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Sustainable fruit-body formation of edible mycorrhizal Tricholoma species for 3 years in open pot culture with pine seedling hosts

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Mycoscience

Abstract

Three edible mycorrhizal mushrooms, Tricholoma portentosum, T. saponaceum, and T. terreum, that had formed ectomycorrhizas with Pinus densiflora seedlings in vitro, were maintained in open pot culture for 3 years under laboratory conditions. Tricholoma portentosum and T. saponaceum produced fruit bodies several times. For T. terreum, which produced a single fruit body in the third year, this is the first report of mushroom production under controlled conditions. Morphological observation of fruit bodies indicated that they were mature, i.e., well-organized cap, stem, and gills, and basidiospores. These results suggest that cultivation of these three edible Tricholoma mushrooms is feasible.

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References

  • R Agerer (Eds) (1987–2002) Colour atlas of Ectomycorrhizae, 1st–12th del Einhorn-Verlag Schwäisch Gmüd

    Google Scholar 

  • D Arola (1987) Mushroom demystified, 2nd edn Ten Speed Press Berkeley

    Google Scholar 

  • MI Bidartondo TD Bruns (2002) ArticleTitleFine-level mycorrhizal specificity in the Monotropoideae (Ericaceae): specificity for fungal species groups Mol Ecol 11 557–569 Occurrence Handle11918790 Occurrence Handle10.1046/j.0962-1083.2001.01443.x Occurrence Handle1:CAS:528:DC%2BD38XislCgt7s%3D

    Article  PubMed  CAS  Google Scholar 

  • J Breitenbach F Kränzlin (1991) Fungi of Switzerland, vol 3. Boletes and Agarics, part 1 Mykologia Luzern Lucerne

    Google Scholar 

  • JWG Cairney SM Chambers (1999) Ectomycorrhizal fungi: key genera in profile Springer Berlin

    Google Scholar 

  • E Danell (2002) Current research on chantharelle cultivation in Sweden I Hall Y Wang E Danell A Zambonelii (Eds) Edible mycorrhizal mushrooms and their cultivation Crop & Food Research Christchurch 1–4

    Google Scholar 

  • E Danell F Camacho (1997) ArticleTitleSuccessful cultivation of the golden chantharelle Nature (Lond) 385 303 Occurrence Handle10.1038/385303a0 Occurrence Handle1:CAS:528:DyaK2sXotFansg%3D%3D

    Article  CAS  Google Scholar 

  • C Godbout JA Fortin (1990) ArticleTitleCultural control of basidiome formation in Laccaria bicolor with container-grown white pine seedlings Mycol Res 94 1051–1058 Occurrence Handle10.1016/S0953-7562(09)81332-4

    Article  Google Scholar 

  • C Godbout JA Fortin (1992) ArticleTitleEffects of nitrogen fertilization and photoperiod on basidiome formation of Laccaria bicolor associated with container-grown jack pine seedling Can J Bot 70 181–185

    Google Scholar 

  • A Guerin-Laguette C Plassard D Mousain (2000) ArticleTitleEffects of experimental conditions on mycorrhizal relationships between Pinus sylvestris and Lactarius deliciosus and unprecedented fruit-body formation of the Saffron milk cap under controlled soilless conditions Can J Microbiol 46 790–799 Occurrence Handle11006839 Occurrence Handle10.1139/cjm-46-9-790 Occurrence Handle1:CAS:528:DC%2BD3cXmvVGlu7o%3D

    Article  PubMed  CAS  Google Scholar 

  • A Guerin-Laguette K Shindo N Matsushita K Suzuki F Lapeyrie (2004) ArticleTitleThe mycorrhizal fungus Tricholoma matsutake stimulates Pinus densiflora seedling growth in vitro Mycorrhiza 14 397–400 Occurrence Handle15322965 Occurrence Handle10.1007/s00572-004-0322-5

    Article  PubMed  Google Scholar 

  • I Hall Y Wang E Danell A Zambonelii (Eds) (2002) Edible mycorrhizal mushrooms and their cultivation Crop & Food Research Christchurch

    Google Scholar 

  • D Hosford D Pilz R Molina M Amaranthus (1997) Ecology and management of the commercially harvested American matsutake mushrooms. PNW-GTR-412 USDA Forest Service Washington, DC

    Google Scholar 

  • R Imazeki T Hongo (1987) Colored illustrations of mushrooms of Japan, I (in Japanese) Hoikusha Osaka

    Google Scholar 

  • K Iwase (1997) ArticleTitleCultivation of mycorrhizal mushrooms Food Rev Int 13 431–442 Occurrence Handle10.1080/87559129709541130

