Abstract
The use of antibiotics to curtail vibriosis, which is a major infectious disease, plaguing shrimp and prawn is rather becoming less effective and the need for a better alternative is expedient. The outer membrane proteins (OMPs) of V. alginolyticus were extracted, mixed with powdered commercial feed and fed to the prawns to evaluate its effect on growth performance and protective potential. Sixty prawns were divided into groups A, B and C of 10 prawns each, with two replicates in six (150 L) glass aquaria. Groups A, B and C were fed with OMPs mixed diet, with OMPs-Freund’s incomplete adjuvant mixed diet and OMPs or adjuvant free diet (control diet) respectively. All the prawns were weighed weekly, and haemolymph was collected to determine the total haemocyte count (THC) and phenoloxidase (PO) activity. At the end of the feeding trial, prawns were intramuscularly challenged with 50 μL of 107 CFU V. alginolyticus. The treated groups were significantly higher in growth performance and THC than the control group, but no significant difference between the groups in terms of PO activity and mortality rate. The study, however, submitted that oral administration of OMPs with or without adjuvant is a good growth promoter and has the potential for protection against vibriosis in giant freshwater prawn (Macrobrachium rosenbergii).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Azad IS, Panigrahi A, Gopal C, Paulpandi S, Mahima C, Ravichandran P (2005) Routes of immunostimulation vis-à-vis survival and growth of Penaeus monodon postlarvae. Aquaculture 248(1–4):227–234. https://doi.org/10.1016/j.aquaculture.2005.04.011
Bai N, Gu M, Zhang W, Xu W, Mai K (2014) Effects of β-glucan derivatives on the immunity of white shrimp Litopenaeus vannamei and its resistance against white spot syndrome virus infection. Aquaculture 426-427:66–73. https://doi.org/10.1016/j.aquaculture.2014.01.019
Barman D, Nen P, Mandal SC, Kumar V (2013) Immunostimulants for aquaculture health management. J Marine Sci Res Dev 3(3)
Cheng W, Wang CH (2001) The susceptibility of the giant freshwater prawn Macrobrachium rosenbergii to Lactococcus garvieae and its resistance to copper sulfate stress. Dis Aquat Org 47(2):137–144. https://doi.org/10.3354/dao047137
Chiu ST, Hsieh SL, Yeh SP, Jian SJ, Cheng W, Liu CH (2010) The increase of immunity and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii by feeding with selenium enriched-diet. Fish and Shellfish Immunology 29(4):623–629. https://doi.org/10.1016/j.fsi.2010.06.012
FAO. (2011). World aquaculture 2010. FAO Fisheries and Aquaculture Department. Technical paper (Vol. No. 500/1.). Retrieved from http://www.fao.org/docrep/014/ba0132e/ba0132e.pdf
Itami T, Asano M, Tokushige K, Kubono K, Nakagawa A, Takeno N, Nishimura H, Maeda M, Kondo M, Takahashi Y (1998) Enhancement of disease resistance of kuruma shrimp, Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum. Aquaculture 164(1):277–288. https://doi.org/10.1016/S0044-8486(98)00193-8
Johansson MW, Keyser P, Sritunyalucksana K, Söderhäll K (2000) Crustacean haemocytes and haematopoiesis. Aquaculture 191(1–3):45–52. https://doi.org/10.1016/S0044-8486(00)00418-X
Keysami MA, Mohammadpour M (2013) Effect of Bacillus subtilis on Aeromonas hydrophila infection resistance in juvenile freshwater prawn, Macrobrachium rosenbergii (de Man). Aquac Int 21(3):553–562
Keysami MA, Saad CR, Sijam K, Daud HM, Alimon AR (2007) Effect of Bacillus subtilis on growth development and survival of larvae Macrobrachium rosenbergii (de Man). Aquac Nutr 13(2):131–136. https://doi.org/10.1111/j.1365-2095.2007.00463.x
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685. https://doi.org/10.1038/227680a0
Lavilla-Pitogo CR, Leaño EM, Paner MG (1998) Mortalities of pond-cultured juvenile shrimp, Penaeus monodon, associated with the dominance of luminescent vibrios in the rearing environment. Aquaculture 164(1–4):337–349. https://doi.org/10.1016/S0044-8486(98)00198-7
Li CC, Yeh ST, Chen JC (2010) Innate immunity of the white shrimp Litopenaeus vannamei weakened by the combination of a Vibrio alginolyticus injection and low-salinity stress. Fish Shellfish Immunol 28(1):121–127. https://doi.org/10.1016/j.fsi.2009.10.003
Lightner, D. V. (2005). Biosecurity in shrimp farming: pathogen exclusion through use of SPF stock and routine surveillance. Journal of the World Aquaculture Society, 36(3), 229–248
