Abstract
P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.
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Arai F, Hirao A, Ohmura M, Sato H, Matsuoka S, Takubo K, Ito K, Koh GY, Suda T (2004) Tie2/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche. Cell 118:149–161
Bain G, Ray WJ, Yao M, Gottlieb DI (1994) From embryonal carcinoma cells to neurons: the P19 pathway. Bioessays 16:343–348
Edwards MK, Harris JF, McBurney MW (1983) Induced muscle differentiation in an embryonal carcinoma cell line. Mol Cell Biol 3:2280–2286
Korhonen J, Polvi A, Partanen J, Alitalo K (1994) The mouse tie receptor tyrosine kinase gene: expression during embryonic angiogenesis. Oncogene 9:395–403
Lugus JJ, Park C, Choi K (2005) Developmental relationship between hematopoietic and endothelial cells. Immunol Res 32:57–74
McBurney MW (1993) P19 embryonal carcinoma cells. Int J Dev Biol 37:135–140
McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ (1982) Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299:165–167
Newman PJ (1997) The biology of PECAM-1. J Clin Invest 100:S25–S29
Reyes M, Dudek A, Jahagirdar B, Koodie L, Marker PH, Verfaillie CM (2002) Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest 109:337–346
Sato TN, Tozawa Y, Deutsch U, Wolburg-Buchholz K, Fujiwara Y, Gendron-Maguire M, Gridley T, Wolburg H, Risau W, Qin Y (1995) Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature 376:70–74
van der Heyden MA, Defize LH (2003) Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation. Cardiovasc Res 58:292–302
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This work was supported by a grant (10574) of Seoul R&BD Program.
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Choi, SC., Choi, JH., Shim, WJ. et al. P19 Embryonal carcinoma cells: a new model for the study of endothelial cell differentiation. Biotechnol Lett 30, 1169–1175 (2008). https://doi.org/10.1007/s10529-008-9677-6
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DOI: https://doi.org/10.1007/s10529-008-9677-6