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Human cytomegalovirus (HCMV) US2 protein interacts with human CD1d (hCD1d) and down-regulates invariant NKT (iNKT) cell activity

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Molecules and Cells

Abstract

To avoid host immune surveillance, human cytomegalovirus (HCMV) encoded endoplasmic reticulum (ER)-membrane glycoprotein US2, which interferes with antigen presenting mechanism of Major histocompatibility complex (MHC) class Ia and class II molecules. However, not many attempts have been made to study the effect of HCMV US2 on the expression of MHC class Ib molecules. In this study, we examined the effect of HCMV US2 on the expression and function of human CD1d (hCD1d), which presents glycolipid antigens to invariant NKT (iNKT) cells. Our results clearly showed that the physiological interaction between ER lumenal domain of HCMV US2 and α3 domain of hCD1d was observed within ER. Compared with mature form of hCD1d, immature form of hCD1d is more susceptible to ubiquitin-dependent proteasomal degradation mediated by HCMV US2. Moreover, the ectopic expression of HCMV US2 leads to the down-modulation of iNKT cell activity without significant change of hCD1d expression. These results will advance our understanding of the function of HCMV US2 in immune evasive mechanisms against anti-viral immunity of iNKT cells.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Jihye Han, Jae Yeon Lee, Joonbeom Bae, Se Ho Park & Taehoon Chun

  2. Research Institute and Hospital, National Cancer Center, Goyang, 410-769, Korea

    Seung Bae Rho

  3. Department of Biomedical Laboratory Science, Gimcheon University, Gimcheon, 740-704, Korea

    Suk Jun Lee

  4. Department of Life Sciences, College of Bionano, Gachon University, Seongnam, 461-701, Korea

    Sang Yeol Lee & Jae Young Kim

  5. Department of Internal Medicine, College of Medicine, Seoul National University, Seoul, 110-799, Korea

    Curie Ahn

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  1. Jihye Han
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  2. Seung Bae Rho
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Correspondence to Taehoon Chun.

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Han, J., Rho, S.B., Lee, J.Y. et al. Human cytomegalovirus (HCMV) US2 protein interacts with human CD1d (hCD1d) and down-regulates invariant NKT (iNKT) cell activity. Mol Cells 36, 455–464 (2013). https://doi.org/10.1007/s10059-013-0221-8

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  • DOI: https://doi.org/10.1007/s10059-013-0221-8

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