Abstract
The objective of the present work was to evaluate the effects of three different rates of long chain n-3 polyunsaturated fatty acid (LC n-3 PUFA) on the sperm number of Astacus leptodactylus. The experiment was carried out with the four following treatments: control group (not supplemented LC n-3 PUFA), D1 (1 %), D2 (2 %) and D3 (3 %). The sperm number of A. leptodactylus was counted; the reproductive system was weighted; and the macroscopical analysis of the testes and vasa deferentia (DVD) were conducted. In addition, gonado-somatic index (GI) and testes index (TI) were also calculated. The results showed that there was a significant difference between the crayfish fed LC n-3 PUFA supplemented diets (D1, D2, D3) and control in mean sperm number (P < 0.05). The highest sperm number was found for the crayfish fed D3 (6.27 ± 0.14 × 108 sperm/distal vas deferens section). The results also showed that the reproductive system of the crayfish fed D3 and D2 was significantly heavier than that of the crayfish fed the control and D1 (P < 0.05). On the other hand, there was not a significant difference in the testes weight and vasa deferentia weight between the crayfish fed the control and D1, and between the crayfish fed D2 and D3 (P < 0.05). GI of the crayfish fed D3 and D2 was significantly higher than that of the crayfish fed control and D1 (P > 0.05), but there was not a significant difference in the TI of the crayfish fed control, D1, D2 and D3 (P > 0.05). In conclusion, diets containing different levels of LC n-3 PUFA significantly increase the sperm number of A. leptodactylus, and they are crucial for the production of higher number of sperm in this species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alfaro J, Lozano X (1993) Development and deterioration of spermatophores in pond-reared Penaeus vannamei. J World Aquac Soc 24:522–529
American Public Health Association (APHA) (1985) Standard methods for the examination of water and wastewater, 16th edn. Washington, DC
Association of Official Analytical Chemists (AOAC) (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists., Washington, DC
Bell MV, Dickand JR, Buda CS (1997) Molecular speciation of fish spermphospholipids: large amounts of dipolyunsaturated phosphatidylserine. Lipids 32:1085–1091
Braga AL, Nakayama CL, Martins JG, Colares EP, Wasielesky W Jr (2010) Spermatophore quality of the pink shrimp Farfantepenaeus paulensis (Decapoda, Dendrobranchiata) broodstock fed with different maturation diets. Aquaculture 307:44–48
Bray WA, Lawrence AL (1992) Reproduction of Penaeus species in captivity. In: Fast A, Lester LJ (eds) Marine shrimp culture: principles and practices. Elsevier, The Netherlands, pp 93–169
Browdy CL (1992) A review of the reproductive biology of Penaeus species: perspectives on controlled shrimp maturation systems for high quality nauplii production. In: J Wyban (ed) Proceedings of the special session on shrimp farming. World Aquaculture Society, USA, Baton Rouge, pp 22–51
Bugnot AB, López Greco LS (2009a) Sperm production in the red claw crayfish Cherax quadricarinatus (Decapoda, Parastacidae). Aquaculture 295:292–299
Bugnot AB, López Greco LS (2009b) Structural alterations in the male reproductive system of the freshwater crayfish, Cherax quadricarinatus (Decapoda, Parastacidae). J Invertebr Pathol 102:160–166
Ceballos Vázquez BP, Rosas C, Racotta IS (2003) Sperm quality in relation to age a weight of white shrimp Litopenaeus vannamei. Aquaculture 228:141–151
Cerda J, Carrillo M, Zanuy S, Ramos J (1994) Effect of food ration on estrogen and vitellogenin plasma levels, fecundity and larval survival in captive sea bass, Dicentrarchus labrax: preliminary observations. Aquat Living Resour 7:255–256
Coman GJ, Arnold SJ, Callaghan TR, Preston NP (2007) Effect of two maturation diet combinations on reproductive performance of domesticated Penaeus monodon. Aquaculture 263:75–83
Díaz AC, Fernández Gimenez AV, Harán N, Fenucci JL (2001) Reproductive performance of male red shrimp Pleoticus muelleri Bate (Decapoda, Penaeoidea) in culture conditions. J World Aquac Soc 32:236–242
Erkan M, Tunalı Y, Sancar-Bas S (2009) Male reproductive system morphology and spermatophore formation in Astacus leptodactylus (Eschscholtz, 1823) (Decapoda: Astacidae). J Crustac Biol 29(1):42–50
Fernández-Palacios H, Izquierdo MS, Robaina L, Valencia A, Salhi M, Vergara J (1995) Effect of n-3 HUFA level in broodstock diets on egg quality of gilthead seabream (Sparus aurata L.). Aquaculture 132:325–337
Grubert MA, Dunstan GA, Ritar AJ (2004) Lipid and fatty acid composition of pre-and post-spawning blacklip (Haliotis rubra) and greenlip (Haliotis laevigata) abalone conditioned at two temperatures on a formulated feed. Aquaculture 242:297–311
Harlıoğlu MM, Köprücü K, Özdemir Y (2002) The effect of vitamin E on the pleopodal egg number of Astacus leptodactylus (Eschscholtz, 1823). Aquac Int 10:391–397
