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Effect of microencapsulated thyme essential oil on white spot virus-infected Litopenaeus vannamei

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Abstract

The present work was performed to investigate if feeding Litopenaeus vannamei with microencapsulated thyme essential oil (TEM, 1.05 g thyme essential oil per 100-g powder) adsorbed on commercial pellet feed was able to protect shrimps against white spot syndrome virus (WSSV) disease. Five treatments were tested: uninfected shrimp fed with commercial pelleted feed (TC, negative control), WSSV-infected shrimp fed with commercial pelleted feed (T1, positive control), WSSV-infected shrimp fed with commercial pelleted feed with 0.1% TEM (T2), WSSV-infected shrimp fed with commercial pelleted feed with 0.5% TEM (T3), and WSSV-infected shrimp fed with commercial pelleted feed with 1% TEM (T4). At 72 h post infection, phenoloxidase activity of shrimps treated with 1% TEM did not show significant differences with TC values but it was significantly higher than that of the other treatments (T1, T2, and T3). Moreover, shrimps treated with T4 presented absence of clinical signs of WSSV infection and their survival rate was significantly higher than that of T1, T2, and T3 treatments. Therefore, 1% TEM seemed to protect shrimps against WSSV symptoms. Using microencapsulated thyme essential oil may help to fight against WSSV in shrimp farms.

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Acknowledgments

In memoriam of Margareth Linde Athayde.

Funding

The authors would like to acknowledge FINEP - Recarcina, MinisterioEconomía y Competitividad project CTQ2014-54520-P and Departament d’Enginyeria Química from Universitat Rovira i Virgili for their financial support.

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Authors and Affiliations

  1. Departament d’Enginyeria Química, Universitat Rovira i Virgili, URV, Av. Paisos Catalans, 26, 43007, Tarragona, Spain

    O. Tomazelli Júnior & S. De Lamo Castellví

  2. Programa de Pós Graduação em Ciências Ambientais, Universidade Comunitária da Região de Chapecó, UNOCHAPECÓ, Av. Atílio Fontana, 591, Chapecó, SC, 89809-000, Brazil

    F. Kuhn & J. Dal Magro

  3. Empresa de Pesquisa e Extensão Rural de Santa Catarina, EPAGRI, Centro de Desenvolvimento da Aquicutura e Pesca, CEDAP, Av. Admar Gonzaga, 1188, Florianópolis, SC, 88010-970, Brazil

    P. J. Mendonça Padilha

  4. Centro de Pesquisa para Agricultura Familiar – EPAGRI/Cepaf, Caixa Postal 791, Chapecó, SC, 89801-970, Brazil

    C. Nunes Nesi

  5. Grup de Química Analítica Enològica i dels Aliments (QAea), Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, 43007, Tarragona, Spain

    M. Mestres

Authors
  1. O. Tomazelli Júnior
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  2. F. Kuhn
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  3. P. J. Mendonça Padilha
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  4. C. Nunes Nesi
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  5. M. Mestres
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  6. J. Dal Magro
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  7. S. De Lamo Castellví
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Corresponding author

Correspondence to S. De Lamo Castellví.

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Tomazelli Júnior, O., Kuhn, F., Mendonça Padilha, P.J. et al. Effect of microencapsulated thyme essential oil on white spot virus-infected Litopenaeus vannamei. Aquacult Int 26, 1459–1468 (2018). https://doi.org/10.1007/s10499-018-0296-5

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  • DOI: https://doi.org/10.1007/s10499-018-0296-5

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