Abstract
Some land-use systems in Saskatchewan, Canada include the nitrogen-fixing trees buffaloberry (Shepherdia argentea Nutt.), caragana (Caragana arborescens Lam.) and sea buckthorn (Hippophae rhamnoides L.). These species provide various ecological functions such as ameliorating soil moisture, light and temperature but little work has been done quantifying biological nitrogen fixation by these species. Greenhouse experiments were conducted to quantify N2-fixation using the 15N natural abundance and the 15N dilution methods. Buffaloberry failed to form nodules in all but one of the four replicates in the natural abundance experiment. Using the 15N dilution method, the percentage of N derived from atmosphere (%Ndfa) in the shoot of buffaloberry averaged 64 %. For caragana, the mean %Ndfa was 59 and 65 % and seabuckthorn was 70 and 73 % measured using the natural abundance and dilution methods, respectively. Because of large variability in biomass production between plants grown in the natural abundance experiment and the dilution experiment, the amounts of N2 fixed also were very variable. Buffaloberry fixed an average of 0.89 g N m−2; the average for caragana ranged from 1.14 to 4.12 g N m−2 and seabuckthorn ranged from 0.85 to 3.77 g N m−2 in the natural abundance and dilution experiments, respectively. This corresponds to 16 kg N ha−1 year−1 for buffaloberry; an average of 15–73 kg N ha−1 year−1 in caragana and 11–67 kg N ha−1 year−1 in seabuckthorn. The substantial amounts of N2 fixed by these species indicate that they have the potential to contribute to the overall N balance in land-use systems in which they are included.
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References
Binkley D (1997) Bioassays of the influence of Eucalyptus saligna and Albizia falcataria on soil nutrient supply and limitation. For Ecol Manage 91:229–234
Boddey RM, Peoples MB, Palmer B, Dart PJ (2000) Use of the 15N natural abundance technique to quantify biological nitrogen fixation by woody perennials. Nutr Cycl Agroecosyst 57:235–270
Busse MD (2000) Suitability and use of 15N-isotope dilution method to estimate nitrogen fixation by actinorhizal shrubs. For Ecol Manag 136:85–95
Busse MD, Jurgensen MF, Page-Dumroese DS, Powers RF (2007) Contribution of actinorhizal shrubs to site fertility in a Northern California mixed pine forest. For Ecol Manag 244:68–75
Eriksen J, Høgh-Jensen H (1998) Variations in the natural abundance of 15N in ryegrass/white clover shoot material as influenced by cattle grazing. Plant Soil 205:67–76
Gathumbi SM, Cadisch G, Giller KE (2002) 15N natural abundance as a tool for assessing N2-fixation of herbaceous, shrub and tree legumes in improved fallows. Soil Biol Biochem 34:1059–1071
Gentili F, Huss-Danell K (2002) Phosphorus modifies the effects of nitrogen on nodulation in split-root systems of Hippophae rhamnoides. New Phytol 153:53–61
Gulden RH, Vessey JK (1998) Low concentrations of ammonium inhibit specific nodulation (nodule number g−1 root DW) in soybean (Glycine max [L.] Merr.). Plant Soil 198:127–136
Hauggaard-Nielsen H, Holdensen L, Wulfsohn D, Jensen ES (2010) Spatial variation of N2-fixation in field pea (Pisum sativum L.) at the field scale determined by the 15N natural abundance method. Plant Soil 327:167–184
Hendershot WH, Lalande H, Duquette M (2008) Soil reaction and exchangeable acidity. In: Carter MR, Gregorich EG (eds) Soil sampling and methods of analysis. CRC Press, Boca Raton, pp 173–214
Hendrickson OQ, Burgess D (1989) Nitrogen-fixing plants in a cut-over lodgepole pine stand of southern British Columbia. Can J For Res 19:936–939
Herridge DF, Peoples MB, Boddey RM (2008) Global inputs of biological nitrogen fixation in agricultural systems. Plant Soil 311:1–18
Hu ZY, Zhao FJ, McGrath SP (2005) Sulphur fractionation in calcareous soils and bioavailability to plants. Plant Soil 268:103–109
Isaac ME, Hinsinger P, Harmand JM (2012) Nitrogen and phosphorus economy of a legume tree-cereal intercropping system under controlled conditions. Sci Total Environ 434:71–78
Jalonen R, Nygren P, Sierra J (2009) Root exudates of a legume tree as a nitrogen source for a tropical fodder grass. Nutr Cycl Agroecosyst 85:203–213
Jose S, Gordon AM (2008) Applying ecological knowledge to agroforestry design: a synthesis. In: Jose S, Gordon AM (eds) Toward agroforestry design: an ecological approach. Springer, New York, pp 3–17
Jose S, Gillespie AR, Pallardy SG (2004) Interspecific interactions in temperate agroforestry. Agrofor Syst 61:237–255
