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Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b

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Abstract

The periplasmic proteome of recombinant E. coli cells expressing human interferon-α2b (INF-α2b) was analysed by 2D-gel electrophoresis to find the most altered proteins. Of some unique up- and down-regulated proteins in the proteome, ten were identified by MS. The majority of the proteins belonged to the ABC transporter protein family. Other affected proteins were ones involved in the regulation of transcription such as DNA-binding response regulator, stress-related proteins and ecotin. Thus, the production of INF-α2b acts as a stress on the cells and results in the induction of various transporters and stress related proteins.

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Acknowledgments

This work was supported by the Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia.

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Correspondence to Mohd Puad Abdullah.

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Heidary, S., Rahim, R.A., Eissazadeh, S. et al. Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b. Biotechnol Lett 36, 1479–1484 (2014). https://doi.org/10.1007/s10529-014-1504-7

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  • DOI: https://doi.org/10.1007/s10529-014-1504-7

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