Skip to main content

Advertisement

Log in

Effect of agitation of EDTA with 808-nm diode laser on dentin microhardness

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

The aim of this study is to analyze the effect of agitation of ethylenediaminetetraacetic acid (EDTA) with diode laser at different agitation times on root dentin microhardness. Eighty-four specimens were divided randomly into seven groups, as follows: (1) distilled water, (2) 17 % EDTA, (3) EDTA with 60 s ultrasonic agitation, (4) EDTA with 10 s laser agitation, (5) EDTA with 20 s laser agitation, (6) EDTA with 30 s laser agitation, and (7) EDTA with 40 s laser agitation. All of the specimens were irrigated with 5 % NaOCl and distilled water except the distilled water group. Microhardness values were calculated before and after the procedures. Statistical analyses were performed using one-way ANOVA and Tukey post hoc tests. Statistically significant differences were determined between the distilled water and other groups. Also, statistically significant differences were observed between EDTA with 40 s laser agitation and EDTA, and EDTA with 10 and 20 s laser agitations. Ultrasonic agitation of EDTA affected microhardness of root dentin similar to EDTA (p > .05). All applications decreased the microhardness of root dentin when compared with distilled water. Agitation of EDTA with diode laser for 40 s caused more reduction in microhardness of root dentin when compared with EDTA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Torabinejad M, Handysides R, Khademi AA, Bakland LK (2002) Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:658–666. doi:10.1067/moe.2002.128962

    Article  PubMed  Google Scholar 

  2. Vertucci FJ (1984) Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol 58:589–599

    Article  CAS  PubMed  Google Scholar 

  3. Peters OA (2004) Current challenges and concepts in the preparation of root canal systems: a review. J Endod 30:559–567

    Article  PubMed  Google Scholar 

  4. Wu MK, Wesselink PR (2001) A primary observation on the preparation and obturation of oval canals. Int Endod J 34:137–141

    Article  CAS  PubMed  Google Scholar 

  5. Wu MK, de Schwartz FB, van der Sluis LW, Wesselink PR (2001) The quality of root fillings remaining in mandibular incisors after root-end cavity preparation. Int Endod J 34:613–619

    Article  CAS  PubMed  Google Scholar 

  6. Gu LS, Kim JR, Ling J, Choi KK, Pashley DH, Tay FR (2009) Review of contemporary irrigant agitation techniques and devices. J Endod 35:791–804. doi:10.1016/j.joen.2009.03.010

    Article  PubMed  Google Scholar 

  7. Guerisoli DM, Marchesan MA, Walmsley AD, Lumley PJ, Pecora JD (2002) Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasonic agitation. Int Endod J 35:418–421

    Article  CAS  PubMed  Google Scholar 

  8. De Moor RJ, Blanken J, Meire M, Verdaasdonk R (2009) Laser induced explosive vapor and cavitation resulting in effective irrigation of the root canal. Part 2: evaluation of the efficacy. Lasers Surg Med 41:520–523. doi:10.1002/lsm.20797

    Article  PubMed  Google Scholar 

  9. de Groot SD, Verhaagen B, Versluis M, Wu MK, Wesselink PR, van der Sluis LW (2009) Laser-activated irrigation within root canals: cleaning efficacy and flow visualization. Int Endod J 42:1077–1083. doi:10.1111/j.1365-2591.2009.01634.x

    Article  PubMed  Google Scholar 

  10. Moon YM, Kim HC, Bae KS, Baek SH, Shon WJ, Lee W (2012) Effect of laser-activated irrigation of 1320-nanometer Nd:YAG laser on sealer penetration in curved root canals. J Endod 38:531–535. doi:10.1016/j.joen.2011.12.008

    Article  PubMed  Google Scholar 

  11. Hmud R, Kahler WA, George R, Walsh LJ (2010) Cavitational effects in aqueous endodontic irrigants generated by near-infrared lasers. J Endod 36:275–278. doi:10.1016/j.joen.2009.08.012

    Article  PubMed  Google Scholar 

  12. Coluzzi DJ (2004) Fundamentals of dental lasers: science and instruments. Dent Clin North Am 48:751–770. doi:10.1016/j.cden.2004.05.003

    Article  PubMed  Google Scholar 

  13. Coluzzi DJ (2000) An overview of laser wavelengths used in dentistry. Dent Clin North Am 44:753–765

    CAS  PubMed  Google Scholar 

  14. Wang X, Sun Y, Kimura Y, Kinoshita J, Ishizaki NT, Matsumoto K (2005) Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation. Photomed Laser Surg 23:575–581. doi:10.1089/pho.2005.23.575

    Article  PubMed  Google Scholar 

  15. de Souza EB, Cai S, Simionato MR, Lage-Marques JL (2008) High-power diode laser in the disinfection in depth of the root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106:e68–e72. doi:10.1016/j.tripleo.2008.02.032

