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A protein complex bearing an oxidase with napthalene dihydrodiol dehydrogenase activity is induced in Mucor circinelloides strain YR-1 during growth on polycyclic aromatic compounds

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Abstract

Fungi are organisms capable of growing in a myriad of conditions and respond to counteract environmental cues. Several locations in the world are polluted with oil and its derivatives, and some microorganisms tolerant to these compounds have been isolated. Some fungi can grow in the presence of molecules such as polycyclic aromatic hydrocarbons as sole carbon sources. In this report, we further characterized the induced enzymes with phenanthrene from Mucor circinelloides YR-1 strain, isolated from a polluted field near a petrochemical facility in México. We identified a putative oxidase that is induced when growth with phenanthrene as sole carbon source at a pH of 8.5 and is NADP+ dependent. We show that this enzyme bears naphthalene dihydrodiol dehydrogenase activity with substrate preference for the cis-naphthalene over the trans-naphthalene, with an optimal pH in the range of 8–10. Mass spectrometry analysis revealed that the induced enzyme belongs to the NADP+ oxidase family enzymes with the typical Rossmann-fold for NADP+ binding. This enzyme seems to form a high molecular weight structure (~ 541 kDa) and with a monomer of 57 kDa, suggesting that the multimer is constituted of 10 subunits. Our findings contribute to understanding of the roles that dihydrodiol dehydrogenases have in organisms exposed to toxic compounds in the environment and can regulate their expression.

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Acknowledgements

Authors are grateful for the support by CONACyT/CIBIOGEM Grant Number 264456. A grant from DAIP/Guanajuato University in the Convocatoria Institucional de Investigación Científica 2016–2017. Apoyo institucional para fortalecer la excelencia académica convenio 89/2016. Ángeles Rangel Serrano and Tannia Razo Soria for their skillful technical assistance.

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  1. Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, 36050, Guanajuato, GTO, Mexico

    Jazmín Areli Álvarez Copado, Roberto Zazueta Sandoval, Areli Durón Castellanos, Felipe Padilla-Vaca & Bernardo Franco

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  1. Jazmín Areli Álvarez Copado
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  2. Roberto Zazueta Sandoval
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  3. Areli Durón Castellanos
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  4. Felipe Padilla-Vaca
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Correspondence to Bernardo Franco.

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Roberto Zazueta Sandoval—Retired.

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Copado, J.A.Á., Sandoval, R.Z., Castellanos, A.D. et al. A protein complex bearing an oxidase with napthalene dihydrodiol dehydrogenase activity is induced in Mucor circinelloides strain YR-1 during growth on polycyclic aromatic compounds. Antonie van Leeuwenhoek 111, 297–309 (2018). https://doi.org/10.1007/s10482-017-0950-3

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