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Expression of tetraspanins NET-6 and CD151 in breast cancer as a potential tumor biomarker

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Abstract

Tetraspanins have been implicated in multiple biological functions including protein networking and cell signaling. NET-6 (TSPAN 13) has been demonstrated to be a tumor suppressor gene in breast cancer, while CD151 is more likely to act as an oncogene. However, the biological function of both proteins is still inconclusive. Immunohistochemistry was used to analyze the expression of NET-6 and CD151 proteins in breast tumors and benign epithelial cells. The cellular expression of both markers was correlated with HER2, ER, and PR status as well as tumor grade, Ki-67 scores, invasion, and metastasis. Expression of NET-6 and CD151 was variable both in tumors and in benign epithelial cells. Expression of NET-6 and CD151 was stronger in tumors than in benign epithelial cells. The expression of NET-6 was also stronger in HER2-negative, low-grade, lymphovascular invasion-negative, and non-metastatic breast tumors. There was no correlation between NET-6 expression and ER, or PR, or triple-negative status. There was no correlation between CD151 expression and HER2, ER, PR, or triple-negative status, tumor grade, or Ki-67 scores, invasion, and metastasis. The expression of tetraspanins NET-6 and CD151 may indicate an alteration of their biological function during neoplastic transformation. NET-6 expression in tumors might be a potential marker indicating the outcome of breast cancer.

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Acknowledgements

This study was funded by grants from the Guangxi Bureau of Science and Technology, No. 1598013-3 (PI: Huayi Huang) and No. 2017GXNSFAA198043 (PI: Huayi Huang) and a grant from The Health and Family Planning Commission of Guangxi Zhuang Autonomous Region, No. Z2015353 (PI: Huayi Huang).

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Author notes

  1. Liejun Jiang, Xiliu Zhang, and Huayi Huang have contributed equally to this work.

Authors and Affiliations

  1. Department of Laboratory Medicine, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guangxi, China

    Liejun Jiang & Huayi Huang

  2. Department of Pathology, The First Affiliated Hospital of Guangxi Traditional Chinese Medicine, Nanning, 530023, Guangxi, China

    Xiliu Zhang

  3. Department of Population Sciences, City of Hope National Medical Center, Duarte, 91010, CA, USA

    Joseph Geradts

  4. Department of Pathology, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guangxi, China

    Qiang Wei

  5. Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA

    Steven Hochwald & Huayi Huang

  6. Department of Pathology, Huayin Medical Laboratory, Guangzhou, 510670, Guangdong, China

    Hui Xu

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  1. Liejun Jiang
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Correspondence to Huayi Huang.

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Ethical approval

The study was approved by the Institute Review Boards of Guangzhou Huayin Medical Laboratory and the People’s Hospital of Guangxi Zhuang Autonomous Region for the use of human materials.

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This study was waived for the patients’ informed consent from the above-mentioned institutions because the study was a retrospective with pathology archived tissues.

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Jiang, L., Zhang, X., Geradts, J. et al. Expression of tetraspanins NET-6 and CD151 in breast cancer as a potential tumor biomarker. Clin Exp Med 19, 377–384 (2019). https://doi.org/10.1007/s10238-019-00554-x

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  • DOI: https://doi.org/10.1007/s10238-019-00554-x

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