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Effects of dietary Biogen and sodium butyrate on hematological parameters, immune response, and histological characteristics of Nile tilapia (Oreochromis niloticus) fingerlings

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Abstract

The present study was carried out to evaluate the use of Biogen and sodium butyrate (SB) as feed additives in the diet of Nile tilapia (Oreochromis niloticus) fingerlings, in two parallel experiments. Biogen was incorporated in isonitrogenous (35% crude protein) and isocaloric (19 MJ kg−1) diets at four levels (0.0 (control), 0.5, 1.0, and 2.0%), while SB was included at five levels (0.0 (control), 0.5, 1.0, 2.0, or 3.0%). The diets were fed to fingerling Nile tilapia (10.5 ± 0.5 g) at a daily rate of 4% of their body weight, three times a day, for 60 days. Except the lymphocytes and monocyte numbers in fish fed SB, hematological parameters, including red blood cell (RBC) hematocrit (Ht), hemoglobin (Hb), and white blood cells (WBC) were not significantly (P > 0.05) affected by dietary Biogen and SB. The lymphocytes number in Nile tilapia fed on SB increased with increasing SB up to 2% level, and decreased afterwards. Monocyte numbers showed irregular patterns. The activities of serum enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of fish fed diets containing Biogen or SB were not significantly affected by dietary treatments (P > 0.05). No structural differences of tilapia liver were detected between all Biogen treatments and sodium butyrate concentrations up to 1% with control. At concentrations of 2 and 3% sodium butyrate, liver steatosis increased leading to shrinked acentric nuclei. At a concentration of 2% Biogen, some glomerulus cells had fading cytoplasm. Concerning fish fed SB diets, the structure of kidney was the same as in control except at concentration of 3% SB, where the septum between cells disappeared. No changes in gill structure were noticed at all concentrations of Biogen and SB.

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  • 27 December 2017

    The original version of this article unfortunately contained an error in the order of author names and affiliations.

References

  • Abdelhamid AM, Khalil FF, El-Barbary MI, Zaki VH, Hussein HS (2002) Feeding Nile tilapia on Biogen to detoxify aflatoxic diets. Proceedings 1st Conference of Animal and Fish Prod., Mansoura, pp 207–230

  • Abu Elala NM, Ragaa NM (2015) Eubiotic effect of a dietary acidifier (potassium diformate) on the health status of cultured Oreochromis niloticus. J Adv Res 6:621–629

    Article  CAS  PubMed  Google Scholar 

  • Agouz HM, Anwer W (2011) Effect of Biogenand Myco-Ad on the growth performance of common carp (Cyprinus carpio) fed a mycotoxin contaminated aquafeed. J Fish Aquat Sci 6:334–345

    Article  CAS  Google Scholar 

  • Ashraf MA, Goda S (2008) Effect of dietary ginseng herb (Ginsana G115) supplementation on growth, feed utilization, and hematological indices of Nile tilapia, Oreochromis niloticus (L.), fingerlings. J World Aquac Soc 39(2):205–214

    Article  Google Scholar 

  • Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dolz H, Millanao A et al (2013) Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol 15:1917–1942

    Article  PubMed  Google Scholar 

  • Castillo S, Rosales M, Pohlenz C, Gatlin GM (2014) Effects of organic acids on growth performance and digestive enzyme activities of juvenile red drum Sciaenops ocellatus. Aquaculture 433:6–12

    Article  CAS  Google Scholar 

  • Culling CF (1983) Handbook of histopathological and histochemical staining, 3rd edn. Buterworth, London

    Google Scholar 

  • Decie S, Lewis S (1991) Practical haematology, 7th edn. Churchill Livingstone, London

  • Defoirdt T, Boon N, Sorgeloos P, Verstraete W, Bossier P (2009) Short-chain fatty acids and poly-β-hydroxyalkanoates: (new) biocontrol agents for a sustainable animal production. Biotechnol Adv 27:680–685

    Article  CAS  PubMed  Google Scholar 

  • Dibner JJ, Winter B (2002) Use of organic acids as a model study the impact of gut microflora on nutrition and metabolism. J Appl Poult Res 10:453–463

    Article  Google Scholar 

  • Duncan DB (1955) Multiple ranges and multiple T-test. Biometrics 11:1–42

    Article  Google Scholar 

  • Elam TA (2004) Effect of Biogen and Bio-Mos on growth performance, production and some biochemical changes in Oreochromis niloticus and Mugil cephalus. The First Sci. Conf. Fac. Vet. Med., Moshtohor, Benha-RasSedr

