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Going "open" with Mesoscopy: a new dimension on multi-view imaging

  • Special Issue: New/Emerging Techniques in Biological Microscopy
  • Published:
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Abstract

OpenSPIM and OpenSpinMicroscopy emerged as open access platforms for Light Sheet and Optical Projection Imaging, often called as optical mesoscopy techniques. Both projects can be easily reproduced using comprehensive online instructions that should foster the implementation and further development of optical imaging techniques with sample rotation control. This additional dimension in an open system offers the possibility to make multi-view microscopy easily modified and will complement the emerging commercial solutions. Furthermore, it is deeply based on other open platforms such as MicroManager and Arduino, enabling development of tailored setups for very specific biological questions. In our perspective, the open access principle of OpenSPIM and OpenSpinMicroscopy is a game-changer, helping the concepts of light sheet and optical projection tomography (OPT) to enter the mainstream of biological imaging.

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Abbreviations

3D:

Three dimensional

4D:

Four dimensional

ASI:

Applied Scientific Instrumentation

CAD:

Computer aided design

CCD:

Charged coupled device

CO2 :

Carbon dioxide

CPU:

Central processing unit

CT:

Computed tomography

DSLM:

Digital scanned light microscopy

EMBL:

European Molecular Biology Laboratory

Gb:

Gigabyte

GPU:

Graphics processing unit

I/O:

Input/output

iSPIM:

Inverted selective plane illumination microscopy

KIT:

Karlsruhe Institute of Technology

LED:

Light-emitting diode

mSPIM:

Multidirectional selective plane illumination microscopy

NA:

Numerical aperture

OPFOS:

Orthogonal-plane fluorescence optical sectioning

OPT:

Optical projection tomography

PMT:

Photon multiplier tube

RAMM:

Rapid automated modular microscope

sCMOS:

Scientific complementary metal–oxide semiconductor

SIM:

Structured illumination microscopy

SPIM:

Selective plane illumination microscopy

Tb:

Terabyte

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Acknowledgements

E.J.G. acknowledges support from the Fundação para a Ciência e a Tecnologia grant SFRH/BPD/80717/2011. GGM acknowledges the support of the EMBO practical course on 3D Developmental Imaging, and of the Microscopy Unit of Faculdade de Ciências, University of Lisbon. The Ditassa burchelli flower was kindly provided by Prof. Lia Ascensão.

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Authors and Affiliations

  1. Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, 2780-156, Oeiras, Portugal

    Emilio Gualda, Nuno Moreno & Gabriel G. Martins

  2. Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany

    Pavel Tomancak

  3. Centro de Biologia Ambiental, Faculdade de Ciencias da Universidade de Lisboa, 1749-016, Campo Grande, Portugal

    Gabriel G. Martins

Authors
  1. Emilio Gualda
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  2. Nuno Moreno
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  3. Pavel Tomancak
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  4. Gabriel G. Martins
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Correspondence to Nuno Moreno.

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Handling Editor: J. W. Borst

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Gualda, E., Moreno, N., Tomancak, P. et al. Going "open" with Mesoscopy: a new dimension on multi-view imaging. Protoplasma 251, 363–372 (2014). https://doi.org/10.1007/s00709-013-0599-3

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  • DOI: https://doi.org/10.1007/s00709-013-0599-3

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