Abstract
Exploring new and unfamiliar environments is critical for survival, providing information on food, shelter, mates, and sources of danger. The open field paradigm is commonly used to study exploration and anxiety-like behaviors in the lab. Many social animals, like humans and rats, may explore their environments in social groups; however, relatively few studies have investigated the influence of conspecifics on open field activity. Here, we provide a comparison of individual (solo) or pairs of male rats (dyads) exploring and interacting across repeated exposures to an unfamiliar (Day 1) or more familiar (Day 2) open field. Both solo rats and dyads explored a larger area, traveled further, and spent less time near the maze walls on the second maze exposure. Solo rats explored a larger area and spent less time near the maze walls than dyads on both days because dyads spent more time socializing rather than exploring the environment. Furthermore, we compared familiar dyads that were co-housed for seven days versus stranger dyads that met for the first time in the open field. While familiar and stranger dyads did not differ in maze exploration, strangers spent more time interacting nose to nose than nose to anogenital. These results indicate that the degree of familiarity with the environment does not interact with the tendency of dyads to socialize rather than explore the environment.
Data availability
All data supporting the findings of this study are available from the corresponding author upon reasonable request.
References
Bert B, Fink H, Sohr R, Rex A (2001) Different effects of diazepam in fischer rats and two stocks of wistar rats in tests of anxiety. Pharmacol Biochem Behav 70(2–3):411–420. https://doi.org/10.1016/S0091-3057(01)00629-3
Dorfman A, Eilam D (2018) The hierarchy of food, sociality, and experience in spatial decision-making by food-deprived rats. Behav Proc 157(July):1–6. https://doi.org/10.1016/j.beproc.2018.08.006
Dorfman A, Nielbo KL, Eilam D (2016) Traveling companions add complexity and hinder performance in the spatial behavior of rats. PLoS ONE 11(1):e0146137. https://doi.org/10.1371/journal.pone.0146137
Dorfman A, Weiss O, Hagbi Z, Levi A, Eilam D (2021) Social spatial cognition. Neurosci Biobehav Rev 121:277–290. https://doi.org/10.1016/j.neubiorev.2020.12.023
Eilam D (2014) Of mice and men: building blocks in cognitive mapping. Neurosci Biobehav Rev 47:393–409. https://doi.org/10.1016/j.neubiorev.2014.09.010
Eilam D, Golani I (1989) Home base behavior of rats (Rattus norvegicus) exploring a novel environment. Behav Brain Res 34(3):199–211. https://doi.org/10.1016/S0166-4328(89)80102-0
Galef BG, Giraldeau L-A (2001) Social influences on foraging in vertebrates: causal mechanisms and adaptive functions. Anim Behav 61(1):3–15. https://doi.org/10.1006/anbe.2000.1557
Hall C, Ballachey EL (1932) A study of the rat’s behavior in a field. A contribution to method in comparative psychology. Univ Calif Publ Psychol 6:1–12
Hughes RN (1969) Social facilitation of locomotion and exploration in rats. Br J Psychol 60(3):385–388. https://doi.org/10.1111/j.2044-8295.1969.tb01211.x
Keverne EB (2002) Pheromones, vomeronasal function, and gender-specific behavior. Cell 108(6):735–738. https://doi.org/10.1016/S0092-8674(02)00687-6
Kikusui T, Winslow JT, Mori Y (2006) Social buffering: relief from stress and anxiety. Philos Transa Royal Soc B: Biol Sci 361(1476):2215–2228. https://doi.org/10.1098/rstb.2006.1941
Kiyokawa Y (2004) Alarm pheromones with different functions are released from different regions of the body surface of male rats. Chem Senses 29(1):35–40. https://doi.org/10.1093/chemse/bjh004
