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Biological control of tomato gray mold caused by Botrytis cinerea by using Streptomyces spp.

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Abstract

Streptomyces is a genus known for its ability to protect plants against many pathogens and various strains of this bacteria have been used as biological control agents. In this study, the efficacy of Streptomyces philanthi RM-1-138, S. philanthi RL-1-178, and Streptomyce mycarofaciens SS-2-243 to control various strains of Botrytis cinerea was evaluated both in vitro and in vivo. In vitro studies using confrontation tests on PDA plates indicated that the three strains of Streptomyces spp. inhibited the growth of 41 strains of B. cinerea. Volatile compounds produced by Streptomyces spp. had an influence on the growth of ten strains of B. cinerea while its culture filtrate at low concentration (diluted at 10−3) showed a complete inhibition (100%) of spore germination of B. cinerea strain BC1. A significant protection efficacy of tomato against B. cinerea was observed on both whole plant test (57.4%) and detached leaf test (60.1%) with S. philanti RM-1-138. Moreover, this antagonistic strain had a preventive and a curative effect. These results indicated that S. philanthi RM-1-138 may have the potential to control gray mold caused by B. cinerea on tomato but further work is required to enhance its efficacy and its survival in planta.

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Acknowledgements

The authors would like to thank the Office of the Higher Education Commission for a scholarship to Mr. Sawai Boukaew under the CHE-PhD Scholarship Program (23/2554) and Thailand Research Fund (RTA5780002).

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Authors and Affiliations

  1. College of Innovation and Management, Songkhla Rajabhat University, Songkhla, 90000, Thailand

    Sawai Boukaew

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 90112, Thailand

    Poonsuk Prasertsan

  3. Pathologie Végétale, INRA, 84140, Montfavet, France

    Sawai Boukaew, Claire Troulet & Marc Bardin

Authors
  1. Sawai Boukaew
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  2. Poonsuk Prasertsan
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  3. Claire Troulet
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  4. Marc Bardin
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Correspondence to Sawai Boukaew or Marc Bardin.

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Handling Editor: Jane Debode.

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Boukaew, S., Prasertsan, P., Troulet, C. et al. Biological control of tomato gray mold caused by Botrytis cinerea by using Streptomyces spp.. BioControl 62, 793–803 (2017). https://doi.org/10.1007/s10526-017-9825-9

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  • DOI: https://doi.org/10.1007/s10526-017-9825-9

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