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Marinobacter alkaliphilus sp. nov., a novel alkaliphilic bacterium isolated from subseafloor alkaline serpentine mud from Ocean Drilling Program Site 1200 at South Chamorro Seamount, Mariana Forearc

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Abstract

Novel alkaliphilic, mesophilic bacteria were isolated from subseafloor alkaline serpentine mud from the Ocean Drilling Program (ODP) Hole 1200D at a serpentine mud volcano, South Chamorro Seamount in the Mariana Forearc. The cells of type strain ODP1200D-1.5T were motile rods with a single polar flagellum. Growth was observed between 10 and 45–50°C (optimum temperature: 30–35°C, 45-min doubling time), between pH 6.5 and 10.8–11.4 (optimum: pH 8.5–9.0), and between NaCl concentrations of 0 and 21% (w/v) (optimum NaCl concentration: 2.5–3.5%). The isolate was a facultatively anaerobic heterotroph utilizing various complex substrates, hydrocarbons, carbohydrates, organic acids, and amino acids. Nitrate or fumarate could serve as an electron acceptor to support growth under anaerobic conditions. The G+C content of the genomic DNA was 57.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Marinobacter and was the most closely related to M. aquaeolei strain VT8T and M. hydrocarbonoclasticus strain SP.17T, while DNA–DNA hybridization demonstrated that the new isolate could be genetically differentiated from the previously described species of Marinobacter. Based on the physiological and molecular properties of the new isolate, we propose the name Marinobacter alkaliphilus sp. nov., type strain: ODP1200D-1.5T (JCM12291T and ATCC BAA-889T).

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Acknowledgements

We would like to thank Mr. Katsuyuki Uematsu for assistance in preparing electron micrographs. We are very grateful to the clues of DSDV Joides Resolution and the staff scientists of the ODP Leg 195 for helping us to collect subseafloor core samples. This research was funded in part by the US Science Support Program of ODP and by a summer research grant from Western Washington University (to Moyer).

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Authors and Affiliations

  1. Subground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan

    Ken Takai, Masayuki Miyazaki, Yuichi Nogi, Hisako Hirayama, Kenneth H. Nealson & Koki Horikoshi

  2. Biology Department, Western Washington University, Biology Building MS# 9160, Bellingham, WA, 98225, USA

    Craig L. Moyer

  3. Department of Earth Sciences, University of Southern California, 3651 Trousdale Pkwy., Los Angeles, CA, 90089-0740, USA

    Kenneth H. Nealson

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  1. Ken Takai
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  2. Craig L. Moyer
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  3. Masayuki Miyazaki
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  4. Yuichi Nogi
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  5. Hisako Hirayama
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  6. Kenneth H. Nealson
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  7. Koki Horikoshi
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Correspondence to Ken Takai.

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Communicated by W.D. Grant

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Takai, K., Moyer, C.L., Miyazaki, M. et al. Marinobacter alkaliphilus sp. nov., a novel alkaliphilic bacterium isolated from subseafloor alkaline serpentine mud from Ocean Drilling Program Site 1200 at South Chamorro Seamount, Mariana Forearc. Extremophiles 9, 17–27 (2005). https://doi.org/10.1007/s00792-004-0416-1

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