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Teratocarcinoma-derived growth factor-1 (Cripto-1) is overexpressed in epithelial odontogenic lesions displaying more aggressive behaviour

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Abstract

Purpose

Cripto-1 also known as teratoma-derived growth factor 1 (TDGF-1) belongs to the EGF-CFC family of growth factor-like molecules. Cripto-1 is involved with embryonic development and not expressed in adult tissue, but some tumours are accompanied by reactivation.

Methods

The aim of this study was to evaluate the immunohistochemical expression of Cripto-1 in most common odontogenic cysts and tumours. Thirty ameloblastomas, 30 keratocysts, 30 dentigerous cysts and two ameloblastic carcinomas were evaluated using the polymeric immunoperoxidase technique. Immunohistochemical expressions were analysed by the IRS (immunoreactive score). Statistical analyses were performed by the Kruskal–Wallis and Mann–Whitney tests (p ≤ 0.05).

Results

Age ranged from 9 to 75 years old, with a prevalence of females (n = 49/53.3%). The mandible was the most affected anatomical site (n = 69/75.0%). Cripto-1 immunoexpression was observed in all ameloblastoma, keratocyst and ameloblastic carcinoma cases, although nine dentigerous cyst cases (30%) were negative. Expression scores were higher in ameloblastoma, keratocyst and ameloblastic carcinoma cases (median ranging from 8 to 11) when compared with dentigerous cyst cases (median of 2), with a statistically significant difference (p < 0.001).

Conclusions

Cripto-1 is critically important in the progression of several tumours since it is related to significant cell survival and differentiation pathways. The high expression of Cripto-1 in more aggressive odontogenic lesions suggests that this molecule may be involved in the activation of important pathways related to the etiopathogenesis of these lesions.

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Funding

This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES).

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Authors and Affiliations

  1. Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte-UFRN, Av. Sen Salgado Filho, 1787, Lagoa Nova, Natal, RN, Brazil

    Leorik Pereira da Silva, Mara Luana Batista Severo, Luiz Arthur Barbosa da Silva, Hébel Cavalcanti Galvão, Lélia Batista de Souza & Márcia Cristina da Costa Miguel

  2. Institute of Health and Biotechnology, Federal University of Amazonas-UFAM, Coari, AM, Brazil

    Leorik Pereira da Silva

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  1. Leorik Pereira da Silva
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  2. Mara Luana Batista Severo
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Correspondence to Leorik Pereira da Silva.

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This article does not contain any studies with human participants or animals performed by any of the authors. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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da Silva, L.P., Severo, M.L.B., da Silva, L.A.B. et al. Teratocarcinoma-derived growth factor-1 (Cripto-1) is overexpressed in epithelial odontogenic lesions displaying more aggressive behaviour. Oral Maxillofac Surg 24, 455–460 (2020). https://doi.org/10.1007/s10006-020-00877-0

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