    Article  PubMed  Google Scholar 

  16. Saleh AA, Ettman WM (1999) Effect of endodontic irrigation solutions on microhardness of root canal dentine. J Dent 27:43–46

    Article  CAS  PubMed  Google Scholar 

  17. Ari H, Erdemir A, Belli S (2004) Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. J Endod 30:792–795

    Article  PubMed  Google Scholar 

  18. De-Deus G, Paciornik S, Mauricio MH (2006) Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine. Int Endod J 39:401–407. doi:10.1111/j.1365-2591.2006.01094.x

    Article  CAS  PubMed  Google Scholar 

  19. Lottanti S, Gautschi H, Sener B, Zehnder M (2009) Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentine and the smear layer. Int Endod J 42:335–343. doi:10.1111/j.1365-2591.2008.01514.x

    Article  CAS  PubMed  Google Scholar 

  20. Torabinejad M, Cho Y, Khademi AA, Bakland LK, Shabahang S (2003) The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer. J Endod 29:233–239. doi:10.1097/00004770-200304000-00001

    Article  PubMed  Google Scholar 

  21. Oliveira LD, Carvalho CA, Nunes W, Valera MC, Camargo CH, Jorge AO (2007) Effects of chlorhexidine and sodium hypochlorite on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:e125–e128. doi:10.1016/j.tripleo.2007.04.019

    Article  PubMed  Google Scholar 

  22. Slutzky-Goldberg I, Maree M, Liberman R, Heling I (2004) Effect of sodium hypochlorite on dentin microhardness. J Endod 30:880–882

    Article  PubMed  Google Scholar 

  23. Eldeniz AU, Erdemir A, Belli S (2005) Effect of EDTA and citric acid solutions on the microhardness and the roughness of human root canal dentin. J Endod 31:107–110

    Article  PubMed  Google Scholar 

  24. Lewinstein I, Hirschfeld Z, Stabholz A, Rotstein I (1994) Effect of hydrogen peroxide and sodium perborate on the microhardness of human enamel and dentin. J Endod 20:61–63. doi:10.1016/S0099-2399(06)81181-7

    Article  CAS  PubMed  Google Scholar 

  25. Sayin TC, Serper A, Cehreli ZC, Otlu HG (2007) The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:418–424. doi:10.1016/j.tripleo.2007.03.021

    Article  PubMed  Google Scholar 

  26. Al-Omari WM, Palamara JE (2012) The effect of Nd:YAG and Er, Cr:YSGG lasers on the microhardness of human dentin. Lasers Med Sci. doi:10.1007/s10103-012-1094-3

    Google Scholar 

  27. Spano JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pecora JD (2009) Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators. J Endod 35:727–730. doi:10.1016/j.joen.2009.02.008

    Article  PubMed  Google Scholar 

  28. Machado-Silveiro LF, Gonzalez-Lopez S, Gonzalez-Rodriguez MP (2004) Decalcification of root canal dentine by citric acid, EDTA and sodium citrate. Int Endod J 37:365–369. doi:10.1111/j.1365-2591.2004.00813.x

    Article  CAS  PubMed  Google Scholar 

  29. Kuah HG, Lui JN, Tseng PS, Chen NN (2009) The effect of EDTA with and without ultrasonics on removal of the smear layer. J Endod 35:393–396. doi:10.1016/j.joen.2008.12.007

    Article  PubMed  Google Scholar 

  30. Fusayama T, Maeda T (1969) Effect of pulpectomy on dentin hardness. J Dent Res 48:452–460

    Article  CAS  PubMed  Google Scholar 

  31. Arends J, ten Bosch JJ (1992) Demineralization and remineralization evaluation techniques. J Dent Res 71:924–928

    PubMed  Google Scholar 

  32. Panighi M, G’Sell C (1993) Effect of the tooth microstructure on the shear bond strength of a dental composite. J Biomed Mater Res 27:975–981. doi:10.1002/jbm.820270802

    Article  CAS  PubMed  Google Scholar 

  33. Perinka L, Sano H, Hosoda H (1992) Dentin thickness, hardness, and Ca-concentration vs bond strength of dentin adhesives. Dent Mater 8:229–233

    Article  CAS  PubMed  Google Scholar 

  34. Tatsumi T, Inokoshi S, Yamada T, Hosoda H (1992) Remineralization of etched dentin. J Prosthet Dent 67:617–620

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hakan Arslan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arslan, H., Yeter, K.Y., Karatas, E. et al. Effect of agitation of EDTA with 808-nm diode laser on dentin microhardness. Lasers Med Sci 30, 599–604 (2015). https://doi.org/10.1007/s10103-013-1364-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-013-1364-8

Keywords

Navigation