  • EL-Gohary MS, Mohamed SG, Khalil RH, EL-Banna S, Soliman MK (2005) Immunosuppressive effects of metrifonate on Oreochromis niloticus. Egypt J Aquat Res 31:448–458

    CAS  Google Scholar 

  • El-Sayed Ali T, Abdel-Aziz SH, El-Sayed AM, Zeid S (2014) Structural and functional effects of early exposure to 4-nonylphenol on gonadal development of Nile tilapia (Oreochromis niloticus): a-histological alterations in testes. Fish Physiol Biochem 40:1495–1507

    Article  CAS  PubMed  Google Scholar 

  • El-Sayed Ali T, Martinez-Llorens S, Monino A, Jover Cerda M, Tomas-Vidal A (2016) Effects of weekly feeding frequency and previous ration restriction on the compensatory growth and body composition of Nile tilapia fingerlings. Egypt J Aquat Res 42:357–363

    Article  Google Scholar 

  • El-Sayed A-FM, ElGhobashy AE, El-Mezayen MM (2013) Effects of feed colour on growth and feed utilization efficiency of Nile tilapia (Oreochromis niloticus L.) fry and fingerlings. Aquac Nutr 19:870–876

    Article  CAS  Google Scholar 

  • FAO (Food and Agriculture Organization of the United Nations) (2017). Global aquaculture production 1950–2015. Rome. http://www.fao.org/fishery/statistics/global-aquaculture-production/query/en

  • Hai NV (2015) Research findings from the use of probiotics in tilapia aquaculture: a review. Fish Shellfish Immunol 45:592–597

    Article  CAS  PubMed  Google Scholar 

  • Hai NV (2016) The use of probiotics in aquaculture. J Appl Microbiol 119:917–935

  • Hoseinifar SH, Zoheiri F, Caipang CM (2016) Dietary sodium propionate improved performance, mucosal and humoral responses in Caspian white fish (Rutilus frisii kutum) fry. Fish Shellfish Immunol 55:523–528

    Article  CAS  PubMed  Google Scholar 

  • Irianto A, Austin B (2002) Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 25:333–342

    Article  CAS  Google Scholar 

  • Khalafalla MME (2010) Growth response of Oreochromisniloticus fingerlings to diets containing different levels of Biogen. Agric Res Kafer El-Shiekh Univ 36(2):97–110

    Google Scholar 

  • Khattab YAE, Shalaby AME, Sharaf SM, El-Marakby HI, Rizkalla EH (2004) The physiological changes and growth performance of the Nile tilapia Oreochromis niloticus after feeding with Biogen as growth promoter. Egypt J Aquat Bio Fish 8:145–158

    Google Scholar 

  • Koh CB, Romano N, Zahrah AS, Ng WK (2016) Effects of a dietary organic acids blend and oxytetracycline on the growth, nutrient utilization and total cultivable gut microbiota of the red hybrid tilapia, Oreochromis sp., and resistance to Streptococcus agalactiae. Aquac Res 47:357–369

    Article  CAS  Google Scholar 

  • Lara-Flores M, Olivera-Castillo L, OlveraNovoa MA (2010) Effect of the inclusion of a bacterial mix (Streptococcus faecium and Lactobacillus acidophilus), and the yeast (Saccharomyces cerevisiae) on growth, feed utilization and intestinal enzymatic activity of Nile tilapia (Oreochromis niloticus). J Fish Aquac 2:93–101

    CAS  Google Scholar 

  • Li X, Cui W, Leng X (2017) Citric acid substituted the inclusion of inorganic phosphorus in diet of rainbow trout (Oncorhynchus mykiss). Aquac Res 48:1089–1098

    Article  CAS  Google Scholar 

  • Liebert F, Mohamed K, Lückstädt C (2010) Effects of diformates on growth and feed utilization of all male Nile Tilapia fingerlings (Oreochromis niloticus) reared in tank culture. XIV International Symposium on Fish Nutrition and Feeding, Qingdao, China, Book of Abstracts. p.190

  • Lim C, Klesius PH, Luckstadt C 2010 Effects of dietary levels of potassium diformate on growth, feed utilization and resistance to Streptococcus iniae of Nile tilapia, Oreochromis niloticus. Proceedings of the fourteenth International Symposium on Fish Nutrition and Feeding; 31 May–4 Jun, Qingdao

