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Effect of thiamine injection on growth performance, hematology and germinal vesicle migration in sterlet sturgeon Acipenser ruthenus L.

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Abstract

The present study aimed to investigate the effect of thiamine on growth, hematological, egg thiamine content and oocyte nucleus migration indices in sterlet sturgeon (Acipenser ruthenus). A total of 45 female fish (698.6 ± 8.9 g) were distributed in nine fiberglass tanks with three treatments (each in three replicates). Experimental fish were fed once a day with practical diet supplemented with 1 g kg−1 amprolium hydrochloride (as the antithiamine) for 5 months before spawning. Thiamine hydrochloride was injected to fish with one of three doses at 0, 5 and 50 mg kg−1 body weight (BW) at three different stages. At the end of each month, fish were weighed and growth parameters such as weight gain, specific growth rate, feed efficiency and final weight were determined. At the end of the 5-month period, hematological parameters and egg thiamine content were measured. The results showed no significant differences in terms of growth performance. Hemoglobin, number of red blood cells and white blood cells were not significantly different among the treatments, but hematocrit was significantly higher in fish injected with 5 mg kg−1 BW thiamine. The mean corpuscular volume and mean corpuscular hemoglobin were not significantly different, but lower value of mean corpuscular hemoglobin concentration was observed in fish injected with 5 mg thiamine. Percentage of lymphocytes, neutrophils, eosinophils and monocytes were not significantly different among treatments. The results of the germinal vesicle migration index at different stages showed no significant differences in different groups. Results showed that free thiamine, thiamine pyrophosphate and total thiamine in eggs significantly increased at 50 mg kg−1 injection dose compared with the control, but no significant different was found in thiamine monophosphate. The results revealed that thiamine injection to sterlet broodstocks has positive accumulation in egg thiamine content and some hematological parameters but have no effect on growth and ovarian maturation. We conclude that thiamine can affect some physiological features of broodstock, which needs to be specified in further studies.

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References

  • Agrawal NK, Mahajan CL (1983) Haematological and haematopoietic studies in pyridoxine deficient fish, Channa punctatus Bloch. J Fish Biol 22:91–103. doi:10.1111/j.1095-8649.1983.tb04729.x

    Article  CAS  Google Scholar 

  • Amcoff P, Borjeson H, Lindeberg J, Norrgren L (1998) Thiamine (vitamin B1) concentrations in feral Baltic salmon exhibiting the M74 syndrome. In: McDonald G, Fitzsimons JD, Honeyfield DC (eds) Early life stage mortality syndrome in fishes of the Great Lakes and Baltic Sea. Proceedings of the 126th annual meeting of the Am Fish Soc, 25–29 Aug. Am Fish Soc Symp 21, Dearborn, Michigan pp 82–89

  • Amcoff P, Akerman G, Borjeson H, Tjarnlund U, Norrgren L, Balk L (2000) Hepatic activities of thiamine hydrochloride-dependent enzymes, glucose-6-phosphate dehydrogenase and cytochrome P4501A in Baltic salmon (Salmo salar) yolk-sac fry after thiamine treatment. Aquat Toxicol 48:391–402. doi:10.1016/S0166-445X(00)00089-8

    Article  CAS  PubMed  Google Scholar 

  • Anderson JW, Murai T (1978) Thiamin requirement of channel catfish fingerling. J Nutr 108:176–180. doi:10.1016/0044-8486(88)90292-X

    Google Scholar 

  • Andrade JI, Ono EA, Cruz G, Menezes GC, Brasil EM, Roubach R, Urbinati EC, Tavares-Dias M, Marcon JL, Affonso EG (2007) Influence of diets supplemented with vitamins C and E on pirarucu (Arapaima gigas) blood parameters. Comp Biochem Physiol 146A:576–580. doi:10.1016/j.cbpa.2006.03.017

    Article  Google Scholar 

  • Berglund I (1995) Effects of spring temperature and feeding regime on sexual maturation in Atlantic salmon Salmo salar L. male parr. In: Goetz FW, Thomas P (eds) Reproductive physiology of fish, general and comparative endocrinology. Fish Symposium 95, Austin, Texas, pp 170–172

