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Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis

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Abstract

Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+, CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture.

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Acknowledgements

This work was supported by the Inha Vision 2010 development program and also by ERC for Advanced Bioseparation Technology, KOSEF.

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Correspondence to Dong-Il Kim.

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Lim, S.M., Choi, Y.S., Shin, H.C. et al. Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis. Biotechnol Lett 27, 607–611 (2005). https://doi.org/10.1007/s10529-005-3625-5

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  • DOI: https://doi.org/10.1007/s10529-005-3625-5

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