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Process challenges relating to hematopoietic stem cell cultivation in bioreactors

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  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Hematopoietic stem cells (HSCs) are extremely useful in treating a wide range of diseases and have a variety of useful research applications. However, the routinely generated low in vitro concentrations of HSCs from current bioreactor manufacturing systems has been a hindrance to the full-scale application of these essential cellular materials. This has made the search for novel bioreactor systems for high-concentration HSC production a major research endeavour. This review addresses process challenges in relation to bioreactor development and optimisation for high-density HSC production under effective monitoring of essential culture parameters, such as pH, dissolved oxygen and nutrient uptake. It discusses different process strategies and bioreactor configurations for HSCs production from a commercial viability perspective, and also discusses recent advances in the field.

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Authors and Affiliations

  1. Bio Engineering Laboratory, Department of Chemical Engineering, Monash University, Wellington Road, Clayton, VIC, 3800, Australia

    Marcin Kowalczyk, Kathryn Waldron, Penia Kresnowati & Michael K. Danquah

Authors
  1. Marcin Kowalczyk
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  2. Kathryn Waldron
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  3. Penia Kresnowati
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  4. Michael K. Danquah
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Correspondence to Michael K. Danquah.

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Kowalczyk, M., Waldron, K., Kresnowati, P. et al. Process challenges relating to hematopoietic stem cell cultivation in bioreactors. J Ind Microbiol Biotechnol 38, 761–767 (2011). https://doi.org/10.1007/s10295-011-0951-6

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  • DOI: https://doi.org/10.1007/s10295-011-0951-6

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