Harlıoğlu MM, Barım Ö, Türkgülü İ, Harlıoğlu AG (2004) The potential fecundity of an introduced population of freshwater crayfish, Astacus leptodactylus leptodactylus (Eschscholtz, 1823). Aquaculture 230:189–195
Holdich DM (2002) General biology-background and functional morphology. In: Holdich DM (ed) Biology of freshwater crayfish. Blackwell, Oxford, pp 3–30
Izquierdo MS, Fernández-Palacios H, Tacon AGJ (2001) Effect of broodstock nutrition on reproductive performance of fish. Aquaculture 197:25–42
Krol J, Glogowski J, Demska-Zakes K, Hliwa P (2006) Quality of semen and histological analysis of testes in Eurasian perch Perca fluviatilis L. during a spawning period. Czech J Anim Sci 5:220–226
Lahnsteiner F, Patzner RA, Weismann T (1994) The testicular main ducts and spermatic ducts in cyprinid fishes II. Composition of the seminal fluid. J Fish Biol 44:459–467
Lenzi A, Picardo M, Gandini L, Dondero F (1996) Lipids of sperm plasma membrane: from polyunsaturated fatty acid considered as markers of sperm function to possible scavenger therapy. Hum Reprod Update 2:246–256
Leung-Trujillo JR, Lawrence AL (1987) Observations on the decline effect in sperm quality of Penaeus setiferus under laboratory conditions. Aquaculture 65:363–370
López Greco LS, Lo Nostro FL (2008) Structural changes of the spermatophore in the freshwater “red claw” crayfish Cherax quadricarinatus (von Martens, 1898) (Decapoda, Parastacidae). Acta Zool 89:149–155
López Greco LS, Vázquez FJ, Rodríguez E (2007) Morphology of the male reproductive system and spermatophore formation in the freshwater “red claw” crayfish Cherax quadricarinatus (von Martens, 1898) (Decapoda, Parastacidae). Acta Zool 88:223–229
Meunpol O, Meejing P, Piyatiratitivorakul S (2005) Maturation diet based on fatty acid content for male Penaeus monodon (Fabricius) broodstock. Aquac Res 36:1216–1225
National Research Council (NRC) (1987) Predicting feed intake of food-producing animals. Subcommittee on Feed Intake Committee on Animal Nutrition Board on Aqriculture. National Academy Press, Washington, D.C
Peixoto S, Cavalli RO, Wasielesky W, D’Incao F, Krummenauer D, Milach ÂM (2004) Effects of age and size on reproductive performance of captive Farfantepenaeus paulensis broodstock. Aquaculture 238:173–182
Perez Velazquez M, Bray WA, Lawrence AL, Gatlin DM III, González-Félix ML (2001) Effect of temperature on sperm quality of captive Litopenaeus vannamei broodstock. Aquaculture 198:209–218
Pratoomchat B, Piyatiratiticorakul S, Menasveta P (1993) Sperm quality of pond-reared and wild-caught Penaeus monodon in Thailand. J World Aquac Soc 24:530–541
Reigh RC, Braden SL, Craig RJ (1990) Apparent digestibility coefficients for common feedstuffs in formulated diets for red swamp crayfish, Procambarus clarkii. Aquac Soc 84:321–334
Sargent JR (1995) Origin and functions of egg lipids: nutritional implications. In: Bromage NR, Roberts RJ (eds) Broodstock management and eggs and larval quality. Blackwell, London, pp 353–372
Sorensen PW, Hara TJ, Stacey NE, Goetz FW (1988) F prostaglandins function as potent stimulants that comprise the post-ovulatory female sex pheromone in goldfish. Biol Reprod 39:1039–1050
Stacey NE, Goetz FW (1982) Role of prostaglandins in fish reproduction. Can J Fish Aquat Sci 39:92–98
Van Harreveld A (1936) A physiological solution for freshwater crustaceans. Proc Soc Exp Biol Med 34:428–432
Vassallo-Agius R, Watanabe T, Yoshizaki G, Satoh S, Takeuchi Y (2001) Quality of eggs and spermatozoa of rainbow trout fed an n-3 essential fatty acid-deficient diet and its effects on the lipid and fatty acid components of eggs, semen and livers. Fish Sci 67:818–827
Wade MG, Van der Kraak G, Gerrits MF, Ballantyne JS (1994) Release and steroidogenic actions of polyunsaturated fatty acids in the goldfish testis. Biol Reprod 51:131–139
Watanabe T, Takeuchi T, Saito M, Nishimura K (1984) Effect of low protein-high calorie or essential fatty acid deficiency diet on reproduction of rainbow trout. Nippon Suisan Gakkaishi 50(7):1207–1215
Wouters R, Lavens P, Nieto J, Sorgeloos P (2001) Penaeid shrimp broodstock nutrition: an updated review on research and development. Aquaculture 202:1–21
Acknowledgments
This study was carried out as a part of a research project, “Increasing reproductive efficiency of freshwater crayfish (Astacus leptodactylus Eschscholtz)” supported by the Scientific and Technological Research Council of TURKEY (Project No: TÜBİTAK-TOVAG 110O418).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Harlioğlu, M.M., Köprücü, K., Harlioğlu, A.G. et al. Effect of dietary n-3 series fatty acids on sperm production in the freshwater crayfish, Astacus leptodactylus (Eschscholtz) (Astacidae). Aquacult Int 21, 273–282 (2013). https://doi.org/10.1007/s10499-012-9549-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10499-012-9549-x