Abstract
Three cryptic species of Ceratopteris thalictroides, named the south type, the north type and the third type, were examined for their morphological characteristics, using sporophytes cultivated under common conditions. The discriminant analysis for leaf characters followed by one-way layout ANOVA or Kruskal–Wallis test for selected combinations of characters revealed that the following characters may be effective for identifying the three types: the relative lengths of stipe to blade and to pinna, the degree of dissection, the segment densities on rachis and pinna rachis, and the elongation degree of ultimate segments. The number of annulus cells on sporangia also proved to be a possible distinguishing character. As morphological data were obtained from a limited number of cultivated sporophytes, they are regarded as not definitive, but only referential diagnostic characters of the types and should be utilized not solely, but collectively, to avoid identification errors of the types. An identification trial using herbarium specimens proved these diagnostic characters to be useful to a considerable degree.
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Acknowledgments
I am much indebted to Dr. Bayu Adjie of Chiba University for the chloroplast DNA analysis of the materials IN5 and IN6. I am also grateful to the curator of the herbarium of Kyoto University for permission to utilize fertile leaf segments of Ceratopteris specimens for this study.
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10265_2008_159_MOESM2_ESM.bmp
S2. Plots of individuals on the axes of the first two canonical discriminant functions. A, sterile leaf; B, fertile leaf. S, N and T, the groups S, N and T; x, group centroid. (BMP 1506 kb)
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Masuyama, S. Cryptic species in the fern Ceratopteris thalictroides (Parkeriaceae). III. Referential diagnostic characters of three cryptic species. J Plant Res 121, 279–286 (2008). https://doi.org/10.1007/s10265-008-0159-7
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DOI: https://doi.org/10.1007/s10265-008-0159-7