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Periradicular inflammatory response, bone resorption, and cementum repair after sealing of furcation perforation with mineral trioxide aggregate (MTA Angelus™) or Biodentine™

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Abstract

Objective

This study assessed tissue responses after furcation perforation and immediate sealing with either Biodentine™ or MTA Angelus™.

Material and methods

Sixty male Wistar rats were used (n = 6 per group/period). The mandibular first molars had the furcation mechanically exposed and sealed with either MTA or Biodentine™ and restored with silver amalgam. In an additional test group, teeth were sealed only with Biodentine™. Furcation sealing with gutta-percha and silver amalgam restoration served as positive control, and healthy untreated teeth were the negative control. Histological evaluation was performed after 14 or 21 days. Kruskal-Wallis and Dunn’s post hoc tests were performed to analyze the extent and intensity of tissue inflammation, bone resorption, and cementum repair (p < 0.05).

Results

Biodentine™ and MTA presented satisfactory results, showing a milder inflammatory response when compared to the control, regardless of the material used for coronal sealing and of the experimental period evaluated (p < 0.0001). All test groups showed less bone resorption than the positive control after 21 days (p < 0.05), and such differences were more pronounced in teeth restored with silver amalgam. Cementum repair was performed in 30% of MTA and Biodentine™ samples but not carried out in any positive control specimen.

Conclusions

Biodentine™ and MTA promoted similar responses when used to seal furcation perforations and should therefore be regarded as a promising alternative.

Clinical relevance

Knowledge about tissue responses to restorative materials is essential for improving root perforation sealing protocols. The present results showed that both Biodentine™ and MTA promoted appropriate periradicular tissue reactions in a preclinical test for evaluating furcation perforation treatments.

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Authors and Affiliations

  1. School of Dentistry, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil

    Magda de Sousa Reis

  2. School of Dentistry, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos, 2492., Porto Alegre, RS, Brazil

    Roberta Kochenborger Scarparo

  3. Department of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Liviu Steier

  4. Department of Oral Biology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    José Antônio Poli de Figueiredo

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  1. Magda de Sousa Reis
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  2. Roberta Kochenborger Scarparo
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  3. Liviu Steier
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  4. José Antônio Poli de Figueiredo
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Correspondence to Roberta Kochenborger Scarparo.

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This study was approved by the Animal Care and Use Committee of the Pontifical Catholic University of Rio Grande do Sul, Brazil (Process: 12/00320).

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de Sousa Reis, M., Scarparo, R.K., Steier, L. et al. Periradicular inflammatory response, bone resorption, and cementum repair after sealing of furcation perforation with mineral trioxide aggregate (MTA Angelus™) or Biodentine™. Clin Oral Invest 23, 4019–4027 (2019). https://doi.org/10.1007/s00784-019-02833-z

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