  • Billard R, Lecointre G (2001) Biology and conservation of sturgeon and paddlefish. Rev Fish Biol Fish 10:355–392. doi:10.1023/A:1012231526151

    Article  Google Scholar 

  • Birstein VJ (1993) Sturgeons and paddlefishes: threatened fishes in need of conservation. Conserv Biol 7:773–787. doi:10.1046/j.1523-1739.1993.740773.x

    Article  Google Scholar 

  • Bittencourt F, Souza BE, Lui TA, Borella MI, Boscolo WR, Feiden A, Romagosa E (2012) Protein diets promote the maturation of oocytes and spawning of Piaractus mesopotamicus kept in cages. J Appl Ichthyol 28:886–893. doi:10.1111/jai.12060

    Article  CAS  Google Scholar 

  • Blaxhall PC, Daisley KW (1973) Routine haematological methods for use with fish blood. J Fish Biol 5:771–781. doi:10.1111/j.1095-8649.1973.tb04510.x

    Article  Google Scholar 

  • Borjeson H, Amcoff P, Ragnarsson B (1999) Reconditioning of sea-run Baltic salmon (Salmo salar) that have produced progeny with the M74 syndrome. Ambio 28:30–36

    Google Scholar 

  • Brown SB, Fitzsimons JD, Palace VP, Vandenbyllaardt L (1998) Thiamine and early mortality syndrome in lake trout. Am Fish Soc Symp 21, 28 Aug, Dearborn, Michigan, USA

  • Carvalho PSM, Tillitt DE, Zajicek JL, Dale RA, Honeyfield DC, Fitzsimons JD, Brown SB (2009) Thiamine deficiency effects on the vision and foraging ability of lake trout fry. J Aquat Anim Health 21:315–325. doi:10.1577/H08-025.1

    Article  PubMed  Google Scholar 

  • Chapman FA, Van Eenennaam JP (2007) Sturgeon aquaculture - specialized techniques: determining the stage of sexual maturity in female sturgeon for artificial spawning: the egg polarization index or PI. Fisheries and Aquatic Sciences Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida

  • Chen R, Lochmann R, Goodwin A, Praveen K, Dabrowski K, Lee K (2004) Effects of dietary vitamins C and E on alternative complement activity, hematology, tissue composition, vitamin concentrations and response to heat stress in juvenile golden shiner (Notemigonus crysoleucas). Aquaculture 242:553–569. doi:10.1016/j.aquaculture.2004.09.012

    Article  CAS  Google Scholar 

  • Coles EH (1986) Remove from marked records. In: Saunders WB (ed) Veterinary clinical pathology. Saunders Company, Philadelphia, pp 10–42

    Google Scholar 

  • Dakshinamurti K, Chauhan J (1994) Binding proteins for a-tocopherol, L-ascorbic acid, thiamine, and vitamin B6. In: Dakshinamurti K (ed) Vitamin receptors: vitamins as ligands in cell communication. Cambridge University Press, New York, pp 190–193

    Chapter  Google Scholar 

  • Dettlaff TA, Ginsburg AS, Schmalhausen OI (1993) Sturgeon fishes; Developmental biology and aquaculture. Springer, Berlin, 300 pp

  • Drabkin DR (1945) Crystallographic and optical properties of human hemoglobin: a proposal for the standardization of hemoglobin. Am J Med Sci 209:268–270. doi:10.1126/science.108.2792.15

    CAS  Google Scholar 

  • Falahatkar B, Efatpanah Komaee I (2011) Egg extraction of sterlet sturgeon, Acipenser ruthenus, through surgery. J Vet Res 4:349–353

    Google Scholar 

  • Falahatkar B, Akhavan SR, Efatpanah I, Meknatkhah B (2013) Effect of winter feeding and starvation on the growth performance of young-of-year (YOY) great sturgeon, Huso huso. J Appl Ichthyol 29:26–30. doi:10.1111/j.1439-0426.2012.02017.x

    Article  Google Scholar 

  • Feng L, Huang HH, Liu Y, Jiang WD, Hu K, Li SH (2011) Effect of dietary thiamin supplement on immune responses and intestinal microflora in juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Nutr 17:557–569. doi:10.1111/j.1365-2095.2011.00851.x

