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Expression of the 5-enoylpyruvylshikimate-3-phosphate synthase domain from the Acremonium sp. aroM complex enhances resistance to glyphosate

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Abstract

Objective

To discover and isolate a glyphosate-resistant gene from a microorganism through gene mining.

Results

The full aroM gene from Acremonium sp. (named aroMA.sp.) was cloned using rapid amplification of cDNA ends. The transcriptional expression level of each domain increased significantly after glyphosate treatment in the aroMA.sp. complex and reached its maximum at 48 h. The aroA domain of the aroMA.sp. (named aroA A.sp.) was expressed in Escherichia coli BL21 (DE3) and the product was purified through Ni-NTA affinity chromatography. Furthermore, 45 KDa was indicated by SDS-PAGE and its enzyme activity was optimal at 30 °C and PH 7.0. The Ki/Km value of aroAA.sp. was 0.106, and the E. coli BL21 harboring aroAA.sp. could grow in the M9 minimal medium with 100 mM glyphosate.

Conclusion

The aroAA.sp. from the aroMA.sp. complex had high enzyme activity and glyphosate resistance. Therefore, this research offers a new strategy for improving glyphosate resistance using the aroA domain of the aroM complex in the fungi.

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Acknowledgements

This research is financially supported by the National Transgenic Major Program (2016ZX08004001-04).

Supporting information

on the primers used in the experiments is provided in Supplementary Table S1.

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Authors and Affiliations

  1. Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Feng Liu & Yueping Cao

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  1. Feng Liu
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  2. Yueping Cao
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Correspondence to Yueping Cao.

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The author declares no financial or commercial conflict of interest.

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Liu, F., Cao, Y. Expression of the 5-enoylpyruvylshikimate-3-phosphate synthase domain from the Acremonium sp. aroM complex enhances resistance to glyphosate. Biotechnol Lett 40, 855–864 (2018). https://doi.org/10.1007/s10529-018-2529-0

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