Abstract
Various waste frying oils (WFOs) were evaluated as substrates for rhamnolipid production by Pseudomonas aeruginosa mutant EBN-8 in the presence or absence of rhamnolipid precursor, under single-/batch-fed conditions. Soybean WFO was the best substrate, producing 9.3 g rhamnolipid l−1 with the specific product yield of 2.7 g g−1 h, under batch-fed cultivation with the addition of rhamnolipid precursor. The surface tension of the cell-free culture broth (CFCB) was 29.1 mN m−1 and the interfacial tension against n-hexadecane was <1 mN m−1. The hydrocarbon/ CFCB systems showed the relative emulsion stability to be in the range of 89.7–92.3.
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Acknowledgements
The authors are thankful to the Higher Education Commission, Islamabad for funding for this research. Z.A. Raza is grateful to Mrs. G. Abbas for her encouragement, and help in collecting the WFOs samples.
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Raza, Z.A., Khan, M.S., Khalid, Z.M. et al. Production Kinetics and Tensioactive Characteristics of Biosurfactant from a Pseudomonas aeruginosa Mutant Grown on Waste Frying Oils. Biotechnol Lett 28, 1623–1631 (2006). https://doi.org/10.1007/s10529-006-9134-3
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DOI: https://doi.org/10.1007/s10529-006-9134-3