    Article  CAS  Google Scholar 

  • Fitzsimons JD, Williston B, Amcoff P, Balk L, Pecor C, Ketola HG, Hinterkopf JP, Honeyfield DC (2005) The effect of thiamine injection on upstream migration, survival, and thiamine status of putative thiamine hydrochloride-deficient coho salmon. J Aquat Anim Health 17:48–58. doi:10.1577/H04-003.1

    Article  Google Scholar 

  • Fitzsimons JD, Williston B, Williston G, Brown L, Shaarawi AE, Vandenbyllaardt L, Honeyfield D, Tillitt D, Wolgamood M, Brown SB (2007) Egg thiamine status of Lake Ontario salmonines, 1995–2004, with emphasis on lake trout. J Gt Lakes Res 33:93–103. doi:10.3394/0380-1330(2007)33[93:ETSOLO]2.0.CO;2

    Google Scholar 

  • Fitzsimons JD, Brown SB, Williston B, Williston G, Brown LR, Moore K, Honeyfield DC, Tillitt DE (2009) Influence of thiamine deficiency on lake trout larval growth, foraging, and predator avoidance. J Aquat Anim Health 21:302–314. doi:10.1577/H08-019.1

    Article  PubMed  Google Scholar 

  • Furuita H, Ishida T, Suzuki T, Unuma T, Kurokawa T, Sugita T, Yamamoto T (2009) Vitamin content and quality of eggs produced by broodstock injected with vitamins C and E during artificial maturation in Japanese eel Anguilla japonica. Aquaculture 289:334–339. doi:10.1016/j.aquaculture.2009.01.032

    Article  CAS  Google Scholar 

  • Fynn-Aikins K, Bowser P, Honeyfield DC, Fitzsimons J, Ketola HG (1998) Effect of dietary amprolium hydrochloride on tissue thiamin and Cayuga syndrome in Atlantic salmon. Trans Am Fish Soc 127:747–757. doi:10.1577/1548-8659(1998)127<0747:EODAOT>2.0.CO;2

    Article  CAS  Google Scholar 

  • Garcia F, Pilarski F, Onaka EM, de Moraes FR, Martins ML (2007) Hematology of Piaractus mesopotamicus fed diets supplemented with vitamins C and E, challenged by Aeromonas hydrophila. Aquaculture 271:39–46. doi:10.1016/j.aquaculture.2007.06.021

    Article  CAS  Google Scholar 

  • Ghazaly KS (1991) Influences of thiamin on lead intoxication, lead deposition in tissues and lead hematological responses of Tilapia zillii. Comp Biochem Physiol C 3:417–421. doi:10.1016/0742-8413(91)90018-O

    Article  Google Scholar 

  • Graff E, Waagbo R, Fivelstad S, Vermeer C, Lie O, Klundeby A (2002) A multivariate study on the effects of dietary vitamin K, vitamin D3 and calcium, and dissolved carbon dioxide on growth, bone minerals, vitamin status and health performance in smolting Atlantic salmon Salmo salar L. J Fish Dis 25:599–614. doi:10.1046/j.1365-2761.2002.00403.x

    Article  CAS  Google Scholar 

  • Granstrom DE (2003) Agricultural (non biomedical) animal research outside the laboratory: a review of guidelines for institutional animal care and use committees. ILAR 44:206–210. doi:10.1093/ilar.44.3.206

    Article  CAS  Google Scholar 

  • Halver JE (1972) The vitamins. In: Halver JE (ed) Fish nutrition. Academic Press, New York, pp 29–103

    Chapter  Google Scholar 

  • Hashimoto Y, Arai S, Nose T (1970) Thiamine deficiency symptoms experimentally induced in the eel. Bull Jpn Soc Sci Fish 36:791–797

    Article  CAS  Google Scholar 

  • Honeyfield DC, Hinterkopf JP, Fitzsimons JD, Tillitt DE, Zajicek JL, Brown SB (2005) Development of thiamine deficiencies and early mortality syndrome in lake trout by feeding experimental and feral fish diets containing thiaminase. J Aquat Anim Health 17:4–12. doi:10.1577/H03-078.1

