Abstract
In this study, the concentration/activity of the immune components in the turbot (Scophthalmus maximus) seminal plasma at four time points with the intervals of 2 weeks has been summarized. The results showed that the concentrations of transferrin (TF), transforming growth factor-βs (TGF-βs), complement 3 (C3), IgM and interferon-1β (IL-1β) varied as experiment went on; it was the same with the activities of protease, lactate dehydrogenase and lysozyme. The correlation analysis showed that C3 and IgM were most positively related to motility and straight-line velocity (VSL)/curvilinear velocity (VCL), whereas protease was most negatively correlated with motility and VSL/VCL. This study highlighted the importance in the necessity and possibility in improving the sperm quality during the actual production.
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Abbreviations
- SPPs:
-
Seminal plasma proteins
- ROS:
-
Reactive oxygen species
- TF:
-
Transferrin
- SOD:
-
Super oxide dismutase
- GR:
-
Glutathione reductase
- LPO:
-
Lipid peroxidation
- TGF-β1:
-
Transforming growth factor-β1
- C3:
-
Complement 3
- VSL:
-
Straight-line velocity
- VCL:
-
Curvilinear velocity
- LA:
-
Lactic acid
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Acknowledgements
We are grateful to Qingdao General Aquaculture Co. Ltd. for providing us the male bloodstock of turbots used in this experiment, and we thank the staff for their assistance in collecting semen.
Funding
The whole study was funded by the Modern Agriculture Industry System Construction of Special Funds (CARS-50), National Natural Science Fund of China (3110188) and Provincial Natural Science Foundation of Shandong.
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Han, M.M., Ding, F.H. & Li, J. Characterization of the immunological factors in turbot (Scophthalmus maximus) seminal plasma and their correlation with sperm quality. Aquacult Int 27, 1859–1868 (2019). https://doi.org/10.1007/s10499-019-00435-w
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DOI: https://doi.org/10.1007/s10499-019-00435-w