Khanna PK (1998) Nutrient cycling under mixed-species tree systems in Southeast Asia. Agrofor Syst 38:99–120
Ledgard SF (1989) Nutrition, moisture and rhizobial strain influence isotopic fractionation during N2 fixation in pasture legumes. Soil Biol Biochem 21:65–68
Mafongoya PL, Giller KE, Odee D, Gathumbi S, Ndufa SK, Sitompul SM (2004) Benefiting from N2-fixation and managing rhizobia. In: van Noordwijk M et al (eds) Belowground interactions in tropical agroecosystems: concepts and models with multiple plant components. CABI International, Wallingford, pp 227–242
Maynard DG, Kalra YP, Crumbaugh JA (2008) Nitrate and exchangeable ammonium nitrogen. In: Carter MR, Gregorich EG (eds) Soil sampling and methods of analysis. CRC Press, Boca Raton, pp 71–80
Miller ZM (2011) An investigation of nitrogen fixation by russet buffaloberry in Colorado conifer forests. MSc thesis, Colorado State University, Fort Collins, Colorado
Moukoumi J, Farrell RE, Van Rees KJC, Hynes RK, Bélanger N (2012) Growth and nitrogen dynamics of juvenile short rotation intensive cultures of pure and mixed Salix miyabeana and Caragana arborescens. Bio Res 5:719–732
Murray U, Herridge D, Peoples M, Cadisch G, B. Boddey, Giller K, Alves B, Chalk P (2008) Measuring plant-associated nitrogen fixation in agricultural systems. ACIAR Monograph No. 132:131–162
Parrota JA, Baker DD, Fried M (1994) Application of 15N enrichment methodologies to estimate nitrogen fixation in Casuarina equisetifolia. Can J For Res 24:201–207
Peoples MB, Herridge DF (1990) Nitrogen fixation by legumes in tropical and subtropical agriculture. Advan Agron 44:155–223
Peoples MB, Brockwell J, Herridge DF, Rochester IJ, Alves BJR, Urquiaga S (2009) The contributions of nitrogen-fixing crop legumes to the productivity of agricultural systems. Symbiosis 48:1–17
Pollock T, Svendsen E (2008) Trees and shrubs for agroforestry on the prairies: adapted species available through the Prairie Shelterbelt Program. Agriculture and Agri-Food Canada, Indian Head, SK, pp 31
Qian P, Schoenau JJ, Karamanos RE (1994) Simultaneous extraction of available phosphorus and potassium with a new soil test: a modification of Kelowna extraction. Commun Soil Sci Plant Anal 25:627–635
Ray JD, Heatherly LG, Fritschi FB (2006) Influence of large amounts of nitrogen applied at planting on non-irrigated and irrigated soybean. Crop Sci 46:52–60
Rennie RJ, Dubetz S (1986) Nitrogen-15-determined nitrogen fixation in field-grown chickpea, lentil, faba bean, and field pea. Agron J 78:654–660
Robinson D, Handley LL, Scrimgeour CM, Gordon DC, Forster BP, Ellis RP (2000) Using stable isotope natural abundance (δ15N and δ 13C) to integrate the stress response of wild barley (Hordeum spontaneum C. Kock.) genotypes. J Exp Bot 51:41–50
SAS Institute. 2008. SAS/STAT user’s guide, vers. 9.2. SAS Institute Inc., Cary, NC
Saxton AM (1998) A macro for converting mean separation output to letter groupings in Proc Mixed. In: 23rd SAS Users Group Intl., SAS Institute Inc., Cary, NC, USA, pp 1243–1246
Tahir MM, Abbasi MK, Rahim N, Khaliq A, Kazmi MH (2009) Effect of Rhizobium inoculation and NP fertilization on growth, yield and nodulation of soybean (Glycine max L.) in the sub-humid hilly region of Rawalakot Azad Jammu and Kashmir, Pakistan. Afr J Biotechnol 8:6191–6200
Thevathasan NV, Gordon AM, Bradley R, Cogliastro A, Folkard P, Grant R, Kort J, Liggins L, Njenga F, Olivier A, Pharo C, Powell G, Rivest D, Schiks T, Trotter D, van Rees K, Whalen J, Zabek L (2012) Agroforestry research and development in Canada: the way forward. Advan Agrofor 9:247–283
Unkovich MJ, Pate JS, Sanford P, Armstrong EL (1994) Potential precision of the delta 15N natural abundance method in field estimates of nitrogen fixation by crop and pasture legumes in south-west Australia. Aust J Agric Res 45:119–132
Voisin AS, Salon C, Nunier-Jolain NG, Ney B (2002) Quantitative effects of soil nitrate, growth potential and phenology on symbiotic nitrogen fixation of pea (Pisum sativum L.). Plant Soil 243:31–42
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Issah, G., Kimaro, A.A., Kort, J. et al. Quantifying biological nitrogen fixation of agroforestry shrub species using 15N dilution techniques under greenhouse conditions. Agroforest Syst 88, 607–617 (2014). https://doi.org/10.1007/s10457-014-9706-5
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DOI: https://doi.org/10.1007/s10457-014-9706-5