Kiyokawa Y (2015) Social odors: alarm pheromones and social buffering. Soc Behav Rodents Humans. https://doi.org/10.1007/7854_2015_406
Latané B (1969) Gregariousness and fear in laboratory rats. J Exp Soc Psychol 5(1):61–69. https://doi.org/10.1016/0022-1031(69)90006-7
Mathis A, Mamidanna P, Cury KM, Abe T, Murthy VN, Mathis MW, Bethge M (2018) DeepLabCut: markerless pose estimation of user-defined body parts with deep learning. Nat Neurosci 21(9):1281–1289. https://doi.org/10.1038/s41593-018-0209-y
Miller CK, Halbing AA, Patisaul HB, Meitzen J (2021) Interactions of the estrous cycle, novelty, and light on female and male rat open field locomotor and anxiety-related behaviors. Physiol Behav 228:113203. https://doi.org/10.1016/j.physbeh.2020.113203
Nagy M, Horicsányi A, Kubinyi E, Couzin ID, Vásárhelyi G, Flack A, Vicsek T (2020) Synergistic benefits of group search in rats. Curr Biol 30(23):4733-4738.e4. https://doi.org/10.1016/j.cub.2020.08.079
Proops L, Troisi CA, Kleinhappel TK, Romero T (2021) Non-random associations in group housed rats (Rattus norvegicus). Sci Rep 11(1):15349. https://doi.org/10.1038/s41598-021-94608-4
Prut L, Belzung C (2003) The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol 463(1–3):3–33. https://doi.org/10.1016/S0014-2999(03)01272-X
Rao RP, von Heimendahl M, Bahr V, Brecht M (2019) Neuronal responses to conspecifics in the ventral CA1. Cell Rep 27(12):3460-3472.e3. https://doi.org/10.1016/j.celrep.2019.05.081
Roberts MW, Wolfe JL (1974) Social influences on susceptibility to predation in cotton rats. J Mammal 55(4):869–872. https://doi.org/10.2307/1379424
Tchernichovski O, Benjamini Y, Golani I (1996) Constraints and the emergence of “free” exploratory behavior in rat ontogeny. Behaviour 133(7–8):519–539. https://doi.org/10.1163/156853996X00198
Tchernichovski O, Benjamini Y, Golani I (1998) The dynamics of long-term exploration in the rat. Biol Cybern 78(6):423–432. https://doi.org/10.1007/s004220050446
Weiss O, Segev E, Eilam D (2015) “Shall two walk together except they be agreed?” Spatial behavior in rat dyads. Anim Cogn 18(1):39–51. https://doi.org/10.1007/s10071-014-0775-7
Weiss O, Dorfman A, Ram T, Zadicario P, Eilam D (2017) Rats do not eat alone in public: Food-deprived rats socialize rather than competing for baits. PLoS ONE 12(3):e0173302. https://doi.org/10.1371/journal.pone.0173302
Weiss O, Levi A, Segev E, Simbirsky M, Eilam D (2018) Spatio-temporal organization during group formation in rats. Anim Cogn 21(4):513–529. https://doi.org/10.1007/s10071-018-1185-z
Wesson DW (2013) Sniffing behavior communicates social hierarchy. Curr Biol 23(7):575–580. https://doi.org/10.1016/j.cub.2013.02.012
Wolfe J, Mende C, Brecht M (2011) Social facial touch in rats. Behav Neurosci 125(6):900–910. https://doi.org/10.1037/a0026165
Zajonc RB (1965) Social facilitation. Science 149(3681):269–274. https://doi.org/10.1126/science.149.3681.269
Funding
This work was supported by the University of Connecticut Research Foundation (UCRF) and the Peter and Carmen Lucia Buck Foundation (PCLB) grants to Etan J. Markus, and the Crandall-Cordero Fellowship and The Connecticut Institute for the Brain and Cognitive Sciences Graduate Fellowship to Shang Lin (Tommy) Lee.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Lee, S.T., Ahmed, S., Horbal, L. et al. Social factors influence solo and rat dyads exploration of an unfamiliar open field. Anim Cogn 26, 703–708 (2023). https://doi.org/10.1007/s10071-022-01664-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10071-022-01664-y