Skip to main content
Log in

Graphene Oxide/Plane Cocoon Double Membrane Solid Phase Microextraction for the Concentration of Alkaloids in Coptis chinensis

  • Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A new graphene oxide/plane cocoon double membrane solid phase microextraction, combined with HPLC, was proposed and employed for concentration and determination of the trace levels of four alkaloids (jatrorrhizine, coptisine, palmatine and berberine) in Coptis chinensis. In this method, a special double solid membrane was prepared to extract four alkaloids. After extraction, the enriched target analytes on the membrane were dissociated by methanol and analyzed by HPLC. The main variables affecting the method were investigated and optimized. Under the optimized conditions, linear range of coptisine was 0.005–1.5 µg mL−1, the other three were 0.001–1.5 µg mL−1; limit of detection and limit of quantification of jatrorrhizine, coptisine, palmatine, berberine were 0.3, 1.0, 0.1, 0.2 ng mL−1and 0.8, 1.5, 0.8, 0.7 ng mL−1, respectively; EFs of four alkaloids were 108, 85, 112 and 122, respectively. Furthermore, the relative standard deviations of intra-day and the inter-day were 1.2–4.6% and 5.7–10.4%, respectively. The average recoveries were 95.1–105.0%. Also, the microextraction mechanism was described. The results demonstrated that this approach is a simple and reliable, and can be used to help establish sensitive quality control methods of the active compounds in C. chinensis.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Arthur CL, Pawliszyn J (1990) Solid phase microextraction with thermal desorption using fused silica optical fibers. Anal Chem 62:2145–2148

    Article  CAS  Google Scholar 

  2. Chai XL, Jia JP, Sun TH, Wang YL (2008) Suitability of a novel circulating cooling SPME for analysis of organophosphorous pesticides in tomatoes. Chromatographia 67:309–313

    Article  CAS  Google Scholar 

  3. Yang L, Said R, Abdel-Rehim M (2017) Sorbent, device, matrix and application in microextraction by packed sorbent (MEPS): a review. J Chromatogr B Analyt Technol Biomed Life Sci 1043:33–43

    Article  CAS  Google Scholar 

  4. Zhang PZ, Jin LX, Chen YL (2006) The production technology of plane cocoon. Bull Seric 37:60

    Google Scholar 

  5. Zhang YQ (2001) Applications of natural silk protein sericin in biomaterials. Biotechnol Adv 20:91–100

    Article  CAS  Google Scholar 

  6. Kudin KN, Ozbas B, Schniepp HC, Prud’homme RK, Aksay IA, Car R (2008) Raman spectra of graphite oxide and functionalized graphene sheets. Nano Lett 8:36–41

    Article  CAS  Google Scholar 

  7. Allen MJ, Tung VC, Kaner RB (2010) Honeycomb carbon a review of graphene. Chem Rev 110:132–145

    Article  CAS  Google Scholar 

  8. Zhu XY, Zhu HT, Chen CY, Qiu YH (2010) Research progress of stimulation effect of silk fibroin on immune cells. Int J Biomed Eng 33:231–234

    Google Scholar 

  9. Ma SB (2013) New pharmacological action and clinical action of Coptis chinensis China’s. Naturopathy 21:58

    Google Scholar 

  10. Zhang W, Chen Z (2013) Mussel inspired polydopamine functionalized poly (ether ether ketone) tube for online solid-phase microextraction high performance liquid chromatography and its application in analysis of protoberberine alkaloids in rat plasma. J Chromatogr A 1278:29–36

    Article  CAS  Google Scholar 

  11. Chen J, Wang F, Liu J, Lee FS, Wang X, Yang H (2008) Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Anal Chim Acta 613:184–195

    Article  CAS  Google Scholar 

  12. Meng J, Zhang W, Bao T, Chen Z (2015) Novel molecularly imprinted magnetic nanoparticles for the selective extraction of protoberberine alkaloids in herbs and rat plasma. J Sep Sci 38:2117–2125

    Article  CAS  Google Scholar 

  13. Wang J, Chen X, Bai XH, Ma WY, Zhang LS (2012) Hollow-fiber liquid phase microextraction and application in determining pKa of alkaloids. Chin Remedies Clin 12:277–280

    Google Scholar 

  14. Liu X, Xing RR, Chen X, Hu S, Sheng X, Bai XH (2014) Determination of protoberberine alkaloids in Coptis chinensis by microextraction and high performance liquid chromatography. Anal Lett 47:2655–2664

    Article  CAS  Google Scholar 

  15. Chen X, Wang J, Hu S, Bai XH (2016) Hollow-fiber double-solvent synergistic microextraction with high-performance liquid chromatography for the determination of antitumor alkaloids in Coptis chinensis. J Sep Sci 39:827–834

    Article  CAS  Google Scholar 

  16. Gu H, Zhou NL, Fan YT, Wang XD, Li WX, Shen J (2013) Anticoagulant properties on carboxylated graphene oxide-glutamic acid composite. Chem J Chin Univ 34:479–484

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Xuan Chen.

Ethics declarations

Funding

This study was funded by the National Nature Science Foundation of China (No. 81302734) and sponsored by the Fund for Shanxi Key Subjects construction (2016).

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 183 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, J., Chen, X., Hu, S. et al. Graphene Oxide/Plane Cocoon Double Membrane Solid Phase Microextraction for the Concentration of Alkaloids in Coptis chinensis . Chromatographia 80, 1467–1473 (2017). https://doi.org/10.1007/s10337-017-3357-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-017-3357-4

Keywords

Navigation