Abstract
A Gram-positive, non-motile and coccoid strain, designated XY-FW106T, was isolated from a marine flatworm identified to be Planocera sp. The 16S rRNA gene sequence of this pink organism was consistent with membership of the genus Deinococcus, with high sequence similarity to Deinococcus aetherius ST0316T (94.7%). The optimum growth temperature range of the strain XY-FW106T was found to be 25–30 °C and optimum growth occurs at pH 7.2–7.4 without NaCl. The strain XY-FW106T was found to contain unidentified glycolipids, unidentified phosphoglycolipids, unidentified phospholipids and unidentified lipids, which differed from those of closely related species. Menaquinone MK-8 was identified as the major respiratory quinone and the predominant cellular fatty acids were found to be Summed Feature 3 (C16:1 ω7c/C16:1 ω6c), C16:0, iso-C15:0, and Summed Feature 8 (C18:1 ω7c/C18:1 ω6c). The DNA G+C content was determined to be 70.2 mol%. The biochemical and chemotaxonomic data together suggest that the strain represents a new species for which the name Deinococcus planocerae sp. nov. is proposed. The type strain is XY-FW106T (=MCCC 1K01499T=KCTC 33809T).
Similar content being viewed by others
Abbreviations
- KCTC:
-
Korean Collection for Type Cultures
- MCCC:
-
Marine Culture Collection of China
References
Brooks BW, Murray RGE (1981) Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int J Syst Bacteriol 31:353–360
Chen W, Wang B, Hong H, Yang H, Liu SJ (2012) Deinococcus reticulitermitis sp. nov., isolated from a termite gut. Int J Syst Evol Microbiol 62:78–83
Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354
de Groot A, Chapon V, Servant P, Christen R, Fischer-Le Saux M, Sommer S, Heulin T (2005) Deinococcus deserti sp. nov., a gammaradiation-tolerant bacterium isolated from the Sahara Desert. Int J Syst Evol Microbiol 55:2441–2446
Dong X, Cai M (2001) Determinative manual for routine bacteriology. Scientific Press, Beijing
Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376
Ferreira AC, Nobre MF, Rainey FA, Silva MT, Wait R, Burghardt J, Chung AP, da Costa MS (1997) Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947
Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. Washington, DC, American Society for Microbiology
Hirsch P, Gallikowski CA, Siebert J, Peissl K, Kroppenstedt R, Schumann P, Stackebrandt E, Anderson R (2004) Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27:636–645
Kates M (1986) Techniques of lipidology, 2nd ed. rev. Elsevier, Amsterdam, pp 106–107, 241–246
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH et al (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Lai W-A, Kämpfer P, Arun AB, Shen F-T, Huber B, Rekha PD, Young C-C (2006) Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int J Syst Evol Microbiol 56:787–791
Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acids techniques in bacterial systematics. Wiley, Chichester, pp 115–175
Mesbah M, Whitman WB (1989) Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479: 297–306
Rainey FA, Ray K, Ferreira M, Gatz BZ, Nobre MF, Bagaley D, Rash BA, Park MJ, Earl AM et al (2005) Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl Environ Microbiol 71:5225–5235
Rzhetsky A, Nei M (1992) Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35:367–375
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc., Newark
Srinivasan S, Lee J-J, Lim S, Joe M, Kim MK (2012) Deinococcus humi sp. nov., isolated from soil. Int J Syst Evol Microbiol 62:2844–2850
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Wang W, Mao J, Zhang Z-D, Tang Q-Y, Xie Y-Q, Zhu J, Zhang L-X, Liu Z-H, Shi Y-H, Goodfellow M (2010) Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil. Int J Syst Evol Microbiol 60:2006–2010
Xu Y, Li Q, Tian R, Lai Q, Zhang Y (2015) Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm. Antonie Van Leeuwenhoek 108:127–132
Yang Y, Itoh T, Yokobori S, Itahashi S, Shimada H, Satoh K, Ohba H, Narumi I, Yamagishi A (2009) Deinococcus aerius sp. nov., isolated from the high atmosphere. Int J Syst Evol Microbiol 59:1862–1866
Yang Y, Itoh T, Yokobori S-I, Shimada H, Itahashi S, Satoh K, Ohba H, Narumi I, Yamagishi A (2010) Deinococcus aethrius sp. nov., isolated from the stratosphere. Int J Syst Evol Microbiol 60:776–779
Zhang Y-Q, Sun C-H, Li W-J, Yu L-Y, Zhou J-Q, Zhang Y-Q, Xu L-H, Jiang C-L (2007) Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. Int J Syst Evol Microbiol 57:370–375
Acknowledgements
This work was financially supported by grants from the National Natural Science Foundation of China (No. 41576146) and Seed Funding from Scientific and Technical Innovation Council of Shenzhen Government (Nos. 827000041; 827000012).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Lin, H., Wang, Y., Huang, J. et al. Deinococcus planocerae sp. nov., isolated from a marine flatworm. Antonie van Leeuwenhoek 110, 811–817 (2017). https://doi.org/10.1007/s10482-017-0854-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-017-0854-2