary Information). Species assemblage composition was significantly more homogenous in pure beech stands compared to all other stand types, likely due to the absence of open habitat generalists, which can appear in other stand types with less canopy closure (Figure S5, Supplementary Information). The overall species composition was related to differences in litter layer, herb layer, deadwood, canopy openness and soil-pH. Pure beech stands showed strong positive correlations with litter depth and litter cover, while they negatively correlated with canopy openness and herb cover. Pure Douglas fir stands were positively correlated with canopy openness. Indicator species analysis confirmed differences between stand types, with two characteristic indicator species for pure beech, one for beech / Douglas fir mixtures and 12 for pure Douglas fir stands (Fig. 5). Pure spruce and beech / spruce mixtures showed no characteristic indicator species.
Discussion
Our study shows that the effects of non-native tree species on forest biota are not necessarily negative per se, but may rather depend on the group of organism studied. Moreover and, as we discuss below, such effects are related to the developmental stage and the associated environmental conditions of individual forest stands. Main drivers of ground beetle diversity and community structure were environmental factors that are only partially dependent on the specific tree species composition (see also Felipe-Lucia et al. 2018). This indicates that better knowledge of key forest structures that promote biodiversity may also be used to improve the integration of non-native tree species into forest management strategies which are aiming to combine environmental suitability and economic stability under climate change.
Differences among forest types may be modified by forest management
Contradictory to our hypothesis, rarefied species richness and functional diversity of ground beetles were significantly higher in pure Douglas fir stands compared to pure beech, while differences to pure spruce were statistically insignificant. Furthermore, ground beetle abundance was significantly higher in pure Douglas fir than pure spruce. However, the observed species assemblage composition of ground beetles showed significant differences between all pure stands. Patterns for species richness, functional diversity and abundance can be explained by strong effects of environmental characteristics that varied across the forest stands. Herb cover was highest in pure Douglas fir stands and showed positive correlations with rarefied richness and abundance. While the general influence of herbaceous vegetation on ground beetles has been shown before (Spence and Niemelä 1994; Bortmann 1996; Pakeman and Stockan 2014), it can be argued that this relationship primarily reflects variation of local microsites, e.g. soil water retention and soil pH, as well as light conditions, which are important abiotic factors impacting ground beetle assemblages (Thiele 1977; Magura et al. 2003). In support of this assumption, effects of soil pH on abundance as well as of canopy openness on rarefied richness and functional diversity were marginally significant, but turned significant when analyses were repeated without herb cover as predictor (data not shown). Importance of soil characteristics, such as soil pH and soil water retention, on forest ground beetle assemblages has been shown before in studies by Irmler (2001) and Gruttke (2001). Coniferous stands of an intermediate age are often characterized by more open conditions than particularly beech stands of a similar age (Heinrichs et al. 2019). However, these environmental conditions and the consequences for biodiversity can vary across forest developmental stages and tree species. Schuldt and Scherer-Lorenzen (2014) found strong negative effects of young Douglas fir stands on spider communities compared to beech and oak, while Finch and Szumelda (2007) found that intermediate-aged Douglas fir plantations substantially altered the composition and diversity of several groups of arthropods, including ground beetles, when compared to oak. This means that an overall evaluation of the effects of non-native tree species, such as Douglas fir, needs to take the full spectrum of forest developmental stages into account. In addition, the size of forest stands may play a role by influencing ground beetle assemblages, as forest ground beetles with strong specialization and limited dispersal abilities may disappear with decreasing stand size and increasing isolation (Gruttke 2001; Irmler 2007). We were not able to test these effects in our study, and future studies should test for interactive effects of forest stand size and tree species composition.
A potential inference that can be drawn from our study is that forest management should try to promote conditions beneficial to associated biota. For ground beetles this could be tolerating or actively creating canopy gaps (see also Butterfield et al. 1995), if this goes along with increasing ground vegetation. Our study showed the significance of higher herb cover in increasing carabid species richness and functional diversity, which can be assumed to be influenced by canopy cover. Such forest structures, with a mosaic pattern of canopy gaps, are a general feature of many older forest stages that are commonly lacking under economically oriented forest management, where harvesting of trees takes place before more diverse forest structures can establish (Hilmers et al. 2018). This also applies to the availability of dead wood (Hunter 1999; Lassauce et al. 2011), which our study indicated to be an important factor for ground beetles as well. Previous studies have shown that dead wood can be an important habitat feature also for non-saproxylic organisms, as it modifies microsite conditions of climate and prey availability (including shelter for overwintering ground beetles; Seibold et al. 2016, Trautner 2017). Features such as light availability (through canopy gaps) and dead wood are not strictly dependent on individual tree species, meaning that forest management could actively utilize these features to improve habitat characteristics for forest biota (see also Felipe-Lucia et al. 2018). This can have implications for the planting of non-native trees in general, where consideration of modifications of the stand structure might help to better connect economic objectives and biodiversity conservation. For a more comprehensive picture, however, more knowledge across multiple taxa and multiple trophic levels is required to develop generalizable recommendations (see also Finch and Szumelda 2007), which should be an incentive for future studies.