    Article  Google Scholar 

  • Huang JW, Feng L, Liu Y, Jiang J, Jiang WD, Hu K, Li SH, Zhou X (2010) Effect of dietary thiamin supplementation on growth, body composition and intestinal enzyme activities of juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Nutr 17:233–240. doi:10.1111/j.1365-2095.2010.00756.x

    Article  Google Scholar 

  • Hung S, Deng D (2002) Sturgeon, Acipenser spp. In: Webster CD, Lim C (eds) Nutrient requirement and feeding of finfish for aquaculture. Kluwer Academic, New York, pp 344–357

    Chapter  Google Scholar 

  • Hurvitz A, Jackson K, Degani G, Levavi-Sivan B (2007) Use of endoscopy for gender and ovarian stage determinations in Russian sturgeon (Acipenser gueldenstaedtii) grown in aquaculture. Aquaculture 270:158–166. doi:10.1016/j.aquaculture.2007.05.020

    Article  Google Scholar 

  • IUCN (2012) IUCN Red List of Threatened Species. Available at: www.iucnredlist.org. Downloaded on 12 Nov 2012

  • Izquierdo MS, Fernandez-Palacios H, Tacon AGJ (2001) Effect of broodstock nutrition on reproductive performance of fish. Aquaculture 197:25–42. doi:10.1016/S0044-8486(01)00581-6

    Article  Google Scholar 

  • Jennings MJ, Philipp DP (1992) Reproductive investment and somatic growth rates in longear sunfish. Environ Biol Fish 35:257–271. doi:10.1007/BF00001892

    Article  Google Scholar 

  • Ketola HG, Bowser PR, Wooster GA, Wedge LR, Hurst SS (2000) Effects of thiamine on reproduction of Atlantic salmon and a new hypothesis for their extirpation in Lake Ontario. Trans Am Fish Soc 129:607–612. doi:10.1577/1548-8659(2000)129<0607:EOTORO>2.0.CO;2

    Article  CAS  Google Scholar 

  • Ketola HG, Isaacs GR, Robins JS, Loyd RC (2008) Effectiveness and retention of thiamine and its analogs administered to steelhead and landlocked Atlantic salmon. J Aquat Anim Health 20:29–38. doi:10.1577/H07-012.1

    Article  PubMed  Google Scholar 

  • Koski P, Bäckman C, Pelkonen O (2005) Pharmacokinetics of thiamine in female Baltic salmon (Salmo salar L.) broodfish. Environ Toxicol Pharmacol 19:139–152. doi:10.1016/j.etap.2004.06.001

    Article  CAS  PubMed  Google Scholar 

  • Lahnsteiner B, Berger F, Horvath A, Urbanyi B (2004) Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L. Aquac Res 35:519–528. doi:10.1111/j.1365-2109.2004.01034.x

    Article  Google Scholar 

  • Lee KJ, Dabrowski K (2004) Long-term effects and interactions of dietary vitamins C and E on growth and reproduction of yellow perch, Perca flavescens. Aquaculture 230:377–389. doi:10.1016/S0044-8486(03)00421-6

    Article  CAS  Google Scholar 

  • Lee B, Jaroszewska M, Dabrowski K, Czesny S, Rinchard J (2009) Effects of vitamin B1 (thiamine hydrochloride) deficiency in lake trout alevins and preventive treatments. J Aquat Anim Health 21:290–301. doi:10.1577/H07-053.1

    Article  PubMed  Google Scholar 

  • Lim C, LeaMaster B (1991) Thiamin requirement of red hybrid tilapia grown in seawater. In: 22nd annual conference and exposition, world aquaculture society, San Juan, Puert Rico, June 16–20

  • Lim C, Yildirim-Aksoy M (2011) Thiamin requirement of Nile tilapia, Oreochromis niloticus. J World Aquac Soc 42:824–833. doi:10.1111/j.1749-7345.2011.00531.x

    Article  Google Scholar 

  • Lim C, Klesius PH, Li MH, Robinson EH (2000) Interaction between dietary levels of iron and vitamin C on growth, hematology, immune response and resistance of channel catfish (Ictalurus punctatus) to Edwardsilla ictaluri challenge. Aquaculture 185:313–327. doi:10.1016/S0044-8486(99)00352-X

