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In-depth proteome analysis reveals multiple pathways involved in tomato SlMPK1-mediated high-temperature responses

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Abstract

High temperature (HT) is one of the major environmental factors which limits plant growth and yield. The mitogen-activated protein kinase (MAPK) plays vital roles in environmental stress responses. However, the mechanisms triggered by MAPKs in plants in response to HT are still extremely limited. In this study, the proteomic data of differences between SlMPK1 RNA-interference mutant (SlMPK1i) and wild type and of tomato (Solanum lycopersicum) plants under HT stress using isobaric tags for relative and absolute quantitation (iTRAQ) was re-analyzed in depth. In total, 168 differently expressed proteins (DEPs) were identified in response to HT stress, including 38 DEPs only found in wild type, and 84 DEPs specifically observed in SlMPK1i after HT treatment. The majority of higher expression of 84 DEPs were annotated into photosynthesis, oxidation-reduction process, protein folding, translation, proteolysis, stress response, and amino acid biosynthetic process. More importantly, SlMPK1-mediated photosynthesis was confirmed by the physiological characterization of SlMPK1i with a higher level of photosynthetic capacity under HT stress. Overall, the results reveal a set of potential candidate proteins helping to further understand the intricate regulatory network regulated by SlMPK1 in response to HT.

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Funding

This work was supported by the China Postdoctoral Science Foundation funded project (20110491464, 2012T50520), National Natural Science Foundation of China (grant no. 31101092), and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

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Authors and Affiliations

  1. Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China

    Haidong Ding, Yuan Wu, Guibo Yuan, Shuangrong Mo, Qi Chen, Xiaoying Xu, Xiaoxia Wu & Cailin Ge

  2. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China

    Haidong Ding, Xiaoying Xu, Xiaoxia Wu & Cailin Ge

Authors
  1. Haidong Ding
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  2. Yuan Wu
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  3. Guibo Yuan
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  4. Shuangrong Mo
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  5. Qi Chen
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  6. Xiaoying Xu
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  7. Xiaoxia Wu
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  8. Cailin Ge
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Contributions

H.D. and C.G. conceived of the study. H.D. and Y.W. performed most of the experiment contents. G.Y. and X.W. analyzed part of the data. Other authors assisted in experiments. H.D. wrote the manuscript. C.G. guided the manuscript writing.

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Correspondence to Haidong Ding.

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Fig. S1

Putative amino acid metabolic processing pathway regulated by SlMPK1 suppression under high temperature (HT) stress. A putative amino acid metabolic processing pathway was constructed based on KEGG mapping. The red arrows represent up-regulated differentially expressed proteins (DEPs); the blue arrows indicate proteins identified as down-regulated DEPs. (PNG 1151 kb)

High resolution image (TIF 124 kb)

Fig. S2

Putative endoplasmic reticulum protein processing pathway regulated by SlMPK1 suppression under high temperature (HT) stress. A putative endoplasmic reticulum protein processing pathway was constructed based on KEGG mapping. The red arrows represent up-regulated differentially expressed proteins (DEPs). (PNG 1676 kb)

High resolution image (TIF 121 kb)

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Ding, H., Wu, Y., Yuan, G. et al. In-depth proteome analysis reveals multiple pathways involved in tomato SlMPK1-mediated high-temperature responses. Protoplasma 257, 43–59 (2020). https://doi.org/10.1007/s00709-019-01419-6

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