Abstract
We have hypothesized that human bone marrow-derived mesenchymal stem cells (BMMSCs), that are not osteogenically differentiated prior to implantation, would regenerate bone extensively in vivo once exogenous bone morphogenetic protein-2 (BMP-2) was delivered to the implantation site. BMP-2 released from heparin-conjugated poly(lactic-co-glycolic acid) (HCPLGA) scaffolds stimulates osteogenic differentiation of cultured BMMSCs. Upon implantation, undifferentiated BMMSCs on BMP-2-loaded HCPLGA scaffolds induce far more extensive bone formation than either undifferentiated BMMSCs or osteogenically differentiated BMMSCs on HCPLGA scaffolds. These BMP-2-loaded HCPLGA scaffolds could prove invaluable for in vivo regeneration of bone from undifferentiated human BMMSCs.
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This work was supported by a grant (SC 3220) from the Stem Cell Research Center of the 21st Century Frontier Program, which is funded by the Ministry of Science and Technology, Republic of Korea.
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Sun-Woong Kang and Wan-Geun La have contributed equally to this work.
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Kang, SW., La, WG., Kang, J.M. et al. Bone morphogenetic protein-2 enhances bone regeneration mediated by transplantation of osteogenically undifferentiated bone marrow-derived mesenchymal stem cells. Biotechnol Lett 30, 1163–1168 (2008). https://doi.org/10.1007/s10529-008-9675-8
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DOI: https://doi.org/10.1007/s10529-008-9675-8