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Geodermatophilus daqingensis sp. nov., isolated from petroleum-contaminated soil

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Abstract

A novel Gram-positive actinobacterium, designated WT-2-1T, was isolated from a sample of petroleum-contaminated soil collected in Daqing, Heilongjiang province, China and characterised using a polyphasic taxonomic approach. The optimal growth for strain WT-2-1T was found to be at 25–35 °C and at pH 6.0–9.0 and with 0–4% (w/v) NaCl, forming blackish green-coloured colonies. Chemotaxonomic and molecular characteristics of the isolate match those described for members of the genus Geodermatophilus. The peptidoglycan was found to contain meso-diaminopimelic acid; galactose, glucose and xylose were detected as diagnostic sugars. The main phospholipids were identified as diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and phosphatidylglycerol; MK-9(H4) was the dominant menaquinone present. The major cellular fatty acids were identified as iso-C16:0 and iso-C15:0. 16S rRNA gene sequence analysis showed that strain WT-2-1T is a member of the genus Geodermatophilus, with high sequence similarities to Geodermatophilus aquaeductus BMG801T (98.4%), Geodermatophilus saharensis CF5/5T (98.4%), Geodermatophilus bullaregiensis BMG841T (98.3%) and Geodermatophilus normandii CF5/3T (98.3%). Based on the phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, the isolate is concluded to represent a novel species of the genus Geodermatophilus, for which the name Geodermatophilus daqingensis sp. nov. is proposed. The type strain is WT-2-1T (=CGMCC 4.7381T = DSM 104001T).

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Acknowledgements

This work was supported by the National Natural Science Fund of China (31372351 and 31672469), the Youth Foundation of Daqing Normal University (14ZR17)and the National Natural Science Fund of Heilongjiang Province (QC2011C094).

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    Authors and Affiliations

    1. College of Resources and Environment, Northeast Agricultural University Harbin, Harbin, 150030, People’s Republic of China

      Yang Wang & Xiuhong Xu

    2. Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People’s Republic of China

      Liguo Zhang, Yuanling Zhao, Jianxin Liu, Cui Huang, Jing Wang & Yingying Hu

    3. College of Mathematics and Information Sciences of Guangxi University, Nanning, 530004, People’s Republic of China

      Xiaofei Zhang

    4. Daqing Normal University, Daqing, 163712, People’s Republic of China

      Yang Wang & Guoling Ren

    5. Department of Mathematics, Jiamusi University, Jiamusi, 154007, People’s Republic of China

      Jinying Huang & Yu Zhao

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    1. Yang Wang
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    Yang Wang and Liguo Zhang contributed equally to this work.

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    Wang, Y., Zhang, L., Zhang, X. et al. Geodermatophilus daqingensis sp. nov., isolated from petroleum-contaminated soil. Antonie van Leeuwenhoek 110, 803–809 (2017). https://doi.org/10.1007/s10482-017-0853-3

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    • DOI: https://doi.org/10.1007/s10482-017-0853-3

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