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Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade postoperative infection

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Abstract

We present a post-operative infection caused by a methicillin-resistant Staphylococcus aureus strain, previously isolated in the preoperative screening, in a patient submitted to femoral osteosynthesis, successfully treated with oral ciprofloxacin. The isolate exhibited in vitro resistance to ciprofloxacin, Staphylococcal Cassette Chromosome mec type IV, it was negative for the lukS-PV Panton–Valentine leucocidin gene and belonged to ST2594 in multilocus sequence typing analysis. Whole genome sequencing revealed a genome size of 2,818,289 base pairs. The annotated genomes of ST2594 and N315 strains were compared, looking for genes related to virulence and resistance. The lack of the tst, sec, sel genes, associated with a mutation in the clfA gene, may partially explain the low morbity in this case.

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Funding

Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq.).

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Authors and Affiliations

  1. Department of Microbiology, Immunology and Parasitology, Medical Science School, State University of Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 3º andar, Rio de Janeiro, RJ, CEP: 20551-030, Brazil

    Leonardo Ribeiro Bastos, Elizabeth Andrade Marques & Robson Souza Leão

  2. Institute of Bioscience, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Maria Clara Fernandes Martins

  3. Biochemistry Department, Institute of Biology Roberto Alcântara Gomes, State University of Rio de Janeiro, Rio de Janeiro, Brazil

    Rodolpho Mattos Albano

Authors
  1. Leonardo Ribeiro Bastos
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  2. Maria Clara Fernandes Martins
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  3. Rodolpho Mattos Albano
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  4. Elizabeth Andrade Marques
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  5. Robson Souza Leão
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Corresponding author

Correspondence to Robson Souza Leão.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institutional research committee (CAAE: 39070314.0.0000.5256).

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Informed consent was obtained from the participant included in the study.

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Bastos, L.R., Martins, M.C.F., Albano, R.M. et al. Whole genome sequencing of a ST2594 MRSA strain causing non-mucosal preoperative colonization and low-grade postoperative infection. Antonie van Leeuwenhoek 112, 961–964 (2019). https://doi.org/10.1007/s10482-019-01229-z

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  • DOI: https://doi.org/10.1007/s10482-019-01229-z

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