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Identification of Japanese Astraeus, based on morphological and phylogenetic analyses

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Mycoscience

Abstract

To clarify the diversity of Astraeus in Japan, 35 collections of Astraeus basidiomata from Japan and Thailand were examined for their morphological characteristics and the nucleotide sequences of the rDNA ITS region and compared with several worldwide Astraeus species. Japanese Astraeus specimens were separated into two groups based on basidiome size, shape of exoperidium, and ornamentation and size of basidiospores. The phylogenetic tree analyses supported the separation, and the morphological groups belonged to different clades. The Japanese Astraeus group 1, morphologically matched to Astraeus hygromerticus var. koreanus, showed the closest phylogenetic relationship with Astraeus hygrometricus from North American and Mediterranean regions, suggesting that the Astraeus group 1 can be indentified as A. hygrometricus var. koreanus. Another Astraeus group, group 2, morphologically matched to A. hygrometricus s.l., showed a distinct monophyletic clade that was separated from A. hygrometricus complexes, indicating an undescribed species.

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Acknowledgments

We thank Susumu Takamatsu, Graduate School of Bioresources, Mie University, Japan, for teaching and discussing phylogenetic analyses. We also acknowledge Hiromi Sasaki, Naoki Endo, Masaru Hiroi, Taiga Kasuya, Numyen Kamonkunanon, Siwat Kamonkunanon, and Pakron Ket-In for their assistance with mushroom collections. This study was supported, in part, by a Grant-in-Aid for Scientific Research (no. 19380085) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Interdisciplinary Graduate School of Science and Technology, Shinshu University, Minami-minowa, Nagano, 399-4598, Japan

    Wanwisa Fangfuk, Masaki Fukuda & Akiyoshi Yamada

  2. Department of Applied Biology, Faculty of Science and Technology, Uttaradit Rajabhat University, Uttaradit, 53000, Thailand

    Ratchanee Petchang

  3. Department of Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand

    Chaiwat To-anun

  4. Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, Nagano, 399-4598, Japan

    Masaki Fukuda & Akiyoshi Yamada

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  1. Wanwisa Fangfuk
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  2. Ratchanee Petchang
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  3. Chaiwat To-anun
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  4. Masaki Fukuda
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  5. Akiyoshi Yamada
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Correspondence to Akiyoshi Yamada.

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Fangfuk, W., Petchang, R., To-anun, C. et al. Identification of Japanese Astraeus, based on morphological and phylogenetic analyses. Mycoscience 51, 291–299 (2010). https://doi.org/10.1007/s10267-010-0039-6

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  • DOI: https://doi.org/10.1007/s10267-010-0039-6

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