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Photobiomodulation by dual-wavelength low-power laser effects on infected pressure ulcers

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A Correction to this article was published on 18 November 2019

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Abstract

The aim of this study was to evaluate the effects of photobiomodulation (PBM) by dual-wavelength low-power lasers on the healing and bacterial bioburden of pressure ulcer (PU) models. Twenty-five male Swiss mice were divided into five equal groups. Ischemia reperfusion cycles were employed to cause PU formation by the external application of magnetic plates. Immediately after wounding, a suspension of Pantoea agglomerans was applied at the base of all the wounds of the infected groups, using a calibrated pipette. PBM (simultaneous emission at 660 and 808 nm, 142.8 J/cm2, in continuous wave emission mode) was applied to the PUs for 14 sessions. The animals were euthanized 14 days after PU induction, and their tissues were analyzed for wound contraction and reepithelialization, epidermis thickness, bacterial survival, and IL-1β and IL-10 mRNA level evaluations. The PU areas appeared larger in the mice from the infected groups than in those in the laser group 4 days after PU induction and presented incomplete reepithelialization 14 days after PU induction. However, the PBM accelerated the wound healing in the infected + laser group compared with the infected group 11 and 14 days following the PU induction. The infected and irradiated PUs exhibited a thinner neo-epidermis than those in the infected group, and the bacterial survival decreased in the laser group; the relative expression IL-1β mRNA levels demonstrated an increasing tendency while the relative expression IL-10 mRNA levels demonstrated a decreasing tendency in the infected + laser and laser groups. These results suggest that PBM improves healing by killing or inhibiting bacteria in PUs as well as by accelerating the wound healing, resulting in tissue repair.

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Change history

  • 18 November 2019

    The author name Maria Maria Côrtes Thomé Lima was incorrectly captured in the original article. The correct author name should be Andrezza Maria Côrtes Thomé Lima. The original article has been corrected.

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Funding

This study was funded by Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Universidade do Estado do Rio de Janeiro (UERJ), and Universidade Severino Sombra (USS).

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Authors and Affiliations

  1. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Boulevard Vinte e Oito de Setembro, Avenida 28 de Setembro, 87, Vila Isabel, Rio de Janeiro, 20551030, Brazil

    Andrezza Maria Côrtes Thomé Lima, Luiz Philippe da Silva Sergio, André Luiz Mencalha & Adenilson de Souza da Fonseca

  2. Pró Reitoria de Pesquisa e Pós Graduação, Universidade de Vassouras, Av. Expedicionário Oswaldo de Almeida Ramos, 280, Centro, Vassouras, 277000000, Brazil

    Larissa Alexsandra da Silva Neto Trajano, Bianca Paschoal de Souza, João Pedro da Motta Mendes, Adriano Fonseca Ribeiro Cardoso, Caroline Pisa Figueira, Beatriz dos Anjos Tavares, Daniella Sousa Figueira & Eduardo Tavares Lima Trajano

  3. Centro de Ciências da Saúde, Centro Universitário Serra dos Órgãos, Avenida Alberto Torres, 111, Teresópolis, Rio de Janeiro, 25964004, Brazil

    Adenilson de Souza da Fonseca

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  1. Andrezza Maria Côrtes Thomé Lima
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  2. Luiz Philippe da Silva Sergio
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Correspondence to Andrezza Maria Côrtes Thomé Lima.

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Ethical approval

All procedures were carried out in accordance with the Brazilian Legislation (no. 11.794, from October 8th, 2008) and were approved by the Ethical Committee for Animal Use of Universidade Severino Sombra (CEUA/007/2015).

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The authors declare that they have no conflict of interest.

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The original version of this article was revised: The author name Maria Maria Côrtes Thomé Lima was incorrectly captured in the original article. The correct author name should be Andrezza Maria Côrtes Thomé Lima.

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Thomé Lima, A.M.C., da Silva Sergio, L.P., da Silva Neto Trajano, L.A. et al. Photobiomodulation by dual-wavelength low-power laser effects on infected pressure ulcers. Lasers Med Sci 35, 651–660 (2020). https://doi.org/10.1007/s10103-019-02862-w

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