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Leveraging Superficially Porous Particle Technology to Develop High-Throughput HPLC Methods for Small-Molecule Drug Discovery

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Abstract

Superficially porous particle (SPP) columns have gained considerable momentum over fully porous particle (FPP) columns among many chromatographers. However, despite several advantages, the SPP columns are not always been used effectively with traditional HPLC. The present study explores the possibility of reducing the runtime of HPLC methods using SPP column technology without compromising the efficiency of high-throughput analysis using conventional HPLC systems. An optimized method was developed by exploiting the advantages of SPP column technology and its effectiveness demonstrated in two separate SPP columns, Kinetex EVO C18 and Accucore C18. By altering method parameters such as flow rate, gradient time, and photodiode array (PDA) detector acquisition rate, we showed that an optimized method is more efficient than the regular method. The experimental results showed significant improvement in peak capacity, peak symmetry, theoretical plate numbers, and peak resolution for the SPP column within a short runtime of 7 min. We demonstrated that the present optimized method helped to reduce runtime by 50% and provide ~ 31% net organic solvent savings, exhibiting a greener approach to methods and method development.

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Data availability

Authors declare that the data supporting the findings of this study are available within the paper and its Supplementary Information files. If any raw data files be needed in another format, they are available from the corresponding author upon reasonable request. Supplementary files can be accessed via the following link: https://doi.org/10.1007/s10337-023-04259-y

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Acknowledgements

The authors would like to thank Bristol-Myers Squibb & Syngene International Ltd for providing infrastructure support to do this work.

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Discovery Analytical Sciences, Biocon Bristol-Myers Squibb R&D Center (BBRC), Syngene International Ltd, Biocon Park, Plot No. 2 & 3, Bommasandra IV Phase, Jigani Link Road, Bangalore, 560099, India

    Nancharaiah Tellakula, Mallikarjuna Guna, Mitalee Das, Vaishali Bane, Siddheshwar Kisan Chauthe, Muralidhararao Bagadi, Khemraj Bairwa & Amrita Roy

  2. Small Molecule Drug Discovery, Bristol-Myers Squibb Research and Development, P.O. Box 5400, Princeton, NJ, 08543-4000, USA

    Arvind Mathur

Authors
  1. Nancharaiah Tellakula
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  2. Mallikarjuna Guna
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  3. Mitalee Das
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Contributions

NT and MG: Data curation, visualization and investigation. MD, VB and KB: Writing- Data investigation, validation, and manuscript drafting. SKC: Data curation. MB and AR: Conceptualization, methodology, validation, manuscript. AM: Supervision, reviewing and funding acquisition.

Corresponding authors

Correspondence to Khemraj Bairwa or Amrita Roy.

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The authors of this article declare no competing financial, professional and personal interest that might have influenced the work described in this manuscript.

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Tellakula, N., Guna, M., Das, M. et al. Leveraging Superficially Porous Particle Technology to Develop High-Throughput HPLC Methods for Small-Molecule Drug Discovery. Chromatographia 86, 437–446 (2023). https://doi.org/10.1007/s10337-023-04259-y

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