Liu CH, Chen JC (2004) Effect of ammonia on the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus. Fish Shellfish Immunol 16(3):321–334. https://doi.org/10.1016/S1050-4648(03)00113-X
Maftuch, Prasetio E, Sudianto A, Rozik M, Nurdiyani R, Sanusi E et al (2013) Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fabricius.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus. SpringerPlus 2(1):432
Pati US, Srivastava SK, Roy SC, More T (1996) Immunogenicity of outer membrane protein of Pasteurella multocida in buffalo calves. Vet Microbiol 52(3–4):301–311. https://doi.org/10.1016/S0378-1135(96)00066-1
Purivirojkul W, Areechon N, Srisapoome P (2006) The effect of peptidoglycan on immune response in black tiger shrimp (Penaeus monodon Fabricius). Kasetsart J (Nat Sci) 187:181–187
Qian RH, Xiao ZH, Zhang CW, Chu WY, Wang LS, Zhou HH, Wei Yw, Yu L (2008) A conserved outer membrane protein as an effective vaccine candidate from Vibrio alginolyticus. Aquaculture 278(1–4):5–9. https://doi.org/10.1016/j.aquaculture.2008.03.010
Sabri MY, Zamri-Saad M, Mutalib a R, Israf D a, Muniandy N (2000) Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep. Vet Microbiol 73(1):13–23. https://doi.org/10.1016/S0378-1135(99)00205-9
Sahoo PK, Pillai BR, Mohanty J, Kumari J, Mohanty S, Mishra BK (2007) In vivo humoral and cellular reactions, and the fate of injected bacteria Aeromonas hydrophila in freshwater prawn Macrobrachium rosenbergii. Fish Shellfish Immunol 23(2):327–340. https://doi.org/10.1016/j.fsi.2006.11.006
Scholz U, Garcia Diaz G, Ricque D, Cruz Suarez LE, Vargas Albores F, Latchford J (1999) Enhancement of vibriosis resistance in juvenile Penaeus vannamei by supplementation of diets with different yeast products. Aquaculture 176(3-4):271–283. https://doi.org/10.1016/S0044-8486(99)00030-7
Smith VJ, Brown JH, Hauton C (2003) Immunostimulation in crustaceans: does it really protect against infection? Fish Shellfish Immunol 15(1):71–90. https://doi.org/10.1016/S1050-4648(02)00140-7
Smith VJ, Söderhäll K, Hamilton M (1984) β 1, 3-Glucan induced cellular defence reactions in the shore crab, Carcinus maenas. Comp Biochem Physiol A Physiol 77(4):635–639. https://doi.org/10.1016/0300-9629(84)90176-2
Söderhäll K, Cerenius L (1998) Role of the prophenoloxidase-activating system in invertebrate immunity. Curr Opin Immunol 10(1):23–28. https://doi.org/10.1016/S0952-7915(98)80026-5
Sung HH (1990) Enhancement of growth in tiger shrimp (Penaeus monodon) by bacterin prepared from Vibrio vulnificus. Bull Eur Ass Fish Pathol 10(4):98–99
Sung HH, Yang YL, & Song YL (1996). Enhancement of microbicidal activity in the tiger shrimp Penaeus monodon via immunostimulation. J Crust Biol 16(2):278-284
Sung H-H, Kuo P-A, Kao W (2000) Effect of lipopolysaccharide on in vitro phagocytosis hemocytes from giant freshwater prawn (Macrobrachium rosenbergii). Jpn Soc Fish Pathol 3(35)
Thakur AB, Vaidya RB, Suryawanshi SA (2003) Pathogenicity and antibiotic susceptibility of Vibrio species isolated from moribund shrimps. Indian J Marine Sci 32(1):71–75
Thangaviji V, Michaelbabu M, Anand SB, Gunasekaran P (2012) Microbial & Biochemical Technology Immunization with the Aeromonas OMP provides protection against Aeromonas hydrophila in goldfish (Carassius auratus). Microbial Biochem Technol 4(2):45–49
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76(9):4350–4354. https://doi.org/10.1073/pnas.76.9.4350
Tseng IT, Chen JC (2004) The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under nitrite stress. Fish Shellfish Immunol 17(4):325–333. https://doi.org/10.1016/j.fsi.2004.04.010
Wowor D, Ng PKL (2007) The giant freshwater prawns of the Macrobrachium rosenbergii species group (Crustacea: Decapoda: Caridea: Palaemonidae). Raffles Bull Zool 55(2):321–336
Acknowledgements
We would like to thank Mr. Alli, a farmer, and members of staff of Aquaculture Research Station UPM, Puchong, Selangor, for their excellent technical assistance.
Funding
This project was funded by the Ministry of Science, Technology and Innovation (MOSTI), Malaysia, under the vote no. 6363400.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ajadi, A., Sabri, M.Y., Dauda, A.B. et al. Growth enhancement and protective potential of feed-based outer membrane proteins against vibriosis in Macrobrachium rosenbergii. Aquacult Int 26, 673–684 (2018). https://doi.org/10.1007/s10499-018-0244-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10499-018-0244-4