Abstract
“Psychrodesulfovibrio”, a proposed genus within the family Desulfovibrionaceae, is a group of sulfate-reducing bacteria with biogeochemical significance but restricted child taxa availability. In this study, a strictly anaerobic bacterium, designed strain FT415T, was isolated from mangrove sediments in Futian Mangrove Nature Reserve in Shenzhen, China. The strain was Gram-stain-negative, motile, and vibrio-shaped with a single polar flagellum, which grew at the temperature range of 15–42 °C (optimum 37 °C), pH range of 6.0–7.5 (optimum 6.8), and in the presence of 0–36 g l−1 NaCl (optimum 6 g l−1 NaCl). In the presence of sulfate, electron donors including lactate, ethanol, pyruvate, malate, fumarate, succinate, cysteine, and glycerol were incompletely oxidized to acetate, and H2 and formate were used as electron donors with acetate as the carbon source by strain FT415T. Sulfate, thiosulfate, sulfide, and anthraquinone-2,6-disulfonate were reduced in the presence of lactate. Fe(III) oxide was reduced without cell growth. Fermentative growth was observed with pyruvate and cysteine. Vitamins were not required for growth. The major cellular fatty acids (> 10%) were C16:0, summed feature 10 (C18:1 c11/t9/t6 and/or unknown ECL 17.834), C16:1 cis 9, and C18:0. The major polar lipids were phosphatidylethanolamine, phospholipids, and aminolipids. The predominant menaquinone was MK-6(H2). The genomic DNA G+C content was 56.7%. Phylogenetic analysis showed that strain FT415T shared a 98.1% similarity in 16S rRNA gene sequence, an average nucleotide identity value of 84.0%, an average amino-acid identity value of 85.4%, and a digital DNA-DNA hybridization value of 25.7% with its closest relative Desulfovibrio subterraneus HN2T, which has been proposed to be transferred to the genus “Psychrodesulfovibrio”. Based on phenotypic, phylogenetic, and genotypic evidence, a new species of the family Desulfovibrionaceae, Desulfovibrio mangrovi sp. nov. was proposed with the type strain FT415T (=GDMCC 1.3410T=KCTC 25525T).
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Data availability
The 16S rRNA gene and genome sequences from the strain FT415T are available in NCBI under the accession numbers: ON970160 and CP104208, respectively. The genome sequence is additionally available in IMG with genome ID 2952831780.
Abbreviations
- SRB:
-
Sulfate-reducing bacteria
- ANI:
-
Average nucleotide identity
- AAI:
-
Average amino acid identity
- dDDH:
-
Digital DNA-DNA hybridization
- UBCG:
-
Up-to-date bacterial core gene
- TEM:
-
Transmission electron microscopy
- AQDS:
-
Anthraquinone-2,6-disulfonate
- DMSO:
-
Dimethyl sulfoxide
- OD:
-
Optical density
- MES:
-
2-(N-Morpholine) ethanesulfonic acid
- HEPES:
-
N-2-Hydroxyethylpiperazine-N’-2-ethanesulfonic acid
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Acknowledgements
We thank the Instrument Analysis Center of Shenzhen University for the assistance with collection of transmission electron microscopy images.
Funding
This research was supported by the National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700), the National Natural Science Foundation of China (42207144, 32225003, 31970105, 92251306, 32070108), the China Postdoctoral Science Foundation (2021TQ0212), the Innovation Team Project of Universities in Guangdong Province (2020KCXTD023), and the Shenzhen Science and Technology Program (JCYJ20200109105010363, KCXFZ20201221173404012).
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JZ and ML designed the research. JZ isolated the strain, performed the research, analyzed the data, and wrote the manuscript. CJZ and ML offered critical revisions of the manuscript. All authors contributed to the article and approved the submitted version.
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Zhou, J., Zhang, CJ. & Li, M. Desulfovibrio mangrovi sp. nov., a sulfate-reducing bacterium isolated from mangrove sediments: a member of the proposed genus “Psychrodesulfovibrio”. Antonie van Leeuwenhoek 116, 499–510 (2023). https://doi.org/10.1007/s10482-023-01820-5
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DOI: https://doi.org/10.1007/s10482-023-01820-5