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Idiomarina andamanensis sp. nov., an alkalitolerant bacterium isolated from Andaman Sea water

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Abstract

Two closely related aerobic, Gram-negative rod shaped bacteria (strain W5T and W3) were isolated from Andaman Sea. Heterotrophic growth on marine agar was observed at 15–45 °C and pH 6–10. Strain W5T showed maximum 16S rRNA sequence similarity of 99.58% with Idiomarina marina JCM 15083T. DNA fingerprinting analysis by ERIC-REP PCR, PFGE and MLSA revealed differences in banding patterns, also DNA–DNA hybridization values were well below 70% confirming W5T to be a new species. DNA G+C content was 46.7 mol%. Major fatty acids were iso-C15:0, iso-C17:0, iso-C17:1 ω9c, iso-C13:0 3OH, iso-C11:0 3OH and C16:0. Polar lipids included phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) diphosphatidylglycerol (DPG) phospholipid (PL) two aminolipids (AL) and two unidentified lipids (L1-2). Q-8 is the predominant ubiquinone. On the basis of polyphasic taxonomic study, strain W5T is considered to be representative of a new species of the genus Idiomarina, for which the name Idiomarina andamanensis sp. nov. is being proposed. The type strain W5T (= LMG 29773T = JCM 31645T) was isolated from Andaman Sea.

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Acknowledgements

We are thankful to Prof. J. P Euzeby (Ecole Nationale Veterinaire, France) for etymological advice. We are grateful to the Ministry of Environment and Forest, Wild Life Division, Government of India for allowing us to collect water sample from Andaman Sea. Author SZ acknowledge the Department of Biotechnology, Government of India, for providing fellowship. This work was supported in part by the funding received from Department of Biotechnology, Government of India (D.O No. BT/PR7661/AAQ/3/629/2013) to SKD.

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  1. Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751023, India

    Sherin Zachariah & Subrata K. Das

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  1. Sherin Zachariah
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  2. Subrata K. Das
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Correspondence to Subrata K. Das.

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Zachariah, S., Das, S.K. Idiomarina andamanensis sp. nov., an alkalitolerant bacterium isolated from Andaman Sea water. Antonie van Leeuwenhoek 110, 1581–1592 (2017). https://doi.org/10.1007/s10482-017-0908-5

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