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High-yield cycloamylose production from sweet potato starch using Pseudomonas isoamylase and Thermus aquaticus 4-α-glucanotransferase

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Abstract

An optimal reaction condition for producing cycloamylose (CA) from sweet potato starch was investigated using a combination of isoamylase (from Pseudomonas sp.) and 4-α-glucanotransferase (from Thermus aquaticus, TAαGT). Starch was debranched by isoamylase for 8 h and subsequently reacted with TAαGT for 12 h. The yield and purity of CA products were determined using HPSEC and MALDI-TOFMS, respectively. Consequently, the maximum yield was 48.56%, exhibiting the highest CA production efficiency ever reported from starch. The CA products showed a wide range of the degree of polymerization (DP) with the minimum DP of 5. CA was also produced by simultaneous treatment of isoamylase and TAαGT. The yield was 3.31%, and the final products were contaminated by multiple branched and linear molecules. This result suggests that a former reaction condition (the sequential addition of isoamylase and TAαGT) is preferable for producing CA from sweet potato starch.

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Authors and Affiliations

  1. Center for Food and Bioconvergence, Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, 08826, Korea

    Sun Chu, Jung Sun Hong, Shin-Joung Rho & Yong-Ro Kim

  2. Department of Central Area Crop Science, NICS, Suwon, Gyeonggi, 16613, Korea

    Jiyoung Park

  3. Department of functional Crop, Functional Crop Resource Development Division, NICS, RDA, Miryang, Gyeongnam, 50424, Korea

    Sang-Ik Han

  4. Department of Food and Biotechnology, Korea University, Sejong, 30019, Korea

    Young-Wan Kim

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  1. Sun Chu
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Correspondence to Yong-Ro Kim.

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Chu, S., Hong, J.S., Rho, SJ. et al. High-yield cycloamylose production from sweet potato starch using Pseudomonas isoamylase and Thermus aquaticus 4-α-glucanotransferase. Food Sci Biotechnol 25, 1413–1419 (2016). https://doi.org/10.1007/s10068-016-0220-6

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  • DOI: https://doi.org/10.1007/s10068-016-0220-6

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