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In situ evaluation of desensitizing toothpastes for protecting against erosive tooth wear and its characterization

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Abstract

Objective

This five-phase, single-blind, crossover in situ trial evaluated the effects of desensitizing or anti-erosive toothpastes on dentin erosive wear and tubule occlusion.

Materials and methods

Some characteristics such as relative dentin abrasivity (RDA), viscosity (V), cytotoxicity, and fluoride and calcium (Ca) availability of the toothpastes were also tested. Samples were positioned on removable intraoral appliances from 15 volunteers (n=4), according to the groups, C-control (0 ppm fluoride), F-sodium monofluorophosphate (MFP), A-MFP and arginine+calcium carbonate, CSP-calcium sodium phosphosilicate, and CS-MFP and calcium silicate+sodium phosphate. Erosion-abrasion cycling was performed (1% citric acid, 2min, 6×/day; toothbrushing, 5s, 2×/day). Surface loss (SL) was evaluated by optical profilometry. Environmental scanning electron microscopy images (ESEM) counted the number of open dentin tubules (ODTs). Data were statistically analyzed (α=0.05).

Results

There were no significant differences in SL among groups (p=0.468). The ODT for CSP, CS, and A groups were significantly lower than the control. CSP and CS presented significantly less ODT than F, but they did not significantly differ from A. F and CS were more abrasive than all the other toothpastes; CSP was more abrasive than A, and C was the least abrasive. CSP presented higher V (p<0.05). All toothpastes presented lower cell viability than the control without toothpaste exposure. F and A presented significantly higher values of F- (p<0.05). All fluoride toothpastes had similar amount of Ca, differing from the control (p<0.05).

Conclusion

Although the toothpastes caused similar degree of erosive wear, CSP and CS were able to occlude the dentin tubules.

Clinical relevance

Desensitizing toothpastes containing calcium, sodium, phosphate, and silicate could be a suitable option for treating dentin hypersensitivity.

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Change history

  • 31 May 2021

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Acknowledgements

We would like to thank the State of São Paulo Research Foundation (FAPESP) for the scholarship awarded to the first author (Grant #2015/15629-7) and Prof. Anderson Hara for the RDA test performed at the Oral Health Research Institute, Indiana University School of dentistry. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Funding

The work was supported by the State of São Paulo Research Foundation (FAPESP) Grant #2015/15629-7.

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Authors and Affiliations

  1. Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227 Cidade Universitária, São Paulo, SP, 05508-000, Brazil

    Raquel Marianna Lopes, T. Scaramucci & Ana Cecilia Correa Aranha

  2. Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, 1121 Michigan Street, Indianapolis, IN, 46202-5186, USA

    Clandler L. Walker & S. A. Feitosa

  3. School of Dentistry, Special Laboratory of Lasers in Dentistry (LELO), University of São Paulo, Avenida Professor Lineu Prestes, 2226 – Cidade Universitária, Butantã, São Paulo, Brazil

    Ana Cecilia Correa Aranha

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  1. Raquel Marianna Lopes
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  2. T. Scaramucci
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  4. S. A. Feitosa
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  5. Ana Cecilia Correa Aranha
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Contributions

Conceived and designed the experiments: RL, TS, SF, and AC

Performed the experiments: RL

Analyzed the data: TS, RL

Wrote the paper: RL, TS, SF, and AC

Corresponding author

Correspondence to Ana Cecilia Correa Aranha.

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Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

Conflict of interest

Author RML declares that she has no conflict of interest. Author TS declares that he has no conflict of interest. Author CW declares that he has no conflict of interest. Author SF declares that he has no conflict of interest.

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Lopes, R.M., Scaramucci, T., Walker, C.L. et al. In situ evaluation of desensitizing toothpastes for protecting against erosive tooth wear and its characterization. Clin Oral Invest 25, 6857–6870 (2021). https://doi.org/10.1007/s00784-021-03975-9

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