Abstract
Cardinium have been found as endosymbionts of Brevipalpus phoenicis, the mite vector of the Citrus leprosis virus. With the long-term objective being to understand the mechanisms of plant-virus-vector interactions, we evaluated the different storage conditions and periods, as well as the number of mites needed for PCR-amplification of such endosymbionts, making it possible to collect mites in different geographical regions without prolonged storage compromising subsequent analyses.
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Acknowledgements
The authors gratefully acknowledge the financial support of FAPESP (04/10511-3), CNPq (507085/2004-3), and USDA, APHIS, CPHST Project E3M01 through USDA, ARS Specific cooperative agreement No. 58-6618-3-F140. VMN has a FAPESP fellowship (04/11854-1).
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Novelli, V.M., Freitas-Astúa, J., Arrivabem, F. et al. Effects of temperature, storage period and the number of individuals on the detection of the false spider mite Cardinium endosymbiont. Exp Appl Acarol 42, 17–21 (2007). https://doi.org/10.1007/s10493-007-9070-5
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DOI: https://doi.org/10.1007/s10493-007-9070-5