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Dietary supplementation of acidifier: effect on growth performance and haemato-biochemical parameters in the diet of Cirrhinus mrigala juvenile

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Abstract

The dietary supplementation of acidifier in Cirrhinus mrigala juvenile was evaluated in terms of change in growth, autochthonous intestinal microbiota and haemato-biochemical parameters for period of 60 days. Acidifier was added at a level of 0 (control diet), 0.5, 1 and 1.5% to the practical pellet diet. At the end of the experiment, growth parameters, survival, bacillus bacterial population, haematological and immunological parameters were analysed. Higher WG%, SGR and PER and lower FCR (P < 0.05) was found in group fed 1.5% OA. Lysozyme activity and respiratory burst activity (nitroblue tetrazolium) were significantly affected by dietary 1.5% acidifier (P < 0.05). In fish fed on the diet with 1.5% acidifier showed a significant increase of total heterotrophic autochthonous bacterial and Bacillus levels (P < 0.05) compared with those fed on the diets supplemented with organic acid. In addition to increase in WBC, RBC, haemoglobin and platelets count levels were observed in this group. Relative % survival rate was found to be higher in the fish fed on 1.5% acidifier after challenging with Aeromonas hydrophilla bacteria. These results indicated that dietary supplementation of acidifier at a dose of 1.5% improved growth performance and enhanced the beneficial intestinal microbiota and stimulate immune response of Cirrhinus mrigala juvenile.

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Acknowledgements

The authors are grateful to Dr. W.S. Lakra, Director, Central Institute of Fisheries Education, Mumbai, for providing facilities for carrying out the work. The first author is grateful to the Indian Council of Agricultural Research, New Delhi for financial support.

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  1. Rohtak Centre, ICAR-Central Institute of Fisheries Education, Lahli, Haryana, 124411, India

    Pankaj Kumar, K. K. Jain, P. Sardar, N. P. Sahu & S. Gupta

  2. Department of Fish Nutrition, Biochemistry and Physiology, Central Institute of Fisheries Education, Fisheries University Road, Versova, Mumbai, 400061, India

    Pankaj Kumar, K. K. Jain, P. Sardar, N. P. Sahu & S. Gupta

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Correspondence to Pankaj Kumar.

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Kumar, P., Jain, K.K., Sardar, P. et al. Dietary supplementation of acidifier: effect on growth performance and haemato-biochemical parameters in the diet of Cirrhinus mrigala juvenile. Aquacult Int 25, 2101–2116 (2017). https://doi.org/10.1007/s10499-017-0176-4

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