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Production of optically pure l(+)-lactic acid from waste plywood chips using an isolated thermotolerant Enterococcus faecalis SI at a pilot scale

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Utilization of renewable and low-cost lignocellulosic wastes has received major focus in industrial lactic acid production. The use of high solid loadings in biomass pretreatment potentially offers advantages over low solid loadings including higher lactic acid concentration with decreased production and capital costs. In this study, an isolated Enterococcus faecalis SI with optimal temperature 42 °C was used to produce optically pure l-lactic acid (> 99%) from enzyme-saccharified hydrolysates of acid-impregnated steam explosion (AISE)-treated plywood chips. The l-lactic acid production increased by 10% at 5 L scale compared to the similar fermentation scheme reported by Wee et al. The fermentation with a high solid loading of 20% and 35% (w/v) AISE-pretreated plywood chips had been successfully scaled up to process development unit scale (100 L) and pilot scale (9 m3), respectively. This is the first report of pilot-scale lignocellulosic lactic acid fermentation by E. faecalis with high lactic acid titer (nearly 92 g L−1) and yield (0.97 kg kg−1). Therefore, large-scale l-lactic acid production by E. faecalis SI shows the potential application for industries.

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Acknowledgements

This study was supported by the National Energy Program-Phase II (Industry promotion platform for cellulosic ethanol with development of value-added biorefinery technology) from the government of Taiwan. The authors thank Mrs. Chun-Mei Huang for her technical support with analyzing the samples. The authors thank Dr. Wen-Hua Chen and her team for their assistance with the pretreatment tests.

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Authors and Affiliations

  1. Chemistry Division, Institute of Nuclear Energy Research, Atomic Energy Committee, Executive Yuan, No. 1000 Wenhua Rd. Jiaan Village, Longtan District, Taoyüan, 32546, Taiwan, ROC

    Shuo-Fu Yuan, Teng-Chieh Hsu, Chun-An Wang, Ming-Feng Jang, Yang-Cheng Kuo, Gia-Luen Guo & Wen-Song Hwang

  2. Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX, USA

    Shuo-Fu Yuan & Hal S. Alper

  3. McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX, 78712, USA

    Hal S. Alper

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  1. Shuo-Fu Yuan
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Correspondence to Hal S. Alper or Gia-Luen Guo.

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Yuan, SF., Hsu, TC., Wang, CA. et al. Production of optically pure l(+)-lactic acid from waste plywood chips using an isolated thermotolerant Enterococcus faecalis SI at a pilot scale. J Ind Microbiol Biotechnol 45, 961–970 (2018). https://doi.org/10.1007/s10295-018-2078-5

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