    Article  Google Scholar 

  • M Kawai (1997) ArticleTitleArtificial ectomycorrhiza formation on roots of air-layered Pinus densiflora saplings by inoculation with Lyophyllum shimeji Mycologia 89 228–232 Occurrence Handle10.2307/3761075

    Article  Google Scholar 

  • K Kinugawa M Ogawa (2000) Mushroom guide book (in Japanese) Asakura Shoten Tokyo

    Google Scholar 

  • A Ohta (1994a) ArticleTitleSome cultural characteristics of mycelia of a mycorrhizal fungus, Lyophyllum shimeji Mycoscience 35 83–87 Occurrence Handle10.1007/BF02268533

    Article  Google Scholar 

  • A Ohta (1994b) ArticleTitleProduction of fruit-bodies of a mycorrhizal fungus, Lyophyllum shimeji, in pure culture Mycoscience 35 147–151 Occurrence Handle10.1007/BF02318492

    Article  Google Scholar 

  • A Riva (2003) Tricholoma (Fr.) Staude. Fungi Europaei 3 Edizioni Candusso Allassio

    Google Scholar 

  • SE Smith DJ Read (1997) Mycorrhizal symbiosis, 2nd edn Academic Press San Diego

    Google Scholar 

  • K Suzuki (2005) ArticleTitleEctomycorrhizal ecophysiology and the puzzle of Tricholoma matsutake (in Japanese) J Jpn For Soc 87 90–102 Occurrence Handle1:CAS:528:DC%2BD2MXjvVyrtbw%3D

    CAS  Google Scholar 

  • L-M Vaario A Guerin-Laguette WM Gill F Lapeyrie K Suzuki (2000) ArticleTitleOnly two weeks are required for Tricholoma matsutake to differentiate ectomycorrhizal Hartig net structures in roots of Pinus densiflora seedlings cultivated on artificial substrate J For Res 5 293–297

    Google Scholar 

  • Y Wang IR Hall LA Evans (1997) ArticleTitleEctomycorrhizal fungi with edible fruiting bodies. 1. Tricholoma matsutake and related fungi Econ Bot 51 311–327

    Google Scholar 

  • A Yamada S Kanekawa M Ohmasa (1999a) ArticleTitleEctomycorrhiza formation of Tricholoma matsutake on Pinus densiflora Mycoscience 40 193–198 Occurrence Handle10.1007/BF02464298

    Article  Google Scholar 

  • A Yamada K Maeda M Ohmasa (1999b) ArticleTitleEctomycorrhizal formation of Tricholoma matsutake isolates on seedlings of Pinus densiflora in vitro Mycoscience 40 455–463 Occurrence Handle10.1007/BF02461022

    Article  Google Scholar 

  • A Yamada T Ogura M Ohmasa (2001a) ArticleTitleCultivation of mushrooms of edible ectomycorrhizal fungi associated with Pinus densiflora by in vitro mycorrhizal synthesis. I. Primordium and basidiocarp formation in open-pot culture Mycorrhiza 11 59–66 Occurrence Handle10.1007/s005720000092

    Article  Google Scholar 

  • A Yamada T Ogura M Ohmasa (2001b) ArticleTitleCultivation of mushrooms of edible ectomycorrhizal fungi associated with Pinus densiflora by in vitro mycorrhizal synthesis. II. Morphology of mycorrhizas in open-pot soil Mycorrhiza 11 67–81 Occurrence Handle10.1007/s005720000093 Occurrence Handle1:CAS:528:DC%2BD3MXkt12gs78%3D

    Article  CAS  Google Scholar 

  • A Yamada H Kobayashi H Murata (2003) ArticleTitleTricholoma matsutake IFO6933 and IFO30604, “matsutake” isolates that have been maintained on slants and widely used in vitro for a quarter to half a century, can form ectomycorrhiza in Pinus densiflora Mycoscience 44 249–251

    Google Scholar 

  • A Yamada K Maeda H Kobayashi H Murata (2006) ArticleTitleEctomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring “shiro Mycorrhiza 16 111–116 Occurrence Handle16273392 Occurrence Handle10.1007/s00572-005-0021-x

    Article  PubMed  Google Scholar 

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Correspondence to Akiyoshi Yamada.

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Yamada, A., Kobayashi, H., Ogura, T. et al. Sustainable fruit-body formation of edible mycorrhizal Tricholoma species for 3 years in open pot culture with pine seedling hosts. Mycoscience 48, 104–108 (2007). https://doi.org/10.1007/s10267-006-0338-0

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