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Administration of Probiotics Improves the Brine Shrimp Production and Prevents Detrimental Effects of Pathogenic Vibrio Species

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Abstract

In this study, we evaluated a consortium of probiotic bacteria as an environmentally-friendly strategy for controlling pathogenic Vibrio species during the brine shrimp incubation period. Probiotic strains were initially selected on basis of (i) their ability to colonize the cyst surfaces, (ii) their absence of cross-inhibitory effects, and (iii) no detrimental effect on cyst hatching. The cysts and nauplius surfaces were immediately colonized after the application of selected probiotic strains, without detrimental effects on survival. Ten probiotic strains were mixed at similar proportions (probiotic consortium) and evaluated at different concentrations into brine shrimp cultures during incubation and early stages of development. Subsequently, these cultures were challenged with Vibrio parahaemolyticus and Vibrio harveyi. The probiotic consortium was effective to reduce the abundance of pathogenic Vibrio species and to prevent the mortality during Vibrio challenges; however, its effect was concentration-dependent and was successful at a starting concentration of 1.8 × 106 CFU/ml. Our results suggest that this probiotic consortium offers an alternative to antimicrobial agents routinely used to reduce the incidence and prevalence of pathogenic Vibrio species in brine shrimp production.

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Acknowledgments

This research was supported by projects SIP 20100865 and CONACyT 085033. EQG thanks CONACyT for the support through grant number, 34984, and the Secretariat for Research and Graduate studies (SIP-IPN) for the support through PIFI grant.

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Authors and Affiliations

  1. Cátedras-CIBNOR, Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), 23096, La Paz, B.C.S., Mexico

    Eduardo Quiroz-Guzmán

  2. Centro de Investigaciones Biológicas del Noroeste, S.C.(CIBNOR), 23096, La Paz, B.C.S., Mexico

    Ricardo Vázquez-Juárez

  3. Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Guasave, Sinaloa, Mexico

    Antonio Luna-González

  4. Catalan Institute for Water Research (ICRA), 17003, Girona, Spain

    José L. Balcázar

  5. Microbiology and Molecular Biology Lab, Instituto Politécnico Nacional-CICIMAR, Av. Instituto Politécnico Nacional s/n, Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., Mexico

    Diana R. Barajas-Sandoval & Sergio F. Martínez-Díaz

Authors
  1. Eduardo Quiroz-Guzmán
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  2. Ricardo Vázquez-Juárez
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  3. Antonio Luna-González
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  4. José L. Balcázar
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  5. Diana R. Barajas-Sandoval
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  6. Sergio F. Martínez-Díaz
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Corresponding author

Correspondence to Sergio F. Martínez-Díaz.

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Quiroz-Guzmán, E., Vázquez-Juárez, R., Luna-González, A. et al. Administration of Probiotics Improves the Brine Shrimp Production and Prevents Detrimental Effects of Pathogenic Vibrio Species. Mar Biotechnol 20, 512–519 (2018). https://doi.org/10.1007/s10126-018-9822-8

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  • DOI: https://doi.org/10.1007/s10126-018-9822-8

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