    Article  CAS  Google Scholar 

  • Mancinelli R, Ceccanti M, Guiducci MS, Sasso GF, Sebastiani G, Attilia ML, Allen JP (2003) Simultaneous liquid chromatographic assessment of thiamine, thiamine monophosphate and thiamine diphosphate in human erythrocytes: a study on alcoholics. J Chromatogr 789:355–363. doi:10.1016/S1570-0232(03)00139-9

    CAS  Google Scholar 

  • Masumoto T, Hardy RW, Casillas E (1987) Comparison of transketolase activity and thiamin pyrophosphate levels in erythrocytes and liver of rainbow trout (Salmo gairdneri) as indicator of thiamin status. J Nutr 117:1422–1426. doi:10.1007/BF098690209

    CAS  PubMed  Google Scholar 

  • Menezes JC, Tavares-Dias M, Akifumi E, Andrade JA, Brasil LM, Roubach R, Urbinati EC, Marcon JL, Affonso AJ (2006) The influence of dietary vitamin C and E supplementation on the physiological response of pirarucu, Arapaima gigas, in net culture. Comp Biochem Physiol 145A:274–279. doi:10.1016/j.cbpa.2006.03.017

    Article  Google Scholar 

  • Moltschaniwskyj NA (1995) Multiple spawning in the tropical squid Photololigo sp: what is the cost in somatic growth? Mar Biol 124:127–135. doi:10.1007/BF00349154

    Article  Google Scholar 

  • Moreau R, Dabrowski K, Sato PH (1999) Renal L-gulono-1,4-lactone oxidase as affected by dietary ascorbic acid in lake sturgeon (Acipenser fulvescens R). Aquaculture 180:359–372. doi:10.1016/S0044-8486(99)00211-2

    Article  CAS  Google Scholar 

  • Morito CLH, Conrad DH, Hilton JW (1986) The thiamin deficiency signs and requirement of rainbow trout (Salmo gairdneri, Richardson). Fish Physiol Biochem 1:93–104. doi:10.1007/BF02290209

    Article  CAS  PubMed  Google Scholar 

  • NRC (1993) Nutrient requirements of fish. National Academies Press, Washington DC, 114 pp

  • Nyina-wamwiza L, Defreyne PS, Ngendahayo L, Milla S, Mandiki SNM, Kestemont P (2012) Effects of partial or total fish meal replacement by agricultural by-product diets on gonad maturation, sex steroids and vitellogenin dynamics of African catfish (Clarias gariepinus). Fish Physiol Biochem 38:1287–1298. doi:10.1007/s10695-012-9616-2

    Article  CAS  PubMed  Google Scholar 

  • Piros B, Glogowski J, Kolman R, Rzemieniecki A, Domagala J, Horvath A, Urbanyi B, Ciereszko A (2002) Biochemical characterization of Siberian sturgeon Acipenser baeri and sterlet Acipenser ruthenus milt plasma and spermatozoa. Fish Physiol Biochem 26:289–295. doi:10.1023/A:1026280218957

    Article  CAS  Google Scholar 

  • Pourkazemi M, Skibinski DOF, Beardmore JA (1999) Application of mtDNA d-loop region for the study of Russian sturgeon population structure from Iranian coastline of the Caspian Sea. J Appl Ichthyol 15:23–28. doi:10.1111/j.1439-0426.1999.tb00199.x

    Article  CAS  Google Scholar 

  • Quince C, Shuter BJ, Abrams PA, Lester NP (2008) Biphasic growth in fish II: empirical assessment. J Theor Biol 27:207–214. doi:10.1016/j.jtbi.2008.05.030

    Article  Google Scholar 

  • Rey Vazquez G, Guerrero GA (2007) Characterization of blood cells and hematological parameters in Cichlasoma dimerus (Teleostei, Perciformes). Tissue Cell 39:151–160

    Article  CAS  PubMed  Google Scholar 

  • Rijnsdorp AJ (1990) The mechanism of energy allocation over reproduction and somatic growth in female North Sea plaice, Pleuronectes platessa L. Neth J Sea Res 25:279–289. doi:10.1016/0077-7579(90)90027-E