  • Macey BM, Coyne VE (2005) Improved growth rate and disease resistance in farmed Haliotismidae through probiotic treatment. Aquaculture 245:249–261

    Article  Google Scholar 

  • Maron D, Smith T, Nachman K (2013) Restrictions on antimicrobial use in food animal production: an international regulatory and economic survey. Glob Health 9:48

    Article  Google Scholar 

  • Mehrim AI (2009) Effect of dietary supplementation of Biogen (commercial probiotic) on mono-sex Nile tilapia Oreochromis niloticus under different stocking densities. J Fish Aquat Sci 4(6):261–273

    Article  CAS  Google Scholar 

  • Mohammad AR, Mohammad Z, Vahid Y, Seyed Mohammad M (2012) Effect of different levels of supplementation of Saccharomyces cerevisiae on growth performance, feed utilization and body biochemical composition of Nile tilapia (Oreochromis niloticus) fingerlings. J Persian Gulf 3(9):15–24

    Google Scholar 

  • Ng WK, Koh CB (2016) The utilization and mode of action of organic acids in the feeds of cultured aquatic animals. Rev Aquac 1:1–27. https://doi.org/10.1111/raq.12141

    Google Scholar 

  • Ng WK, Koh CB, Sudesh K, Siti-Zahrah A (2009) Effects of dietary organic acids on growth, nutrient digestibility and gut microflora of red hybrid tilapia, Oreochromis sp., and subsequent survival during a challenge test with Streptococcus agalactiae. Aquac Res 40:1490–1500

    Article  CAS  Google Scholar 

  • Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic pyruvic transaminases. Am J Clin Path 28:56–63

    Article  CAS  PubMed  Google Scholar 

  • Sahu S, Das BK, Pradhan J, Mohapatra BC, Mishra BK, Sarangi N (2007) Effect of magnifera indica kernel as a feed additive on immunity and resistance to Aeromonas hydrophila in Labeorohita fingerlings. Fish Shellfish Immunol 23:109–118

    Article  CAS  PubMed  Google Scholar 

  • Sahu MK, Swarnakumar NS, Sivakumar K, TThangaradjou T, Kannan L (2008) Probiotics in aquaculture: importance and future perspectives. Indian J Microbiol 48:299–308

    Article  PubMed  PubMed Central  Google Scholar 

  • Sankar H, Philip B, Philip R, Singh ISB (2017) Effect of probiotics on digestive enzyme activities and growth of cichlids, Etroplus suratensis (pearl spot) and Oreochromis mossambicus (tilapia). Aquac Nutr 23:852–864

    Article  CAS  Google Scholar 

  • Sihag R, Sharma P (2012) Probiotics: the new ecofriendly alternative measures of disease control for sustainable aquaculture. J Fish Aquat Sci 7:72–103

    Article  Google Scholar 

  • Silva BC, Vieira FN, Mourino JP, Ferreira GS, Seiffert WQ (2012) Salts of organic acids selection by multiple characteristics for marine shrimp nutrition. Aquaculture 384-387:104–110

    Article  Google Scholar 

  • Stoskopf MK (1993) Clinical pathology. In: Stoskopf MK (ed) Fish medicine, WB Saunders, Philadelphia, p 113–131

  • Soltan MA, El-Laithy SM (2008) Effect of probiotics and some spices as feed additives on the performance and behaviour of the Nile tilapia, Oreochromis niloticus. Egypt J Aquat Biol Fish 12(2):63–80

    Google Scholar 

  • Wang YB (2007) Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture 269:259–264

    Article  CAS  Google Scholar 

  • Zhou Z, Liu Y, He S, Shi P, Gao X, Yao B, Gao X, Yao B, Ringǿ E (2009) Effects of dietary potassium diformate (KDF) on growth performance, feed conversion and intestinal bacterial community of hybrid tilapia (Oreochromis niloticus ♀ x O aureus ♂). Aquaculture 291:89–94

    Article  CAS  Google Scholar 

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Correspondence to Tamer El-Sayed Ali.

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A correction to this article is available online at https://doi.org/10.1007/s10499-017-0221-3.

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El-Sayed Ali, T., El-Sayed, A.M., Eissa, M.AR. et al. Effects of dietary Biogen and sodium butyrate on hematological parameters, immune response, and histological characteristics of Nile tilapia (Oreochromis niloticus) fingerlings. Aquacult Int 26, 139–150 (2018). https://doi.org/10.1007/s10499-017-0205-3

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