Non-native tree species seem to benefit generalist species
Some of the environmental variables identified to explain differences in ground beetle communities among stand types were inherently associated with the dominant tree species of the forest stands. Therefore, tree species identity still plays an important role in structuring these communities. Notably, characteristics of the litter layer, which depend on the type of tree species-specific litter input (Dickinson and Pugh 1974), showed significant negative effects on all response variables. This finding is in contrast to Koivula et al. (1999) and Magura et al. (2005), who found significantly higher abundances and no significant differences in species richness of ground beetles with increased leaf litter amounts in boreal spruce stands in Finland and temperate spruce stands in Hungary. Our results for the effects of litter characteristics on ground beetles were consistent when analyses were repeated with only pure coniferous plots, confirming negative effects also for conifer monocultures (Fig. S4 Supplementary Information). Leaf litter can provide ground beetles with shelter protecting against evaporation and predators (Koivula et al. 1999). It also provides a variety of niches, which are important for numerous other arthropods, favouring a diverse community and therefore providing a higher abundance of prey for ground beetles (Koivula et al. 2002). However, similar to our study, Guillemain, et. al. (1997) found negative effects of litter depth on ground beetle abundance and species richness in a variety of different broadleaf forest stands in France. They attributed these effects to an increase in the proportion of forest specialists and the disappearance of ubiquitous species, which are poorly adapted to living in this environment with respect to locomotory abilities and foraging strategies (see also Finch 2005). In our study, the lower litter depth and litter cover observed for pure Douglas fir might similarly benefit a wider range of more ubiquitous ground beetle species and outweigh positive effects, like provision of shelter, on more specialized forest species. This is reflected in our data by differences in the total abundances of species described as ubiquitous by Müller-Motzfeld (2001), e.g. Pterostichus niger (pure Douglas fir 861; pure beech 22), Carabus nemoralis (pure Douglas fir 54; pure beech 1), when compared to, for example, the mountainous forest specialist Pterostichus burmeisteri (pure Douglas fir 138; pure beech 909). A loss of forest specialist and therefore a shift in species assemblages was also discussed before for several other arthropod taxa in Douglas fir stands (Kohlert and Roth 2000; Winter 2001; Goßner and Ammer 2006). Repeating our analyses with a reduced subset of only strict forest species of ground beetles showed a lower influence of litter layer, supporting the assumption that effects of litter layer are predominantly influencing non-forest ground beetles. In terms of biodiversity conservation, this means that Douglas fir plantations may not provide secondary habitat for more specialized species associated with native beech forests. This finding is further supported by the strongly deviating species composition of beech and Douglas fir stands revealed by NMDS analysis (and which also applies to stands of Norway spruce in our study; see also Finch 2005). On the other hand, high functional diversity of ground beetles in pure coniferous stands suggests that these stands may provide a suitable habitat for a wide range of functionally different ground-beetle species, which can contribute to biodiversity conservation in general (although not necessarily to conservation of the specific biodiversity of native forests).
Mixed-species stands with trade-offs for biodiversity
Mixing non-native with native tree species might be an option to improve both the conservation of typical biodiversity of native forests and economic yields under climate change. In our study, ground beetle species composition of the mixed stands was a mixture of the beetle composition of pure stands. This indicates that forest stands composed of non-native and native tree species can retain more of the typical associated forest species than pure stands of non-native trees. However, tree mixtures in our study also showed intermediate values for abundance and diversity, meaning that mixture effects were not additive and apparently did not lead to an increased availability of niches that would allow for the coexistence of the full set of species associated with coniferous and deciduous forest stands. These patterns can have implications for forest management at larger spatial scales. A previous study indicated that small-scale heterogeneity (such as created by mixtures of tree species in our study) of forest stands might lead to lower increases of regional diversity in several taxa than heterogeneity at larger scales (at the cost of more uniformity at smaller scales, such as monoculture stands in our study; Schall et al. 2018). Similarly, Heinrichs et al. (2019) found for plants and lichens, that stand-level mixtures do not necessarily promote landscape-level diversity. These findings suggest that the spatial scale at which mixing tree species and creating habitat heterogeneity takes place is a key research topic when it comes to developing sustainable management strategies. Future studies should take this into account also when evaluating the effects of non-native tree species on biodiversity, as such patterns might be tree species-specific (Heinrichs et al. 2019).
Conclusions
Overall, our study highlights that not all drivers of ground beetle abundance and diversity are dependent on tree species identity. Therefore, negative effects of integrating non-native tree species in forests could be mitigated by forest management strategies, e.g. by increasing deadwood amounts or promoting canopy gaps. However, our results indicate that generalist species might benefit more from such measures than more specialized species adapted to the specific environmental conditions of native forests. Thus, achieving high levels of species richness in forests containing non-native tree species does not necessarily mean that these forests can preserve native biodiversity and species composition to full extents. This problem might be alleviated to some extent by planting non-natives in mixtures with native tree species, but our study and previous research indicates that the spatial scale at which such mixing is implemented can strongly influence biodiversity patterns beyond the local scale of individual forest stands. The contrasting findings of our and previous studies with respect to the influence of Douglas fir on associated biota, which may in part be due to taxon-specific responses and shifts in environmental effects over the course of forest development, highlights that a clear understanding of the ecological consequences of planting non-native tree species will strongly benefit from more research aimed at integrating multiple taxa and multiple forest developmental stages.
Data availability
Data will be made available at https://www.bexis.uni-jena.de/Login/Account.aspx upon publication.
Code availability
Not applicable.
References
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Acknowledgements
We thank Christian Ammer, Serena Müller, Jonas Glatthorn, all members of the RTG 2300 consortium and the local foresters who coordinated and helped with the establishment and maintenance of the project. This work was supported by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—316045089/GRK2300.
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Open Access funding enabled and organized by Projekt DEAL. This work was funded by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 316045089/GRK2300.
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AS conceived the research, PK and DM collected the data, PK processed and analysed the data, PK drafted the initial version of the manuscript and all authors substantially contributed to later versions of the manuscript. All authors gave final approval for publication.
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Kriegel, P., Matevski, D. & Schuldt, A. Monoculture and mixture-planting of non-native Douglas fir alters species composition, but promotes the diversity of ground beetles in a temperate forest system. Biodivers Conserv 30, 1479–1499 (2021). https://doi.org/10.1007/s10531-021-02155-1
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DOI: https://doi.org/10.1007/s10531-021-02155-1