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Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis

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Abstract

Objectives

The inhibition of bone destruction is one of the main goals of periodontitis treatment. The aim of this study was to investigate the protective effects of non-thermal atmospheric plasma (NTAP) on alveolar bone loss radiographically, histomorphometrically, and histologically in experimental periodontitis in rats.

Materials and methods

A total of twenty-eight rats were randomly divided into three groups: control group (CG) (n = 8), periodontitis group (PG) (n = 10), and NTAP group (NTAPG) (n = 10). In PG and NTAPG, experimental periodontitis was created with ligating. The kINPen 11 plasma jet was applied around the ligatured teeth in NTAPG. The samples from each group were radiographically assessed with microcomputed tomography (micro-CT); then, histological (presence of osteoclasts and inflammatory cells) and immunohistochemical (immunoreactive of OCN and ALP) findings were compared.

Results

The results revealed a significant increase in alveolar bone loss in the PG compared with CG and NTAPG (p < 0.05). Inflammation, alveolar resorption, and cement damage were reduced significantly in the group treated with NTAP compared to the PG (p < 0.05). Significantly higher levels of osteoclasts were detected in the PG in comparison with both CG and NTAPG (p < 0.05). The lowest osteocalcin and ALP values were determined in PG, and the differences between PG and both groups were also significant (p < 0.05).

Conclusion

Within the limitations of the present study, we can say that NTAP may enhance the bone remodeling process by inhibiting inflammation and preventing alveolar bone destruction.

Clinical relevance

NTAP has clinical potential for accelerating and treating periodontitis with the inflammatory response modulation, osteoblast differentiation, and alveolar bone loss reduction.

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Funding

This study was supported by the Scientific Research Project Coordination Center of Eskisehir Osmangazi University in Eskisehir, Turkey (grant number 2019-2479).

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Authors and Affiliations

  1. Faculty of Dentistry, Department of Periodontology, Eskisehir Osmangazi University, Eskisehir, Turkey

    Basak Kusakcı-Seker & Hakan Ozdemir

  2. Faculty of Medicine, Department of Histology and Embryology, Kütahya Health Science University, Kütahya, Turkey

    Suna Karadeniz-Saygili

Authors
  1. Basak Kusakcı-Seker
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  2. Hakan Ozdemir
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  3. Suna Karadeniz-Saygili
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Corresponding author

Correspondence to Basak Kusakcı-Seker.

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The experimental protocol and design were performed in accordance with guidelines laid down by the National Institutes of Health in the USA regarding the care and use of animals for experimental procedures or with the European Communities Council Directive (2010/63/EU) and approved by the Animal Research Ethics Committee of Eskisehir Osmangazi University (Protocol No. 661–1/2019). The study protocol and manuscript were created according to the “NC3Rs ARRIVE Guidelines, Animal Research: Reporting of in Vivo Experiments.”

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Kusakcı-Seker, B., Ozdemir, H. & Karadeniz-Saygili, S. Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis. Clin Oral Invest 25, 6949–6959 (2021). https://doi.org/10.1007/s00784-021-04203-0

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  • DOI: https://doi.org/10.1007/s00784-021-04203-0

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