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Persistence of maternal milk derived Lactobacillus plantarum in the infant feces and its antagonistic activity against Escherichia coli O157:H7

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Abstract

The diversity of lactic acid bacteria (LAB) in maternal milk and feces from Thai mother-infants pairs were revealed through nested PCR-DGGE. LAB species residing in maternal milk drawn from each individual demonstrated high uniqueness, yet shared similarity to her infant. Multiple strains of L. plantarum, L. fermentum, L. rhamnosus, L. mucosae, L. casei were continuously detected, suggesting direct transfer from a mother to her infant via breastfeeding. L. plantarum, the most commonly found species with many strain variants, remained persistent in infant’s feces up to six months postpartum. Such success could be achieved through its ability to utilize fructooligosaccharides (FOS)/inulin together with antibacterial activity and competitive adhesion. With FOS/inulin, the prebiotic utilizing L. plantarum (M117 and M118) isolated from maternal milk effectively inhibited E. coli O157:H7 under highly microflora competitive and glucose-limited environments of colon model. The results introduce the potential trend for development of effective anti-diarrheal synbiotic infant formulae.

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Acknowledgements

This work was financially supported by Graduate School and Faculty of Agro-Industry, Prince of Songkla University, Hat Yai campus, Thailand and the Office of the Higher Education Commission under the CHE-PhD Scholarship program (Grant No. 03-2553). The authors would like to thank Mr. George A. Attig at the Institute of Nutrition, Mahidol University, Thailand, for his valuable assistance in English proofreading.

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  1. Biotechnology Program, Faculty of Agro-Industry, Prince of Songkla University, 15 Kanchanavanich Rd., Hat Yai, Songkhla, 90110, Thailand

    Siriporn Taweerodjanakarn, Khanitta Kongnum & Tipparat Hongpattarakere

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  1. Siriporn Taweerodjanakarn
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  2. Khanitta Kongnum
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  3. Tipparat Hongpattarakere
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Correspondence to Tipparat Hongpattarakere.

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The protocol of this study was reviewed and approved by the Ethics Committee, Faculty of Medicine, Prince of Songkla University, Thailand (EC Number: 55-243-19-2-3).

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Taweerodjanakarn, S., Kongnum, K. & Hongpattarakere, T. Persistence of maternal milk derived Lactobacillus plantarum in the infant feces and its antagonistic activity against Escherichia coli O157:H7. Food Sci Biotechnol 32, 1079–1089 (2023). https://doi.org/10.1007/s10068-023-01243-y

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