Abstract
Histone deacetylases (HDACs) deacetylate lysine residues of histone and non-histone proteins and thereby regulate the cell-cycle, gene expression, and several other processes. We have analyzed the effects of HDAC1 on Runx2-mediated regulation of osteopontin (OPN) promoter activation and gene expression in mesenchymal progenitor C3h10t1/2 cells and show that co-expression of HDAC1 along with Runx2 results in down-regulation of Runx2-induced OPN mRNA expression during both the proliferation and differentiation stages of C3h10t1/2 cells. Luciferase assay results revealed that HDAC1 efficiently down-regulated Runx2-stimulated OPN promoter activity in a dose-dependent manner whereas TSA relieved the HDAC1-mediated repression and up-regulated the Runx2-induced OPN promoter activity and mRNA expression. In vivo HDAC1 co-localized and physically interacted with Runx2 and associated with the OPN promoter. Thus, HDAC1 not only plays a critical role in regulation of Runx2-stimulated expression of osteogenic genes, like OPN, but also regulate the proliferation and differentiation stages of mesenchymal progenitor cells, such as C3h10t1/2.
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Acknowledgments
W. Liu thanks Dr. T. Fujita for his encouragement. This study was supported by the Training Fund of NENU’S Scientific Innovation Project (NENU-STC08015) and partially supported by The Jilin Province Science and Technology Development Project (20040114).
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Zhongli Zhang and Vishwa Deepak are contributed equally to this work.
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Zhang, Z., Deepak, V., Meng, L. et al. Analysis of HDAC1-mediated regulation of Runx2-induced osteopontin gene expression in C3h10t1/2 cells. Biotechnol Lett 34, 197–203 (2012). https://doi.org/10.1007/s10529-011-0756-8
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DOI: https://doi.org/10.1007/s10529-011-0756-8