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Aminolevulinic acid and nitric oxide regulate oxidative defense and secondary metabolisms in canola (Brassica napus L.) under drought stress

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Abstract

To minimize the damaging effects of stresses, plant growth regulators (PGRs) are widely used to sustain the plant life under stress-prone environments. So, a study was carried out to evaluate the response of two canola (Brassica napus L.) cultivars, Dunkeld and Cyclone, to foliar-applied two potential PGRs, nitric oxide (NO) and 5-aminolevulinic acid, under water deficit conditions. In this study, the levels of NO and ALA used were 0.02 and 0.895 mM, respectively. Plants of both canola cultivars were subjected to control (100% field capacity) and water deficit (60% field capacity). Drought stress significantly decreased growth, chlorophyll pigments, relative water contents (RWC), and soluble proteins, while it increased relative membrane permeability (RMP), proline, glycinebetaine (GB), malondialdehyde (MDA), total phenolics, and activities of catalase (CAT) and peroxidase (POD) enzymes in both cultivars. Foliar application of PGRs improved growth, chlorophyll a, GB, total phenolics, CAT activity, and total soluble proteins, while it decreased RMP, MDA, and POD activity in both canola cultivars. Other physio-biochemical attributes such as chlorophyll b, RWC, hydrogen peroxide (H2O2) and proline contents as well as superoxide dismutase (SOD) activity remained unaffected due to application of PGRs. So, the results of the present study suggest that exogenous application of NO and ALA could be useful to enhance the drought tolerance of canola plants by up-regulating the oxidative defense system, osmoprotectant accumulation, and minimizing the lipid peroxidation.

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Authors and Affiliations

  1. Department of Botany, Government College University, Faisalabad, 38000, Pakistan

    Nudrat Aisha Akram, Majid Iqbal, Atta Muhammad & Sidra Shafiq

  2. Pakistan Science Foundation, Islamabad, Pakistan

    Muhammad Ashraf

  3. Department of Botany& Microbiology, King Saud University, Riyadh, Saudi Arabia

    Fahad Al-Qurainy

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  1. Nudrat Aisha Akram
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  2. Majid Iqbal
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  3. Atta Muhammad
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  4. Muhammad Ashraf
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  5. Fahad Al-Qurainy
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Correspondence to Nudrat Aisha Akram.

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Akram, N.A., Iqbal, M., Muhammad, A. et al. Aminolevulinic acid and nitric oxide regulate oxidative defense and secondary metabolisms in canola (Brassica napus L.) under drought stress. Protoplasma 255, 163–174 (2018). https://doi.org/10.1007/s00709-017-1140-x

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