    Article  Google Scholar 

  • Rinchard J, Dabrowski K, Marschal E (2011) Egg thiamine concentration affects embryo survival in Lake Erie walleye. Environ Biol Fish 90:53–60. doi:10.1007/s10641-010-9717-7

    Article  Google Scholar 

  • Sandnes K (1991) Vitamin C in fish nutrition. Fisk Skr Ser Ernaer 4:3–32

    CAS  Google Scholar 

  • Sokolov LI, Vasiliev VP (1989) Acipenser ruthenus Linnaeus. In: Holčik J (ed) The freshwater fishes of Europe. AULA-Verlag, Wiesbaden, pp 227–262

    Google Scholar 

  • Tatina M, Bahmani M, Soltani M, Abtahi B, Gharibkhani M (2010) Effects of different levels of dietary vitamin C and E on some of hematological and biochemical parameters of sterlet (Acipenser ruthenus). J Fish Aquat Sci 5:1–11. doi:10.3923/jfas.2010.1.11

    Article  CAS  Google Scholar 

  • Watanabe T (1985) Importance of the study of broodstock nutrition for further development of aquaculture. In: Cowey CB, Mackie AM, Bell JG (eds) Nutrition and feeding in fish. Academic Press, London, pp 395–414

    Google Scholar 

  • Watanabe T, Vassallo-Agius R (2003) Broodstock nutrition research on marine finfish in Japan. Aquaculture 227:35–61. doi:10.1016/S0044-8486(03)00494-0

    Article  CAS  Google Scholar 

  • Werner MR, Rook B, Greil R (2006) Egg-thiamine status and occurrence of early mortality syndrome (EMS) in Atlantic salmon from the St. Marys River, Michigan. J Gt Lakes Res 32:293–305. doi:10.3394/0380-1330(2006)32[293:ESAOOE]2.0.CO;2

    Google Scholar 

  • Williot P, Bruna TR, Rouaulta T, Pelarda M, Merciera D, Ludwigb A (2005) Artificial spawning in cultured sterlet sturgeon, Acipenser ruthenus L., with special emphasis on hermaphrodites. Aquaculture 246:263–273. doi:10.1016/j.aquaculture.2005.02.048

    Article  Google Scholar 

  • Yuan CT, Pan XP, Gong Y, Xia AJ, Wu GH, Tang JQ, Han XD (2008) Effects of astragalus polysaccharides (APS) on the expression of immune response genes in head kidney, gill and spleen of the common carp, Cyprinus carpio L. Int Immunopharmacol 8:51–58. doi:10.1016/j.intimp.2007.10.009

    Article  CAS  PubMed  Google Scholar 

  • Yue Z (2011) Husbandry and dietary effects on sturgeon (Acipenser transmontanus) farmed for caviar. MSc thesis in Animal Biology, University of California, Davis, 48 pp

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Acknowledgments

We would like to thank the staffs of Shahid Dr. Beheshti Sturgeon Fish Propagation and Rearing Complex for providing the sterlet broodstocks and necessary facilities for conducting this experiment. We are also grateful to all colleagues that supported us with their kind helps during this study.

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Authors and Affiliations

  1. Fisheries Department, Faculty of Natural Resources, University of Guilan, 1144, Sowmeh Sara, Guilan, Iran

    Sareh Ghiasi & Bahram Falahatkar

  2. School of Environment and Natural Resources, The Ohio State University, Columbus, OH, 43210, USA

    Konrad Dabrowski

  3. Shahid Dr. Beheshti Sturgeon Fish Propagation and Rearing Complex, Rasht, Guilan, Iran

    Alireza Abasalizadeh

  4. Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, Turkey

    Murat Arslan

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  1. Sareh Ghiasi
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Correspondence to Bahram Falahatkar.

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Ghiasi, S., Falahatkar, B., Dabrowski, K. et al. Effect of thiamine injection on growth performance, hematology and germinal vesicle migration in sterlet sturgeon Acipenser ruthenus L.. Aquacult Int 22, 1563–1576 (2014). https://doi.org/10.1007/s10499-014-9765-7

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