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Long noncoding RNA expression profiles in chondrogenic and hypertrophic differentiation of mouse mesenchymal stem cells

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Abstract

Long noncoding RNAs (lncRNAs) are important regulators for a variety of biological processes. Chondrogenic differentiation of mesenchymal stem cells (MSCs) is a crucial stage in chondrogenesis while chondrocyte hypertrophy is related to endochondral ossification and osteoarthritis. However, the effects of lncRNAs on chondrogenic and hypertrophic differentiation of mouse MSCs are unclear. To explore the potential mechanisms of lncRNAs during chondrogenesis and chondrocyte hypertrophy, microarray was performed to investigate the expression profiles of lncRNA and mRNA in MSCs, pre-chondrocytes, and hypertrophic chondrocytes. Then, we validated microarray data by RT-PCR and screened three lncRNAs from upregulating groups during chondrogenesis and chondrocyte hypertrophy respectively. After downregulating any of the above lncRNAs, we found that the expression of chondrogenesis-related genes such as Sox9 and Col2a1 and hypertrophy-related genes including Runx2 and Col10a1 was inhibited, respectively. Furthermore, the target genes of above lncRNAs were predicted by bioinformatics approaches. Gene ontology and Kyoto encyclopedia of genes and genome biological pathway analysis were also made to speculate the functions of above lncRNAs. In conclusion, the study first revealed the expression profile of lncRNAs in chondrogenic and hypertrophic differentiations of mouse MSCs and presented a new prospect for the underlying mechanisms of chondrogenesis and endochondral ossification.

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Abbreviations

lncRNAs:

Long noncoding RNAs

MSCs:

Mesenchymal stem cells

GO:

Gene ontology

Mmp13:

Matrix metalloproteinase 13

KEGG:

Kyoto encyclopedia of genes and genomes

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Acknowledgments

This work was supported by grants from the Nature Science Foundation of China (81572164) and the National Key Technology Research and Development Program of China (2017YFC1103300).

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Authors and Affiliations

  1. Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Gaotanyan Street No. 30, Chongqing, 400038, China

    Zhen Cao, Jianmei Li, Yun Bai, Ce Dou, Chuan Liu, Fei Kang, Xiaoshan Gong, Haibin Ding & Shiwu Dong

  2. Department of Anatomy, Third Military Medical University, Chongqing, 400038, China

    Zhen Cao

  3. School of Pathology and Laboratory Medicine, The University of Western Australia, Nedlands, WA, 6009, Australia

    Song Huang

  4. National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

    Ce Dou & Tianyong Hou

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  1. Zhen Cao
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  2. Song Huang
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Correspondence to Shiwu Dong.

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Cao, Z., Huang, S., Li, J. et al. Long noncoding RNA expression profiles in chondrogenic and hypertrophic differentiation of mouse mesenchymal stem cells. Funct Integr Genomics 17, 739–749 (2017). https://doi.org/10.1007/s10142-017-0569-5

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  • DOI: https://doi.org/10.1007/s10142-017-0569-5

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