Abstract
The aim of this study is to analyze the effect of agitation of ethylenediaminetetraacetic acid (EDTA) with diode laser at different agitation times on root dentin microhardness. Eighty-four specimens were divided randomly into seven groups, as follows: (1) distilled water, (2) 17 % EDTA, (3) EDTA with 60 s ultrasonic agitation, (4) EDTA with 10 s laser agitation, (5) EDTA with 20 s laser agitation, (6) EDTA with 30 s laser agitation, and (7) EDTA with 40 s laser agitation. All of the specimens were irrigated with 5 % NaOCl and distilled water except the distilled water group. Microhardness values were calculated before and after the procedures. Statistical analyses were performed using one-way ANOVA and Tukey post hoc tests. Statistically significant differences were determined between the distilled water and other groups. Also, statistically significant differences were observed between EDTA with 40 s laser agitation and EDTA, and EDTA with 10 and 20 s laser agitations. Ultrasonic agitation of EDTA affected microhardness of root dentin similar to EDTA (p > .05). All applications decreased the microhardness of root dentin when compared with distilled water. Agitation of EDTA with diode laser for 40 s caused more reduction in microhardness of root dentin when compared with EDTA.
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Torabinejad M, Handysides R, Khademi AA, Bakland LK (2002) Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:658–666. doi:10.1067/moe.2002.128962
Vertucci FJ (1984) Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol 58:589–599
Peters OA (2004) Current challenges and concepts in the preparation of root canal systems: a review. J Endod 30:559–567
Wu MK, Wesselink PR (2001) A primary observation on the preparation and obturation of oval canals. Int Endod J 34:137–141
Wu MK, de Schwartz FB, van der Sluis LW, Wesselink PR (2001) The quality of root fillings remaining in mandibular incisors after root-end cavity preparation. Int Endod J 34:613–619
Gu LS, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR (2009) Review of contemporary irrigant agitation techniques and devices. J Endod 35:791–804. doi:10.1016/j.joen.2009.03.010
Guerisoli DM, Marchesan MA, Walmsley AD, Lumley PJ, Pecora JD (2002) Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasonic agitation. Int Endod J 35:418–421
De Moor RJ, Blanken J, Meire M, Verdaasdonk R (2009) Laser induced explosive vapor and cavitation resulting in effective irrigation of the root canal. Part 2: evaluation of the efficacy. Lasers Surg Med 41:520–523. doi:10.1002/lsm.20797
de Groot SD, Verhaagen B, Versluis M, Wu MK, Wesselink PR, van der Sluis LW (2009) Laser-activated irrigation within root canals: cleaning efficacy and flow visualization. Int Endod J 42:1077–1083. doi:10.1111/j.1365-2591.2009.01634.x
Moon YM, Kim HC, Bae KS, Baek SH, Shon WJ, Lee W (2012) Effect of laser-activated irrigation of 1320-nanometer Nd:YAG laser on sealer penetration in curved root canals. J Endod 38:531–535. doi:10.1016/j.joen.2011.12.008
Hmud R, Kahler WA, George R, Walsh LJ (2010) Cavitational effects in aqueous endodontic irrigants generated by near-infrared lasers. J Endod 36:275–278. doi:10.1016/j.joen.2009.08.012
Coluzzi DJ (2004) Fundamentals of dental lasers: science and instruments. Dent Clin North Am 48:751–770. doi:10.1016/j.cden.2004.05.003
Coluzzi DJ (2000) An overview of laser wavelengths used in dentistry. Dent Clin North Am 44:753–765
Wang X, Sun Y, Kimura Y, Kinoshita J, Ishizaki NT, Matsumoto K (2005) Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed Laser Surg 23:575–581. doi:10.1089/pho.2005.23.575
de Souza EB, Cai S, Simionato MR, Lage-Marques JL (2008) High-power diode laser in the disinfection in depth of the root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106:e68–e72. doi:10.1016/j.tripleo.2008.02.032
Saleh AA, Ettman WM (1999) Effect of endodontic irrigation solutions on microhardness of root canal dentine. J Dent 27:43–46
Ari H, Erdemir A, Belli S (2004) Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. J Endod 30:792–795
De-Deus G, Paciornik S, Mauricio MH (2006) Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine. Int Endod J 39:401–407. doi:10.1111/j.1365-2591.2006.01094.x
Lottanti S, Gautschi H, Sener B, Zehnder M (2009) Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentine and the smear layer. Int Endod J 42:335–343. doi:10.1111/j.1365-2591.2008.01514.x
Torabinejad M, Cho Y, Khademi AA, Bakland LK, Shabahang S (2003) The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer. J Endod 29:233–239. doi:10.1097/00004770-200304000-00001
Oliveira LD, Carvalho CA, Nunes W, Valera MC, Camargo CH, Jorge AO (2007) Effects of chlorhexidine and sodium hypochlorite on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:e125–e128. doi:10.1016/j.tripleo.2007.04.019
Slutzky-Goldberg I, Maree M, Liberman R, Heling I (2004) Effect of sodium hypochlorite on dentin microhardness. J Endod 30:880–882
Eldeniz AU, Erdemir A, Belli S (2005) Effect of EDTA and citric acid solutions on the microhardness and the roughness of human root canal dentin. J Endod 31:107–110
Lewinstein I, Hirschfeld Z, Stabholz A, Rotstein I (1994) Effect of hydrogen peroxide and sodium perborate on the microhardness of human enamel and dentin. J Endod 20:61–63. doi:10.1016/S0099-2399(06)81181-7
Sayin TC, Serper A, Cehreli ZC, Otlu HG (2007) The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:418–424. doi:10.1016/j.tripleo.2007.03.021
Al-Omari WM, Palamara JE (2012) The effect of Nd:YAG and Er, Cr:YSGG lasers on the microhardness of human dentin. Lasers Med Sci. doi:10.1007/s10103-012-1094-3
Spano JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pecora JD (2009) Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators. J Endod 35:727–730. doi:10.1016/j.joen.2009.02.008
Machado-Silveiro LF, Gonzalez-Lopez S, Gonzalez-Rodriguez MP (2004) Decalcification of root canal dentine by citric acid, EDTA and sodium citrate. Int Endod J 37:365–369. doi:10.1111/j.1365-2591.2004.00813.x
Kuah HG, Lui JN, Tseng PS, Chen NN (2009) The effect of EDTA with and without ultrasonics on removal of the smear layer. J Endod 35:393–396. doi:10.1016/j.joen.2008.12.007
Fusayama T, Maeda T (1969) Effect of pulpectomy on dentin hardness. J Dent Res 48:452–460
Arends J, ten Bosch JJ (1992) Demineralization and remineralization evaluation techniques. J Dent Res 71:924–928
Panighi M, G’Sell C (1993) Effect of the tooth microstructure on the shear bond strength of a dental composite. J Biomed Mater Res 27:975–981. doi:10.1002/jbm.820270802
Perinka L, Sano H, Hosoda H (1992) Dentin thickness, hardness, and Ca-concentration vs bond strength of dentin adhesives. Dent Mater 8:229–233
Tatsumi T, Inokoshi S, Yamada T, Hosoda H (1992) Remineralization of etched dentin. J Prosthet Dent 67:617–620
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Arslan, H., Yeter, K.Y., Karatas, E. et al. Effect of agitation of EDTA with 808-nm diode laser on dentin microhardness. Lasers Med Sci 30, 599–604 (2015). https://doi.org/10.1007/s10103-013-1364-8
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DOI: https://doi.org/10.1007/s10103-013-1364-8