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Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacillus licheniformis

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Increase of pectinase activity is especially important in fermentation industry. Understanding of the metabolic mechanisms can find metabolic modulation approach to promote high yield of pectinase. Higher activity of pectinase was detected in DY1 than DY2, two strains of Bacillus licheniformis. GC–MS-based metabolomics identified differential metabolome of DY2 compared with DY1, characterizing the increased TCA cycle and biosynthesis of fatty acids. Elevated activity of pyruvate dehydrogenase (PDH), α-ketoglutaric dehydrogenase (KGDH) and succinate dehydrogenase (SDH) showed global elevation of carbon metabolism, which is consistent with the result that lowers glucose in DY2 than DY1. Inhibitors malonate, furfural and triclosan, of PDH, SDH and biosynthesis of fatty acids, promoted pectinase activity, where triclosan increased pectinase activity by 179%. These results indicate that functional metabolomics is an effective approach to understand metabolic mechanisms of fermentation production and provides clues to develop new methods for changing bacterial physiology and production.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31701844), Foundation of Marine Bioenzyme Engineering Innovation Service Platform (2014FJPT02), Priming Scientific Research Foundation of Fuzhou University, China (XRC-1661), State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, the Key Laboratory of the Ministry of Agriculture for Integrated Control of Crop Pests.

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  1. Fujian Key Laboratory of Marine Enzyme Engineering, College of Biological Sciences and Technology, Fuzhou University, No. 2 Xue Yuan Road, Fuzhou, 350108, Fujian, China

    Yi Guan, Di Yin, Xi Du & Xiuyun Ye

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  1. Yi Guan
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Correspondence to Yi Guan or Xiuyun Ye.

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Guan, Y., Yin, D., Du, X. et al. Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacillus licheniformis. J Ind Microbiol Biotechnol 45, 951–960 (2018). https://doi.org/10.1007/s10295-018-2071-z

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