Skip to main content
Log in

NMR Study of Water Distribution inside Tomato Cells: Effects of Water Stress

  • Published:
Applied Magnetic Resonance Aims and scope Submit manuscript

Abstract

Tomato pericarp tissue was studied by low-field nuclear magnetic resonance (NMR) relaxometry. Two kinds of experiments were performed to investigate the correlation between multi-exponential NMR relaxation and the subcellular compartments. The longitudinal (T 1) versus transverse (T 2) relaxation times were first measured on fresh samples and then the transverse relaxation time was measured on samples exposed to water stress. Four signal components were found in all experiments. The results showed that all signal components corresponded to the water in different cell compartments, and that no signal from non-exchangeable protons was present. Moreover, we demonstrated that NMR relaxation is suitable for the continuous monitoring of water rebalancing between subcellular compartments of plant tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. B.P. Hills, S.L. Duce, Magn. Reson. Imaging 8(3), 321–331 (1990)

    Google Scholar 

  2. J.E.M. Snaar, H. Van As, Biophys. J. 63, 1654–1658 (1992)

    Google Scholar 

  3. S.Y.S. Tu, Y.J. Choi, M.J. McCarthy, K.L. McCarthy, Postharvest Biol. Technol. 44(2), 157–164 (2007)

    Google Scholar 

  4. F. Duval, M. Cambert, F. Mariette, Appl. Magn. Reson. 28(1–2), 29–40 (2005)

    Google Scholar 

  5. N.A. Marigheto, G.K. Moates, M.E. Furfaro, K.W. Waldron, B.P. Hills, Appl. Magn. Reson. 36(1), 35–47 (2009)

    Google Scholar 

  6. M. Musse, S. Quellec, M.-F. Devaux, M. Cambert, M. Lahaye, F. Mariette, Magn. Reson. Imaging 27(5), 709–719 (2009)

    Google Scholar 

  7. M. Musse, S. Quellec, M. Cambert, M.-F. Devaux, M. Lahaye, F. Mariette, Postharvest Biol. Technol. 53(1–2), 22–35 (2009)

    Google Scholar 

  8. B.P. Hills, B. Remigereau, Int. J. Food Sci. Technol. 32(1), 51–61 (1997)

    Google Scholar 

  9. N. Marigheto, L. Venturi, B. Hills, Postharvest Biol. Technol. 48(3), 331–340 (2008)

    Google Scholar 

  10. T.A. Sibgatullin, A.V. Anisimov, P.A. de Jager, F.J. Vergeldt, E. Gerkema, H. Van As, Biofizika 52(2), 268–276 (2007)

  11. F. Mariette, C. Brannelec, O. Vitrac, P. Bohuon, in Les Produits Alimentaires et l’eau, Agoral 99 (Edition Tec and Doc, Nantes, 1999)

  12. M.E. Furfaro, N. Marigheto, G.K. Moates, K. Cross, M.L. Parker, K.W. Waldron, B.P. Hills, Appl. Magn. Reson. 35(4), 521–535 (2009)

    Google Scholar 

  13. A. Raffo, R. Gianferri, R. Barbieri, E. Brosio, Food Chem. 89(1), 149–158 (2005)

  14. M. Iwaya-Inoue, R. Matsui, N. Sultana, K. Saitou, K. Sakaguchi, M. Fukuyama, J. Agron. Crop Sci. 190(1), 65–72 (2004)

    Google Scholar 

  15. D. Capitani, F. Brilli, L. Mannina, N. Proietti, F. Loreto, Plant Physiol. 149(4), 1638–1647 (2009)

    Google Scholar 

  16. S. Godefroy, P.T. Callaghan, Magn. Reson. Imaging 21(3–4), 381–383 (2003)

    Google Scholar 

  17. F. Mariette, J.P. Guillement, C. Tellier, P. Marchal, in Signal Treatment and Signal Analysis in NMR, ed. by D.N. Rutledge (Elsevier, Paris, 1996), pp. 218–234

  18. W.G. Hopkins, Physiologie Végétale (Wiley, De Boeck Université, 2003)

  19. M.D. Hurlimann, L. Burcaw, Y.Q. Song, J. Colloid Interface Sci. 297(1), 303–311 (2006)

    Google Scholar 

  20. L. Van der Weerd, M. Claessens, H. Van As, Plant Cell Environ. 25, 1539–1549 (2002)

    Google Scholar 

Download references

Acknowledgments

We wish to thank CTIFL, especially Mr. Letard, for their collaboration. We also thank Miss Drouet for her contribution to the experimental work.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Maja Musse.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Musse, M., Cambert, M. & Mariette, F. NMR Study of Water Distribution inside Tomato Cells: Effects of Water Stress. Appl Magn Reson 38, 455–469 (2010). https://doi.org/10.1007/s00723-010-0139-7

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00723-010-0139-7

Keywords

Navigation